Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,753	G→C	intergenic (+115/+25)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,753	0	G	C	98.6%	237.7 / NA	69	intergenic (+115/+25)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (28/40);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGC‑GCG‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674613‑5674890                                                                                                                                               |                                                                                                                                           cAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCgg                                                                                                                                    <  8:278641/149‑1 (MQ=255)  aGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGt                                                                                                                                   >  6:450579/1‑149 (MQ=255)      ctgctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTc                                                                                                                               <  5:88675/149‑1 (MQ=255)         ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCa                                                                                                                              <  4:201295/147‑1 (MQ=255)         ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAg                                                                                                                             <  3:182813/148‑1 (MQ=255)            aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCGTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTgg                                                                                                                         <  1:434179/149‑1 (MQ=255)             cccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCGCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTgg                                                                                                                         >  7:233346/1‑148 (MQ=255)              ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTgg                                                                                                                         >  5:212179/1‑147 (MQ=255)                 cAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGaa                                                                                                                    <  3:78322/149‑1 (MQ=255)                 cAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGaa                                                                                                                    <  3:355431/149‑1 (MQ=255)                  aaGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGACCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGaaa                                                                                                                   >  8:251006/1‑149 (MQ=255)                          aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCCGTGATATGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACt                                                                                                              <  1:109255/147‑1 (MQ=37)                              aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                       <  3:216208/149‑1 (MQ=255)                              aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                       <  4:376305/149‑1 (MQ=255)                               aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                       <  6:327493/148‑1 (MQ=255)                                    cGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGGTAGGGCCGCAAAGCGGCC‑CCCGCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                                 >  8:62549/1‑149 (MQ=38)                                    cGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                                 >  7:409387/1‑149 (MQ=255)                                       gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              <  5:450579/149‑1 (MQ=255)                                       gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              <  8:409387/149‑1 (MQ=255)                                         gggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCg                                                                                             <  3:192139/148‑1 (MQ=255)                                         gggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCg                                                                                             >  4:192139/1‑148 (MQ=255)                                          ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCGCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                            >  1:440137/1‑148 (MQ=255)                                          ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGAGGCCTTGTTT‑CGTGATAGGGCCGCAAAGC‑GGGCCCCTCGGTTTTCAGCTGGAAGAAATTACTTATGCTTaacaacatata                                                                                            >  6:312302/1‑141 (MQ=37)                                           gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGAC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca                                                                                          >  4:247133/1‑149 (MQ=255)                                               ctTCGCAGCCCTATCACGACTCAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                       <  3:358588/148‑1 (MQ=255)                                               ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcac                                                                                                    >  6:194178/1‑135 (MQ=255)                                               ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcac                                                                                                    <  5:194178/135‑1 (MQ=255)                                               ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACt                                                                                        <  4:243243/147‑1 (MQ=255)                                               ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                       <  5:309434/148‑1 (MQ=255)                                                      gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                               <  1:219449/149‑1 (MQ=255)                                                       cccTATCACGACACAAGGCCGCTGCTGCAGAGAATTTCTTTATCTCTACAGTAGCGGCCTTGTGT‑CGTGATATGGCCTCAAAGCTGCC‑CCCGAGTTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccagg                                                                              <  5:312302/149‑1 (MQ=37)                                                            tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccag                                                                         >  4:163229/1‑149 (MQ=255)                                                            tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccag                                                                         >  8:320238/1‑149 (MQ=255)                                                              aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGaa                                                                                                                    <  4:280715/104‑1 (MQ=255)                                                              aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGaa                                                                                                                    >  3:280715/1‑104 (MQ=255)                                                                 acacAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCt                                                                    <  1:388754/149‑1 (MQ=255)                                                                 acacAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGACCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCt                                                                    <  7:251006/149‑1 (MQ=255)                                                                  cacaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTg                                                                   <  6:212179/149‑1 (MQ=255)                                                                     aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg                                                                 >  3:149565/1‑148 (MQ=255)                                                                       ggCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAg                                                              >  1:48208/1‑149 (MQ=255)                                                                       ggCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAg                                                              <  7:320238/149‑1 (MQ=255)                                                                        gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              >  5:139024/1‑116 (MQ=255)                                                                        gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              <  6:139024/116‑1 (MQ=255)                                                                        gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAg                                                              >  1:275856/1‑148 (MQ=255)                                                                         ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                            <  2:413221/149‑1 (MQ=255)                                                                           gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                           >  1:281883/1‑148 (MQ=255)                                                                            cTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAACCAGGTCCAGGGTCTTAGAAGTAGCa                                                         >  3:348820/1‑149 (MQ=38)                                                                               cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccagg                                                                              <  3:314384/125‑1 (MQ=255)                                                                               cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccagg                                                                              >  4:314384/1‑125 (MQ=255)                                                                                  cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGt                                                             <  3:345644/139‑1 (MQ=255)                                                                                  cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGt                                                             >  4:345644/1‑139 (MQ=255)                                                                                   agagAATTTCTTGATCTCTACAGGAGCGGCTTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGc                                                  >  1:40614/1‑149 (MQ=255)                                                                                      gaATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCtt                                               <  8:233346/149‑1 (MQ=255)                                                                                        aTTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                              <  4:149565/148‑1 (MQ=255)                                                                                           tCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGGTAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                           <  7:62549/148‑1 (MQ=255)                                                                                           tCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                           <  3:247133/148‑1 (MQ=255)                                                                                                     tACCGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                     <  4:187172/144‑1 (MQ=255)                                                                                                       cAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCTCACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                               <  7:409724/148‑1 (MQ=255)                                                                                                       cAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCTCACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                               <  7:415380/148‑1 (MQ=255)                                                                                                        aGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                     <  7:137476/141‑1 (MQ=255)                                                                                                        aGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                     >  8:137476/1‑141 (MQ=255)                                                                                                         ggAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                            <  2:48208/149‑1 (MQ=255)                                                                                                                    gtgt‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtgg                 >  3:234844/1‑149 (MQ=255)                                                                                                                    gtgt‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGAAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtgg                 >  1:98371/1‑149 (MQ=255)                                                                                                                       t‑CGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg              >  3:314953/1‑149 (MQ=255)                                                                                                                           tGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg           >  6:260059/1‑149 (MQ=255)                                                                                                                           tGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg           <  5:260059/149‑1 (MQ=255)                                                                                                                                gggCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      <  7:139493/149‑1 (MQ=255)                                                                                                                                    cGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  8:132191/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                           CAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGT‑CGTGATAGGGCCGCAAAGC‑GCG‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674613‑5674890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑