Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	191,125	C→T	V83M (GTG→ATG)	PP_0166 ←	HlyD family secretion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	191,125	0	C	T	100.0%	271.7 / NA	79	V83M (GTG→ATG)	PP_0166	HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/43);  total (36/43)

CAGGCGGTCAGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCACCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTCGATCAGGG  >  NC_002947/190979‑191268                                                                                                                                                   |                                                                                                                                                cAGGCGGTCAGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTAGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCt                                                                                                                                               >  6:145072/1‑149 (MQ=255) cAGGCGGTCAGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAACATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCt                                                                                                                                               >  5:5573/1‑149 (MQ=255)   ggCGGTCAGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTg                                                                                                                                             <  3:321760/149‑1 (MQ=255)    gCGGTCAGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTgg                                                                                                                                            <  5:311465/149‑1 (MQ=255)          aGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACg                                                                                                                                      <  6:12606/149‑1 (MQ=255)              tCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAAt                                                                                                                                  >  3:268903/1‑149 (MQ=255)                ggTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATg                                                                                                                                 <  8:410444/148‑1 (MQ=255)                 gTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGgc                                                                                                                               >  3:377691/1‑149 (MQ=255)                    tCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGgcctt                                                                                                                            >  4:182499/1‑149 (MQ=255)                        cAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGgccttgcct                                                                                                                        >  7:162580/1‑149 (MQ=255)                        cAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCACCTCCTTGGCGAAGATTTAGGCGAAGATGCCGCCCTCCAGGTACTGGATCTTCTGCATCTTGGAAGACGGAATGgccttgcat                                                                                                                        >  5:165394/1‑147 (MQ=255)                                gAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCcg                                                                                                                >  3:232735/1‑149 (MQ=255)                                 aGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCcgc                                                                                                               <  4:419168/149‑1 (MQ=255)                                 aGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCATCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATTGACTTGCCTTCACCCcgc                                                                                                               >  7:395352/1‑149 (MQ=255)                                   gCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGCATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCCCACcgcc                                                                                                              >  3:344832/1‑147 (MQ=255)                                        gCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACg                                                                                                                                      >  5:375785/1‑119 (MQ=255)                                        gCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACg                                                                                                                                      <  6:375785/119‑1 (MQ=255)                                         cGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTtc                                                                                                       <  7:369346/149‑1 (MQ=255)                                          gggTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTtcg                                                                                                      <  5:436543/149‑1 (MQ=255)                                           ggTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGTGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTtcgt                                                                                                     <  6:379870/149‑1 (MQ=255)                                               tCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGa                                                                                                  <  1:248316/148‑1 (MQ=255)                                                cATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGAt                                                                                                 <  6:376340/148‑1 (MQ=255)                                                cATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATg                                                                                                >  3:350453/1‑149 (MQ=255)                                                 aTCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATgg                                                                                               <  4:377691/149‑1 (MQ=255)                                                 aTCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTCCAATCACCCCGCGTAACTTCGTCGATgg                                                                                               >  8:172681/1‑149 (MQ=255)                                                 aTCCAGGCGCAGCAATGGCTGGCCCACTTAGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATTGCCTTGCCTTCACCCCGCGTAACTTCGTCGATgg                                                                                               >  2:284393/1‑149 (MQ=255)                                                   ccAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGgc                                                                                             >  8:370034/1‑149 (MQ=255)                                                         gcagcaATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTtcgtc                                                                                                    <  5:123454/136‑1 (MQ=255)                                                         gcagcaATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTtcgtc                                                                                                    >  6:123454/1‑136 (MQ=255)                                                             caATTGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTAGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAAt                                                                                                                                  <  3:277300/102‑1 (MQ=255)                                                                tggctggcCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGccc                                                                                <  3:182499/149‑1 (MQ=255)                                                                 ggctggcCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCa                                                                               >  1:281436/1‑149 (MQ=255)                                                                      gcCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACaa                                                                          <  7:370034/149‑1 (MQ=255)                                                                       cccACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAAt                                                                         >  4:18610/1‑149 (MQ=255)                                                                        ccACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTgg                                                                                   >  1:172219/1‑138 (MQ=255)                                                                        ccACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTgg                                                                                   <  2:172219/138‑1 (MQ=255)                                                                        ccACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAAt                                                                         >  5:85296/1‑148 (MQ=255)                                                                          aCTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCaa                                                                      <  7:108720/149‑1 (MQ=255)                                                                          aCTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCaa                                                                      <  1:83993/149‑1 (MQ=255)                                                                              cgacgaTCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGaa                                                                   <  1:284393/148‑1 (MQ=255)                                                                              cgacgaTCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGa                                                                    <  4:268903/147‑1 (MQ=255)                                                                               gacgaTCTTCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGgccttgcc                                                                                                                         <  5:251519/93‑1 (MQ=255)                                                                               gacgaTCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGgccttgcc                                                                                                                         >  6:251519/1‑93 (MQ=255)                                                                                 cgaTCTGCCCCTCCTTGGCGAAGATTTCGGTGTCGATGCCGGCCTCCAGGTTCTGGATCTTCTGCATCTTGGTAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACg                                                               <  4:202240/149‑1 (MQ=255)                                                                                 cgaTCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACg                                                               <  1:386487/149‑1 (MQ=255)                                                                                   aTCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTtcgt                                                                                                     >  6:177034/1‑109 (MQ=255)                                                                                   aTCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTtcgt                                                                                                     <  5:177034/109‑1 (MQ=255)                                                                                    tCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACg                                                               >  4:167370/1‑146 (MQ=255)                                                                                        cgcctgctTGGCGAAGATTTCGGCGACGATGCCGCCCTCCCGGTTCAGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCCCGTAACTTCGTCGATGCGCGCGACGCTGGCCCATACAATCAACAACACGAAGAACa                                                        <  4:344832/147‑1 (MQ=255)                                                                                        ccccTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACt                                                                                                         <  4:178336/100‑1 (MQ=255)                                                                                        ccccTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACt                                                                                                         >  3:178336/1‑100 (MQ=255)                                                                                        ccccTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAAc                                                         <  2:281436/148‑1 (MQ=255)                                                                                        ccccTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGGTCTGGATCTGCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACga                                                              <  4:25301/143‑1 (MQ=255)                                                                                         gactcctTTGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACTGAATGGCCTTCCCTTCACCCCGCGTAACTTCGTCTATGTGCGCGACGCTGTCCCATACAATCAAGAACACGAAGAACAg                                                       <  3:24672/147‑1 (MQ=255)                                                                                          cctcctTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAg                                                       >  1:396325/1‑148 (MQ=255)                                                                                              ctTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCa                                                  >  8:415412/1‑149 (MQ=255)                                                                                                   cGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGccc                                             >  7:163058/1‑149 (MQ=255)                                                                                                   cGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGccc                                             <  7:415412/149‑1 (MQ=255)                                                                                                    gAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGcccc                                            <  6:85296/149‑1 (MQ=255)                                                                                                    gAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGccc                                             >  3:415065/1‑148 (MQ=255)                                                                                                           tCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATgg                                      <  4:232735/148‑1 (MQ=255)                                                                                                            cGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGt                                     <  8:163058/148‑1 (MQ=255)                                                                                                                gacgaTGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGcccc                                            >  5:348583/1‑137 (MQ=255)                                                                                                                gacgaTGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGcccc                                            <  6:348583/137‑1 (MQ=255)                                                                                                                     tGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGCTGGTCAGCCGcac                            >  3:58474/1‑148 (MQ=255)                                                                                                                       ccgccCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGcacca                          <  4:415065/148‑1 (MQ=255)                                                                                                                        cgccCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAg                                                                     >  2:67667/1‑104 (MQ=255)                                                                                                                        cgccCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAg                                                                     <  1:67667/104‑1 (MQ=255)                                                                                                                        cgccCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAgg                                                      <  2:113156/119‑1 (MQ=255)                                                                                                                        cgccCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAgg                                                      >  1:113156/1‑119 (MQ=255)                                                                                                                        cgccCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCAcg                        >  3:137515/1‑149 (MQ=255)                                                                                                                                     cTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAAGTTCGTCGTTGGGCGCGACGCTGGCCCATACAATCAAGTACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCAtgt            <  6:292765/148‑3 (MQ=255)                                                                                                                                        gATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTc          <  4:350453/147‑1 (MQ=255)                                                                                                                                        gATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTc          <  5:145072/147‑1 (MQ=255)                                                                                                                                         aTCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGccc                                             <  2:315670/111‑1 (MQ=255)                                                                                                                                         aTCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGccc                                             >  1:315670/1‑111 (MQ=255)                                                                                                                                          tcttctGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGc                                               <  6:42622/108‑1 (MQ=255)                                                                                                                                             tctGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTCGATCAgg   >  1:157118/1‑149 (MQ=255)                                                                                                                                              ctGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTCGATCAggg  >  6:449420/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                CAGGCGGTCAGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCACCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTCGATCAGGG  >  NC_002947/190979‑191268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑