Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,816 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8160TG98.2% 187.6 / ‑6.3 57intergenic (+53/+700)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base G (32/23);  minor base C (0/1);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCAC  >  NC_002947/4740679‑4740952
                                                                                                                                          |                                                                                                                                         
cAGCCCGTACAAGCTCGGCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                               <  2:73512/149‑1 (MQ=255)
cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTATCACCGGCCAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGGTGAAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTTTCCAGTGCCgg                                                                                                                                >  1:73512/1‑149 (MQ=255)
        aCAAGATCGGCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                       >  4:363185/1‑149 (MQ=255)
         cAAGATCGGCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgcg                                                                                                                      <  4:364044/149‑1 (MQ=255)
         cAAGATCGGCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                       <  7:61845/148‑1 (MQ=255)
            gATCGGCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGGCGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                                   >  5:320884/1‑149 (MQ=255)
            gATCGGCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                                   >  6:261341/1‑149 (MQ=255)
               cGGCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTg                                                                                                               >  5:218232/1‑149 (MQ=255)
                 gCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGcc                                                                                                             >  5:450015/1‑149 (MQ=255)
                 gCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGcc                                                                                                             >  2:2722/1‑149 (MQ=255)
                  ccGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGccc                                                                                                            <  2:247018/149‑1 (MQ=255)
                      ccGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGc                                                                                                              <  6:431389/143‑1 (MQ=255)
                      ccGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGc                                                                                                              >  5:431389/1‑143 (MQ=255)
                       cgcgCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGaa                                                                                                       <  3:363185/149‑1 (MQ=255)
                        gcgcCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAag                                                                                                      <  7:117575/149‑1 (MQ=255)
                          gcCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTAATCGCGGCC‑GTGCCCGCGAagag                                                                                                    >  3:247378/1‑149 (MQ=255)
                              gCTGTA‑CACCGGCGAGCAGCAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCg                                                                                                <  5:255387/149‑1 (MQ=255)
                              gCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGGCCGCGAAGAGGCCg                                                                                                >  1:193107/1‑149 (MQ=255)
                                  tA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGc                                                                                              <  6:218232/147‑1 (MQ=255)
                                        cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGgacagaca                                                                                       >  5:374145/1‑149 (MQ=255)
                                        cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACAGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTAGTCCTGTGCCGGCCTCTTCCCGGCC‑TTGCCCGCGAAGACGGCCGCACAGaca                                                                                       >  5:337576/1‑149 (MQ=255)
                                                 gAAGCGCGACATCGTTGCCCTGAAAGACCGCAATTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                              <  6:443261/149‑1 (MQ=255)
                                                 gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACCCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                              <  5:194602/149‑1 (MQ=255)
                                                  aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAag                                                                                                      <  1:182659/124‑1 (MQ=255)
                                                  aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAag                                                                                                      >  2:182659/1‑124 (MQ=255)
                                                         aCATCGTTGCCCTGAAAGCCCGCAAGTAAGCTGCATTAGCCGAACGCAATAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGttt                                                                      <  4:345953/149‑1 (MQ=255)
                                                           aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGtttt                                                                     >  2:58867/1‑148 (MQ=255)
                                                             cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAAGAGGcc                                                                                                 >  6:225582/1‑118 (MQ=25)
                                                             cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAAGAGGcc                                                                                                 <  5:225582/118‑1 (MQ=25)
                                                                  cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGAcaccac                                                                                   <  1:262088/127‑1 (MQ=39)
                                                                  cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGAcaccac                                                                                   >  2:262088/1‑127 (MQ=39)
                                                                   ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAcc                                                                             >  1:289325/1‑132 (MQ=255)
                                                                   ccTCAAACACCGAAAGAAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCTAAGAGGCCGGCACAGACACCACAAAAcc                                                                             <  2:289325/132‑1 (MQ=255)
                                                                     tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGGCGATTCGGGGCCGTTGCCC‑CGCAGAGGGCGGCACAGACACCACAAAACCGGAGGTTTTATacgcccgc                                                          >  3:394552/1‑149 (MQ=255)
                                                                      gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACATAGCGGCC‑ATGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCGCAGTTTTTGTacgcccgcg                                                         >  1:404053/1‑148 (MQ=255)
                                                                             cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAg                                                  >  2:5137/1‑149 (MQ=255)
                                                                             cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAg                                                  >  7:198617/1‑149 (MQ=255)
                                                                               cAATTAAGCTGCCGTACCCGAACGCAAGACGGCTGCCCTGGGGAAG‑CGTTTTTTATTTGCCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                            <  8:413828/137‑1 (MQ=255)
                                                                               cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGCAGAGGCCGGCCCAGACACCACAAAACCACCCTTTTTCTacgccc                                                            >  7:413828/1‑137 (MQ=255)
                                                                                         gCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAggg                                       >  2:291543/1‑148 (MQ=255)
                                                                                          cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCGCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                                     >  1:147658/1‑149 (MQ=255)
                                                                                           aGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑GTGCCCGCGAAGAGGCCGGCACAGACCCCAGAAAACCTCAGTTTTTATCCCCCCGCGGACGCCGCCCGTTGAggggtt                                    >  8:183909/1‑146 (MQ=255)
                                                                                            gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                                   <  1:2722/149‑1 (MQ=255)
                                                                                               gCCGACCGCAAGACGGCGGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTCCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGa                                 <  8:198617/148‑1 (MQ=255)
                                                                                               gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGaa                                >  2:306574/1‑149 (MQ=255)
                                                                                                ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc                               >  6:222836/1‑149 (MQ=255)
                                                                                                ccGAACGCAAGAAGGCTGCCCTCAGGAAGCC‑TTTTTTATTTGTCATGTACCGGCCAATTAGCGGCC‑TTACCCGCGAAAAGGCCGGCACAGACACCACAAAACCAAAATTTTTCTACCCCCGCTCACGCAGCCCTTTAAGGGTTTTGAAc                               >  7:279213/1‑149 (MQ=255)
                                                                                                  gAACGCAAGAAGGCTGCCCTCGGGCAG‑CGTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACgg                             >  3:320255/1‑149 (MQ=255)
                                                                                                  caaCGCAAGAAGGGGGCCCTGGGGCAG‑CTTTTTTTATTTCCCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACgg                             <  5:261341/148‑1 (MQ=255)
                                                                                                        aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                       >  2:70587/1‑149 (MQ=255)
                                                                                                        aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                       <  1:58867/149‑1 (MQ=255)
                                                                                                            agGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAGAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                    >  7:234430/1‑148 (MQ=255)
                                                                                                              gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCcaccac                 >  6:220975/1‑149 (MQ=255)
                                                                                                                       gggCAGGC‑TTTTTTATTTGTCCTGGGCCGGCCTCTTCGGCGCC‑TTGCCCGCGAAGAGGCAGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt        <  6:320884/149‑1 (MQ=255)
                                                                                                                       gggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTTAGGGTATTGAACGGCGCATCCACCACGAACttgtt        <  1:291543/149‑1 (MQ=255)
                                                                                                                       gggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttg          <  5:222836/147‑1 (MQ=255)
                                                                                                                              ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccac  >  3:112320/3‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                         
CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTA‑CACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCAC  >  NC_002947/4740679‑4740952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑