Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,508	+C	intergenic (+85/‑79)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,506	1	.	C	100.0%	133.8 / NA	45	intergenic (+83/‑81)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/23);  total (22/23)

AAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGA  >  NC_002947/1499365‑1499642                                                                                                                                                  |                                                                                                                                         aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCAcg‑cccg                                                                                                                                      <  1:403737/147‑3 (MQ=25) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGAGGGCAcg‑cccgct                                                                                                                                    >  4:283856/1‑145 (MQ=25)      cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCca                                                                                                                                 >  5:100936/1‑148 (MQ=25)           tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCCTGCCCGCGCAACAGGCGCCCCGGTGCATGGCCCCGGCGTTTCCCGTGT‑TAGCGGGGCCGCCCCCTCCCACAGa                                                                                                                             >  6:384915/1‑147 (MQ=255)              ggCCTTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCCCGAAACAGGCCCCGCGGTGCATGGCACCGGCTTTTCCGGTGT‑TCCCGGGCACGCCCGCTCCCACAGACAGAt                                                                                                                        >  6:347042/1‑149 (MQ=21)                  aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACACGCGCCCCGGTGCATGGCACCGGCGTTTCCGGGGT‑TAGCGGGCCCGCCCGCACCCCCACACAGATTGc                                                                                                                     >  2:84465/1‑148 (MQ=21)                         gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGc                                                                                                             <  1:84465/149‑1 (MQ=21)                            tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTACATGGCACCGGCTTTGCCGGTGT‑TCGCCGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCggg                                                                                                          >  8:419339/1‑149 (MQ=21)                             gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGT‑TAGCGGGCACGCCCGCGGCCACAGACTGATTGGCATTAAGCGGGc                                                                                                         >  3:367155/1‑149 (MQ=21)                              aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc                                                                                                         <  5:384915/148‑1 (MQ=21)                              aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc                                                                                                         <  8:77843/148‑1 (MQ=21)                                 gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt                                                                                                     >  5:218489/1‑149 (MQ=21)                                              cacaGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg                                                                                        >  5:330893/1‑149 (MQ=21)                                                  gcagGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                                                    <  3:108485/149‑1 (MQ=21)                                                      gACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                                                           >  6:353844/1‑122 (MQ=18)                                                      gACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                                                           <  5:353844/122‑1 (MQ=18)                                                      gACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCCGTGCATTGCACCCGCTTTTCCGGTGT‑TAGCGGGCACGCCCGCACCCACAGACAGAATGCCATTAAGCCGGCCGATCGCTAAAGTGACGGCCCGgtt                                                                                >  8:87067/1‑149 (MQ=18)                                                       aCTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACACGCGCCCCGGTGCATGGCACCCGCTTTTCCGGTGT‑TAGCGGGCACGCCCCCTCCCACAAACATATTGCCATTAAGCGGGCCGAAAGCTACAGTGACCGCCCGtttt                                                                               >  4:377554/1‑149 (MQ=18)                                                        cTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt                                                                              <  7:374951/149‑1 (MQ=21)                                                            cgccgCCAAACGGGTGGTCGGGGGGGGGCCCGCTAAACTGGCGCCGGGGTGCATGGCCCGGGCTTTGCGGTTGTGG‑GGGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                                                           <  6:18399/145‑1 (MQ=255)                                                              cTGCCAAAC‑GGTGGTAGGGGGCGTGCCGGCGAAACTGGGCCCGCGGTCCATGGCACGGGCTTTGCCGGTGT‑TCGGGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc                                                                        <  5:347042/149‑1 (MQ=255)                                                                              aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCCCCGGCTTTGCCGGTGT‑TAGCGGGCCCGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCGATCGCGACCGGGGCGGCCCGGTTTTTGTTGACTCGCGAACGAATCa                                                         >  2:364303/1‑149 (MQ=18)                                                                              aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGGTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                                                         >  5:382313/1‑149 (MQ=25)                                                                               gCGGGCGTGCCCGCGAAACAGGCGGCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGTCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                        <  1:333074/149‑1 (MQ=21)                                                                               gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                        <  8:131643/149‑1 (MQ=25)                                                                               gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                        <  6:218489/149‑1 (MQ=25)                                                                                cGGGCGTGCCCGCGAAACAGGCGCCGCGTTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATg                                                       <  7:87067/149‑1 (MQ=25)                                                                                 gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                      <  7:276473/149‑1 (MQ=37)                                                                                         ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                                >  5:244348/1‑147 (MQ=37)                                                                                         ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                              <  6:100936/149‑1 (MQ=37)                                                                                          cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt                                               >  1:5583/1‑147 (MQ=37)                                                                                          cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt                                               <  2:5583/147‑1 (MQ=37)                                                                                                      gccgcGGTACATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg                                 <  7:419339/149‑1 (MQ=37)                                                                                                       ccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc                                <  6:79091/149‑1 (MQ=37)                                                                                                        cgcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg                                 >  2:64660/1‑147 (MQ=37)                                                                                                           ggTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt                            <  8:194841/149‑1 (MQ=38)                                                                                                               cATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa                                           >  6:248144/1‑130 (MQ=37)                                                                                                               cATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa                                           <  5:248144/130‑1 (MQ=37)                                                                                                                    caccGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGt                   >  3:159797/4‑149 (MQ=38)                                                                                                                     accGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCGTCGCTAACGAATCATGACTCCCTTAGCACGCTATCAAGCAGATCTGAAACGTc                  >  5:357818/3‑149 (MQ=38)                                                                                                                     accGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc                  >  3:423380/3‑149 (MQ=38)                                                                                                                      ccGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc                  >  4:261038/2‑148 (MQ=39)                                                                                                                      ccGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc                  <  3:261038/147‑1 (MQ=39)                                                                                                                       cGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc                <  6:382313/149‑1 (MQ=39)                                                                                                                               ccGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtct         <  3:377554/148‑1 (MQ=255)                                                                                                                                   gtgttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa     <  4:385230/142‑1 (MQ=255)                                                                                                                                      ttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGa  >  6:167558/5‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                         AAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGA  >  NC_002947/1499365‑1499642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑