| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,126,647 | +C | intergenic (‑101/+48) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 95.2% | 135.0 / 0.1 | 42 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (15/25); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAA‑C‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126498‑1126787 | tGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCCTTCGCGG‑GTAAA‑c > 7:394097/1‑149 (MQ=255) gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGCAAGCGGCGGTATAGCCCCCATCGCCTTAGCGG‑GTTAA‑c‑cc > 6:168001/1‑148 (MQ=255) gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAA‑c‑cc > 4:359630/1‑148 (MQ=255) ggggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTATACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAA‑c‑ccgc < 5:61710/149‑4 (MQ=255) ggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAA‑c‑ccgctc < 3:359630/149‑6 (MQ=255) gatgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTac < 8:394097/149‑1 (MQ=255) tgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCCCATTCCGAGGTTTAGGCACCAACGCAATCGCGGCGTAAC‑C‑CGCGCCttccc > 4:139204/1‑145 (MQ=255) aCGCTCAGTGACATGTGGACCGCCTGCCAGGTTGTTGGGGCGACATTGAGCCCTGTAGGCGCGGGTTAACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGt < 1:392799/149‑1 (MQ=38) aCGCGCCGTCACAGGTGCCCCGCCTCCCCGGTTGGTGGGGGCACATTGCCCCCTGTCGGCGCGGGTTTACCCGCGAATGCGGCGGTATAGCCACCATCGCATTCGCGG‑TAAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGt < 8:368027/149‑1 (MQ=16) tCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGCATGCGGAGGTATAGCCACCATAGCATTAGCGG‑GGAAACC‑CGCTCCGACACAGAGCCCGCCCCCCCAGCCAGGGGGCg > 6:427320/1‑149 (MQ=255) aGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCg < 5:168001/147‑1 (MQ=255) aCATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAgg < 8:132727/149‑1 (MQ=255) aCATGTGCACCTCCTGCCAGGTTGTT‑GGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAgg < 7:421923/148‑1 (MQ=255) gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTaca > 8:114652/1‑111 (MQ=255) gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTaca < 7:114652/111‑1 (MQ=255) tgGACGTCGTGCCAGGTTGTGGGGGGGAAATTGAGCCCTGTCGGCGCGGGTTTCCCGGCTAATGCGGAGGTATAGCCACCATCGCATTGGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTAt < 2:296259/149‑1 (MQ=37) gACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCGAAATCGGACGTATATACACCAAACCAATACCCG‑GTAAA‑CACCCAACAACACACAGCCCCCCACCCCAACCACGTAGCGGGGAGGGCTAta > 8:299375/1‑147 (MQ=255) ggTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGGGGGGGGATAGCCACCAACCCATTAGCGG‑GTAAACC‑CGCGCCGACCAAGAGCCCCCCCCCCAACCCAGGGGGCGGGGGGGGCTATGAGGCGGCCTGa > 4:341787/1‑147 (MQ=255) ggTGCACATTGAGCCCTGTCGGCGCGGGTTACCCCGCGAATGCGGAGGTATACCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGGCCTGATAGCTCtcga < 3:257247/149‑1 (MQ=38) cATTGAGCCCTGTAGGCGCGGGTTTACCTGCTAATGCGGAGGTATAGCCACCATCGCATTCGGGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGAc < 2:143015/148‑1 (MQ=39) aTTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACgg < 2:11378/149‑1 (MQ=255) ttGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACggg < 3:405379/149‑1 (MQ=255) tAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGcac < 8:28398/148‑1 (MQ=255) gcgcGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACgg < 5:96082/135‑1 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 7:321806/1‑149 (MQ=255) gAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGaa > 8:46444/1‑149 (MQ=255) gAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGaa < 5:313429/149‑1 (MQ=255) gCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACg > 3:43640/1‑149 (MQ=255) cGGAGGTTTAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACg < 2:10238/148‑1 (MQ=255) cGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACg < 1:30037/148‑1 (MQ=255) ggAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCg > 3:222658/1‑90 (MQ=255) ggAGGTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCg < 4:222658/90‑1 (MQ=255) gTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt > 8:6401/1‑145 (MQ=255) gTATAGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt < 7:6401/145‑1 (MQ=255) ataGCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACCCCAGGTTGCGGTCGCCGAACACGTTGTCgcc > 5:438106/1‑147 (MQ=255) gCCACCATCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg > 6:102889/1‑149 (MQ=255) ccaccaTCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 5:139989/1‑149 (MQ=255) caccaTCGCATTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc < 6:139989/149‑1 (MQ=255) ctttCGCGG‑GTAAACC‑CGCTCCTACACAGAGCGCGCGACCCTAGGCAGGTGGCGGGGAGGGCTATGAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc < 7:299375/147‑1 (MQ=255) aTTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTg < 6:356058/112‑1 (MQ=255) aTTCGCGG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTg > 5:356058/1‑112 (MQ=255) cgG‑GTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg < 5:102889/149‑1 (MQ=255) gg‑gTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCa > 6:442650/1‑147 (MQ=255) gg‑gTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCa < 5:442650/147‑1 (MQ=255) g‑gTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa > 4:411557/1‑149 (MQ=255) gTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc > 7:292626/1‑143 (MQ=255) gTAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 8:292626/143‑1 (MQ=255) | TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGG‑GTAAA‑C‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126498‑1126787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |