Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,921,460	G→A	G3494R (GGG→AGG)	PP_2561 →	hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,921,460	0	G	A	100.0%	178.5 / NA	52	G3494R (GGG→AGG)	PP_2561	hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/20);  total (32/20)

GTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATTT  >  NC_002947/2921318‑2921605                                                                                                                                               |                                                                                                                                                   gTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGAGATGACAGCCTCGATGTCGGCAAATGCAATGAAAACCTTAAGGGAGGCGATGGTAACGACACATTGTTCGGCTACGTTGGTACCGCCACCCTGATCagggggg                                                                                                                                              >  6:235787/1‑149 (MQ=255)      tGGGGGAGGCCGGTAACGACACCCTGAACGGGGGCGCTGGCGATGACCGCCTGGAGGGCGGCGACGGCAATGACACCGTGCAGGGCGGCGATGGGGAGGACACTTTGTTCGGGGCCGTGGGGAGGGTCGCCGTGAGCAGGGGGGCCggg                                                                                                                                         >  8:385741/1‑148 (MQ=255)      tGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACACTGATCAGGGGGGCCgg                                                                                                                                          >  1:15113/1‑148 (MQ=255)       gggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGTTACCGACACCCTGATCAGGGGGGCCGG‑Ca                                                                                                                                       >  8:301283/1‑149 (MQ=255)           cacgCCGGAAACGACAACCCGCCCGGGTGCCCTGGCGATGACAGCCTGGATGGCGGCACCGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGa                                                                                                                                   <  3:316309/146‑1 (MQ=255)               ccGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTc                                                                                                                               >  8:303383/1‑149 (MQ=255)                 ggTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGa                                                                                                                                   <  4:289466/143‑1 (MQ=255)                 ggTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGa                                                                                                                                   >  3:289466/1‑143 (MQ=255)                  gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCAGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCt                                                                                                                             >  8:326362/1‑148 (MQ=255)                    aaCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCATGATCAGGGGGGCCGG‑CAACGACTTCCTCaa                                                                                                                          >  3:148027/1‑149 (MQ=255)                            ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCgg                                                                                                                  <  1:385365/149‑1 (MQ=255)                            ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCgg                                                                                                                  >  2:385365/1‑149 (MQ=255)                              tGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCgg                                                                                                                  >  4:173204/1‑147 (MQ=255)                               gAACGGGGGCGCTGGCGATGACAGCCTGGATGGCTGCAACGGCAATGACACCCTGCAGGGCTGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAg                                                                                                               <  5:235787/149‑1 (MQ=255)                               gAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAg                                                                                                               >  4:354634/1‑149 (MQ=255)                                     gggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACg                                                                                                         <  7:301283/149‑1 (MQ=255)                                      ggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGa                                                                                                                                   <  1:112136/122‑1 (MQ=255)                                      ggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGa                                                                                                                                   >  2:112136/1‑122 (MQ=255)                                        cgcTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATa                                                                                                      <  4:148027/149‑1 (MQ=255)                                        cgcTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATa                                                                                                      <  7:303383/149‑1 (MQ=255)                                           tGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACgg                                                                                                   >  3:256479/1‑149 (MQ=255)                                             gCGATGACAGCCTGGATGGCAGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACgg                                                                                                   <  7:326362/147‑1 (MQ=255)                                                 ttaCAGCCTGGATGGCGTCAACGGCAATGACACCATGCAGGGCGGCGTTGGTTACGACACATTGGTCGGCGTCGTGGGTACCGACACGGTGATGAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGtggtgg                                                                                             <  5:241861/147‑1 (MQ=255)                                                    cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg                                                                                          <  6:249248/149‑1 (MQ=255)                                                     aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGgtgggtgg                                                                                         >  8:322422/1‑149 (MQ=255)                                                          ggATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCg                                                                                    >  3:344161/1‑149 (MQ=255)                                                           gATGGAGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTATCGAGACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCgg                                                                                   <  4:256479/149‑1 (MQ=255)                                                                cggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGTCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAAc                                                                               >  3:55443/1‑148 (MQ=255)                                                                         caaTGACACCCTGCAGGGCGGCGAAGGTAACGACACATTGTTCGGCGACGTGGTTACCGAAACCCTGATCAGTGGGGCCGG‑CAACGAATTCATCAACGGTGCGGCAGGTAAACATACGGTGGTGGGTGGGGCAGGTAACGACACCATg                                                                      >  8:106423/1‑148 (MQ=255)                                                                                acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAc                                                              >  1:83890/1‑149 (MQ=255)                                                                                acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAc                                                              >  8:349505/1‑149 (MQ=255)                                                                                  ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc                                                             >  5:43954/1‑148 (MQ=255)                                                                                  ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc                                                             >  6:373025/1‑148 (MQ=255)                                                                                            ggcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa                                                  <  6:43954/149‑1 (MQ=255)                                                                                            ggcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa                                                  >  3:115116/1‑149 (MQ=255)                                                                                                   gTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                           >  2:259741/1‑149 (MQ=255)                                                                                                        gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                           >  8:36405/1‑144 (MQ=255)                                                                                                        gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                           <  7:36405/144‑1 (MQ=255)                                                                                                        gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGt                                       >  4:40904/1‑148 (MQ=255)                                                                                                        gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGt                                       >  4:4180/1‑148 (MQ=255)                                                                                                          cacaTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCg                                    <  7:349505/149‑1 (MQ=255)                                                                                                             aTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGTGGGGCCGG‑CAACGACTACCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCCATGGAAACGATGTGTTCCAGTTCGCTg                                 >  2:340132/1‑149 (MQ=255)                                                                                                             aTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAAAGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCCCTg                                 >  4:403468/1‑149 (MQ=255)                                                                                                               tGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGACAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                                 >  1:310826/1‑148 (MQ=255)                                                                                                                gTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATATGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCa                               <  3:354634/148‑1 (MQ=255)                                                                                                                    ggCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCtt                          >  3:426686/1‑149 (MQ=255)                                                                                                                       gACGTTGGTAACGTAATCCTGATCAGGGGGGCCGG‑CAACGACTTCCTAAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGGAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTgg                       <  7:106423/149‑1 (MQ=255)                                                                                                                             ggTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGGGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa                                                  <  2:62562/116‑1 (MQ=255)                                                                                                                                aCCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCt              >  3:199323/1‑149 (MQ=255)                                                                                                                                        ccTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCtt                          <  3:167999/129‑1 (MQ=255)                                                                                                                                        ccTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCtt                          >  4:167999/1‑129 (MQ=255)                                                                                                                                            aTCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAttt  >  3:377652/1‑149 (MQ=255)                                                                                                                                            aTCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAttt  <  2:83890/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   GTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATTT  >  NC_002947/2921318‑2921605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑