| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_002947 | 4,980,585 | +GGC | intergenic (‑24/+44) | PP_4387 ← / ← flgE | hypothetical protein/flagellar hook protein FlgE |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_002947 | = 4980585 | 1 (0.010) | 55 (0.730) +GGC |
48/272 | 0.8 | 98.2% | intergenic (‑24/+44) | PP_4387/flgE | hypothetical protein/flagellar hook protein FlgE |
| ? | NC_002947 | 4980586 = | 1 (0.010) | intergenic (‑25/+43) | PP_4387/flgE | hypothetical protein/flagellar hook protein FlgE | |||||
TAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCG > NC_002947/4980452‑4980734 | tagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGcc < 5:270099‑M2/12‑1 (MQ=255) ggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCaa > 5:285972‑M2/134‑149 (MQ=255) accaggctttgccattgatagaccggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACaaa < 5:96040‑M2/23‑1 (MQ=255) caggcttccccatcgatcgaccggggcgggcgcagcggggccaggcagaaccagcaccagcaagggcaacaaggccactaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAacac < 7:258975‑M2/26‑1 (MQ=255) atagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACaa > 5:109341‑M2/109‑148 (MQ=255) atagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACaa > 3:137057‑M2/109‑148 (MQ=255) aacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAatcat > 2:5293‑M2/105‑148 (MQ=255) ggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑c < 3:358664‑M2/50‑1 (MQ=255) gggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGGCCCAATTACAACCACCAAAGCCAATACAATCATCAGGT‑CAt > 5:24696‑M2/95‑146 (MQ=255) ggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCg > 8:231470‑M2/94‑102 (MQ=255) ggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCg < 7:231470‑M2/9‑1 (MQ=255) gcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGaa > 5:102148‑M2/93‑149 (MQ=255) cagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAat < 6:285972‑M2/58‑1 (MQ=255) agcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAatg > 4:113618‑M2/91‑149 (MQ=255) gggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGt > 8:228461‑M2/86‑149 (MQ=255) aggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTg > 3:271612‑M2/82‑148 (MQ=255) gcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCGTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCat > 7:187333‑M2/80‑149 (MQ=255) gaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgttaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAACCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCatgat < 1:384024‑M2/73‑1 (MQ=255) accagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttgcggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATgg > 5:408853‑M2/75‑149 (MQ=255) cagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCCATCTGAATGATGGTCTGCATGATGGt < 1:5293‑M2/77‑1 (MQ=255) cagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTg > 4:439252‑M2/73‑149 (MQ=255) cagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTg > 2:301597‑M2/73‑149 (MQ=255) cagcaccagcaaggggaacaaggccagtaacgacagccgcttcattagcgtgaaccttcacattcttggggcCCCTTTGCGGGCCCAATTAAAAACCCCAAAGCCAATACAAACATCAGGT‑GATGAGAATGATTGGCTTCATGATGGTg > 3:234942‑M2/73‑149 (MQ=255) gcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATAATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCt > 3:74341‑M2/71‑149 (MQ=255) accagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCttt < 1:319132‑M2/81‑1 (MQ=255) cagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCGTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTTAATGATGGTCTGCATGATGGTGCttt > 3:269789‑M2/67‑147 (MQ=255) ggggAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGGGGGCCGAAGTACAAACACAAAAGCCAAAACAATCATCAGGG‑GATGGGTAAGGTGGTCTGGAAGATGGTGGTTTCAGTGGa > 6:45282/1‑148 (MQ=255) gaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATc > 3:97294‑M2/58‑110 (MQ=255) gaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATc < 4:97294‑M2/53‑1 (MQ=255) gaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATg < 3:113618‑M2/92‑1 (MQ=255) aacaaggccagtaacgaccgccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATgg < 2:414517‑M2/93‑1 (MQ=255) caaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAatgatg > 8:70420‑M2/55‑116 (MQ=255) caaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAatgatg < 7:70420‑M2/62‑1 (MQ=255) ggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACCACCACCCAAGCCAATACAATCATCAGGG‑CATCTGAATGATGGTCTGCAAGATGGTGCTTTCAGTGGTAATGGTCTTg > 3:260677‑M2/52‑149 (MQ=255) gccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACa > 8:3841‑M2/51‑89 (MQ=255) gccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACa < 7:3841‑M2/39‑1 (MQ=255) cagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCATGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCa > 7:202083‑M2/49‑149 (MQ=255) cagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGACAAGACAATCATGAGGT‑CATCTGCATGATGGTCTGGATGATGGTGCGTTCAGTGGAAATGGTCttggga > 8:368329‑M2/49‑147 (MQ=255) cagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTgg < 6:102148‑M2/110‑1 (MQ=255) ccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCGAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAg > 5:372733‑M2/37‑149 (MQ=255) gcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGtt > 3:333551‑M2/35‑149 (MQ=255) gcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGtt > 2:245034‑M2/35‑149 (MQ=255) cattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGctc > 5:105640‑M2/31‑149 (MQ=255) attcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGctct > 2:47994‑M2/30‑149 (MQ=255) ttcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTg < 4:333551‑M2/121‑1 (MQ=255) cgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTgc < 6:105640‑M2/122‑1 (MQ=255) cgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTgc < 4:137057‑M2/122‑1 (MQ=255) gtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCGAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCt < 6:372733‑M2/126‑1 (MQ=255) cttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGAt > 8:32064‑M2/18‑147 (MQ=255) tcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAgg > 3:415011‑M2/16‑149 (MQ=255) cattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGttt < 4:271612‑M2/137‑1 (MQ=255) attcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTa > 6:187684‑M2/12‑149 (MQ=255) ttcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCGTTGATCAGGTTTAc < 4:74341‑M2/139‑1 (MQ=255) ttcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTAc < 6:109341‑M2/139‑1 (MQ=255) cttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTAcc < 7:368329‑M2/140‑1 (MQ=255) ggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCa < 3:439252‑M2/141‑1 (MQ=255) gcggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCa < 6:24696‑M2/141‑1 (MQ=255) gcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGc > 8:16218‑M2/3‑149 (MQ=255) cgcTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCg < 7:228461/149‑1 (MQ=255) | TAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGT‑CATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCG > NC_002947/4980452‑4980734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |