| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 93.5% | 124.2 / ‑5.8 | 46 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base A (18/25); total (20/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 129.0 / NA | 46 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (20/26); total (20/26) | |||||||||||
CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAT > NC_002947/1499339‑1499616 || cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCtt > 4:108485/1‑149 (MQ=255) aCGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCCTGCGAAACAGGCGCCGCGGTGCATGGCACCGGCttt < 1:292550/149‑1 (MQ=255) aCGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg < 2:200007/149‑1 (MQ=255) cGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgg > 7:77843/1‑144 (MQ=255) gACAACGCTGAAGCCCGTTATGAGGCCATTCAGAGCCTGAACGAAGGCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgg < 8:317121/148‑6 (MQ=37) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgg < 3:330503/148‑6 (MQ=255) aCAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgggc < 3:429119/149‑8 (MQ=255) aCAACGCTGAAGCCCGTGATGCGACCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgggc < 1:285080/149‑8 (MQ=37) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCAcg‑cccg < 1:403737/147‑3 (MQ=25) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGAGGGCAcg‑cccgct > 4:283856/1‑145 (MQ=25) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCca > 5:100936/1‑148 (MQ=25) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCCTGCCC‑GCGCAACAGGCGCCCCGGTGCATGGCCCCGGCGTTTCCCGTGT‑TAGCGGGGCCGCCCCCTCCCACAGa > 6:384915/1‑147 (MQ=255) ggCCTTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑CCGAAACAGGCCCCGCGGTGCATGGCACCGGCTTTTCCGGTGT‑TCCCGGGCACGCCCGCTCCCACAGACAGAt > 6:347042/1‑149 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACACGCGCCCCGGTGCATGGCACCGGCGTTTCCGGGGT‑TAGCGGGCCCGCCCGCACCCCCACACAGATTGc > 2:84465/1‑148 (MQ=21) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGc < 1:84465/149‑1 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTACATGGCACCGGCTTTGCCGGTGT‑TCGCCGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCggg > 8:419339/1‑149 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGT‑TAGCGGGCACGCCCGCGGCCACAGACTGATTGGCATTAAGCGGGc > 3:367155/1‑149 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 8:77843/148‑1 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 5:384915/148‑1 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt > 5:218489/1‑149 (MQ=21) cacaGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg > 5:330893/1‑149 (MQ=21) gcagGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc < 3:108485/149‑1 (MQ=21) gACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg > 6:353844/1‑122 (MQ=18) gACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 5:353844/122‑1 (MQ=18) gACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCCGTGCATTGCACCCGCTTTTCCGGTGT‑TAGCGGGCACGCCCGCACCCACAGACAGAATGCCATTAAGCCGGCCGATCGCTAAAGTGACGGCCCGgtt > 8:87067/1‑149 (MQ=18) aCTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACACGCGCCCCGGTGCATGGCACCCGCTTTTCCGGTGT‑TAGCGGGCACGCCCCCTCCCACAAACATATTGCCATTAAGCGGGCCGAAAGCTACAGTGACCGCCCGtttt > 4:377554/1‑149 (MQ=18) cTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt < 7:374951/149‑1 (MQ=21) cgccgCCAAACGGGTGGTCGGGGGGGGGCCC‑GCTAAACTGGCGCCGGGGTGCATGGCCCGGGCTTTGCGGTTGTGG‑GGGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 6:18399/145‑1 (MQ=255) cTGCCAAAC‑GGTGGTAGGGGGCGTGCCG‑GCGAAACTGGGCCCGCGGTCCATGGCACGGGCTTTGCCGGTGT‑TCGGGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc < 5:347042/149‑1 (MQ=255) aGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCCTGGCCCCGGCTTTGCCGGTGT‑TAGCGGGCCCGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCGATCGCGACCGGGGCGGCCCGGTTTTTGTTGACTCGCGAACGAATCa > 2:364303/1‑149 (MQ=18) aGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGGTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 5:382313/1‑149 (MQ=25) gCGGGCGTGCCC‑GCGAAACAGGCGGCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGTCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:333074/149‑1 (MQ=21) gCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 8:131643/149‑1 (MQ=25) gCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 6:218489/149‑1 (MQ=25) cGGGCGTGCCC‑GCGAAACAGGCGCCGCGTTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATg < 7:87067/149‑1 (MQ=25) gggCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 7:276473/149‑1 (MQ=37) cc‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 5:244348/1‑147 (MQ=37) cc‑GCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 6:100936/149‑1 (MQ=37) c‑gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt < 2:5583/147‑1 (MQ=37) c‑gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt > 1:5583/1‑147 (MQ=37) gccgcGGTACATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 7:419339/149‑1 (MQ=37) ccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 6:79091/149‑1 (MQ=37) cgcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 2:64660/1‑147 (MQ=37) ggTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt < 8:194841/149‑1 (MQ=38) cATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa < 5:248144/130‑1 (MQ=37) cATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa > 6:248144/1‑130 (MQ=37) || CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAC‑GGTGGGAGCGGGCGTGCCC‑GCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAT > NC_002947/1499339‑1499616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |