Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,621,706	G→A	L194F (CTT→TTT)	PP_4940 ←	glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,621,706	0	G	A	97.4%	254.4 / ‑2.6	79	L194F (CTT→TTT)	PP_4940	glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (37/39);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

GCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAGCGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGATATCACGCTCA  >  NC_002947/5621568‑5621848                                                                                                                                           |                                                                                                                                                 gCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATTGCTTCGAAGTTCTTGATCATGTATTCGCCCATTCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTg                                                                                                                                           <  3:374045/146‑1 (MQ=255) gCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTg                                                                                                                                           <  1:124772/146‑1 (MQ=255) gCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTg                                                                                                                                           <  3:76171/146‑1 (MQ=255)     aCCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCagtagt                                                                                                                                    >  8:289075/1‑149 (MQ=255)     aCCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCagtagt                                                                                                                                    >  2:233192/1‑149 (MQ=255)       ccAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCagtag                                                                                                                                     <  3:326393/146‑1 (MQ=255)       ccAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCagtag                                                                                                                                     >  4:326393/1‑146 (MQ=255)       ccAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTc                                                                                                                                   >  1:179765/1‑148 (MQ=255)       ccAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTc                                                                                                                                   <  2:179765/148‑1 (MQ=255)                ggTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACgt                                                                                                                                               <  8:146497/127‑1 (MQ=255)                ggTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGAc                                                                                                                         <  3:424975/149‑1 (MQ=255)                  ttCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACtt                                                                                                                       >  8:84528/1‑149 (MQ=255)                       ttCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCagta                                                                                                                                      <  6:385264/129‑1 (MQ=255)                       ttCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCagta                                                                                                                                      >  5:385264/1‑129 (MQ=255)                       ttCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCa                                                                                                                                         >  7:114555/1‑126 (MQ=255)                       ttCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCa                                                                                                                                         <  8:114555/126‑1 (MQ=255)                       ttCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTAAAACGTGTTGCAGTAGTCCTCGCCCTACTTGGTAc                                                                                                                  >  5:256941/1‑149 (MQ=255)                             gCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑Gt                                                                                                            <  7:70164/147‑1 (MQ=255)                                   gTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAAc                                                                                                     <  1:233192/148‑1 (MQ=255)                                    tCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACa                                                                                                    <  7:289075/148‑1 (MQ=255)                                    tCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAg                                                                                                   <  7:270114/149‑1 (MQ=255)                                     cGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCag                                                                                                                                        <  2:297155/113‑1 (MQ=255)                                     cGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCag                                                                                                                                        >  1:297155/1‑113 (MQ=255)                                       tATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGtt                                                                                                >  4:232105/1‑149 (MQ=255)                                        aTGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑Gt                                                                                                            <  7:363024/136‑1 (MQ=255)                                        aTGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑Gt                                                                                                            >  8:363024/1‑136 (MQ=255)                                          gCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATATGGTTAAACGTGTTg                                                                                                                                           <  4:440440/105‑1 (MQ=255)                                          gCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATATGGTTAAACGTGTTg                                                                                                                                           >  3:440440/1‑105 (MQ=255)                                          gCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTAAAACGTGTTGAAGAAGTCATAGCCCGACTTGGTACGG‑‑CTTACAAACAGGtaat                                                                                              >  2:388343/1‑145 (MQ=255)                                            cAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGa                                                                                           >  1:42202/1‑149 (MQ=255)                                              gCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCACGACTTGGTAAGT‑‑CTTACAAACAGGTTCTCGAcc                                                                                         >  1:295796/1‑149 (MQ=255)                                                 acacacAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGc                                                                                      >  2:238223/1‑149 (MQ=255)                                                 acacacAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGc                                                                                      >  7:164704/1‑149 (MQ=255)                                                    cacaGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGccc                                                                                    >  2:318571/1‑148 (MQ=255)                                                     acaGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGc                                                                                                                              <  4:206221/107‑1 (MQ=255)                                                     acaGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGc                                                                                                                              >  3:206221/1‑107 (MQ=255)                                                      caGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCaa                                                                                 <  1:388343/149‑1 (MQ=255)                                                       aGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAAc                                                                                >  7:172751/1‑149 (MQ=255)                                                         ccACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGGCATCGGCACTGACGAAGAAACGAATACGGTTAAACgtgt                                                                                                                                             <  8:379066/88‑1 (MQ=255)                                                         ccACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTc                                                                              <  8:164704/149‑1 (MQ=255)                                                         ccACAGGCCATGGCGTCGAAGTTCTTGATCATGTATTCGCCCATGCCGGCATCGGCACTGACGAAGAAACGAATACGGTTAAACgtgt                                                                                                                                             >  7:379066/1‑88 (MQ=255)                                                           acaGGCCATGGCTTCGAAGTTCTTGATGATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATAGGGGTAATCGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGt                                                                            <  4:318409/149‑1 (MQ=255)                                                                   tGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGt                                                                    >  7:95531/1‑149 (MQ=255)                                                                          gAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGt                                                                            <  5:161127/134‑1 (MQ=255)                                                                          gAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGt                                                                            >  6:161127/1‑134 (MQ=255)                                                                          gAAGTTCTTGATCATGTATTCGCCCATGCCAACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGc                                                             >  3:73423/1‑149 (MQ=255)                                                                                    aTCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGccc                                                                                    >  1:122510/1‑116 (MQ=255)                                                                                    aTCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGccc                                                                                    <  2:122510/116‑1 (MQ=255)                                                                                     tCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAGCGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGc                                                             <  2:330104/138‑1 (MQ=255)                                                                                     tCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAGCGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGc                                                             >  1:330104/1‑138 (MQ=255)                                                                                          tATTAGCCCATTCCGAAATCGGCACTTACCAAGAAACGTATACGGTTAAACGTGTTTCAGTATGCCTCGCCCTAATTGGTACGGTCGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGc                                                <  1:350884/148‑1 (MQ=255)                                                                                            ttCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCacac                                           >  2:427442/1‑149 (MQ=255)                                                                                            ttCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCacac                                           >  2:442951/1‑149 (MQ=255)                                                                                             tCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAg                                                  <  2:6854/141‑1 (MQ=255)                                                                                             tCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAg                                                  >  1:6854/1‑141 (MQ=255)                                                                                                cccATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGtt                                       <  6:27368/149‑1 (MQ=255)                                                                                                cccATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGtt                                       <  1:442951/149‑1 (MQ=255)                                                                                                  cATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGa                                     >  2:329245/1‑149 (MQ=255)                                                                                                       cGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGa                                     >  8:220656/1‑144 (MQ=255)                                                                                                       cGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGa                                     <  7:220656/144‑1 (MQ=255)                                                                                                       cGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTg                                >  5:10398/1‑149 (MQ=255)                                                                                                        gACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGc                               <  7:84528/149‑1 (MQ=255)                                                                                                           aTCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTgg                                                                                                                     >  2:113059/1‑62 (MQ=255)                                                                                                           aTCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTgg                                                                                                                     <  1:113059/62‑1 (MQ=255)                                                                                                            tCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCtt                                                               <  2:268384/113‑1 (MQ=255)                                                                                                            tCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCtt                                                               >  1:268384/1‑113 (MQ=255)                                                                                                                cACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAg                       <  8:172751/149‑1 (MQ=255)                                                                                                                cACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAg                       <  3:232105/149‑1 (MQ=255)                                                                                                                  cTGACGAAGAAACGAATACGGTTAAACGGGGGGCAGTAGTCCTCGCCCGCCTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGc                     <  6:256941/149‑1 (MQ=255)                                                                                                                       gaagaaACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGtt                >  5:322997/1‑149 (MQ=255)                                                                                                                         agaaACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCcaca                                            <  8:145086/119‑1 (MQ=255)                                                                                                                         agaaACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCcaca                                            >  7:145086/1‑119 (MQ=255)                                                                                                                         agaaACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTc               >  7:274110/1‑148 (MQ=255)                                                                                                                          gaaACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGa             <  6:322997/149‑1 (MQ=255)                                                                                                                          gaaACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGa             <  1:318571/149‑1 (MQ=255)                                                                                                                            aaCGAATACGGTTAAACGTGTTGCAGTATTCCACGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGat            <  2:295796/148‑1 (MQ=255)                                                                                                                             aCGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGatat          <  1:427442/149‑1 (MQ=255)                                                                                                                               gAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGATAtca        <  6:10398/149‑1 (MQ=255)                                                                                                                                     ggTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGATAtcacgctca  >  6:165215/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                                 GCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAGCGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGG‑‑GTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGATATCACGCTCA  >  NC_002947/5621568‑5621848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑