| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 97.7% | 141.9 / ‑3.5 | 43 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (25/17); total (26/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGA > NC_002947/1932501‑1932768 | ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAggg > 4:244390/1‑149 (MQ=255)ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAggg > 3:44171/1‑149 (MQ=255) gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTTTAGGAGCGGGCTTGCC‑CGCGAAGGGCTg > 1:13108/1‑149 (MQ=255) gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGc > 1:253450/1‑148 (MQ=255) tCTGGACATTCGACAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAg > 7:89894/1‑149 (MQ=255) gACATTCGACAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCCAGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGccc > 1:97411/1‑149 (MQ=255) gACATTCGACAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGcc < 1:45398/147‑1 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTACTACAAAGGATAAGCGTACCCTGTAAGACCGGGCATGCC‑CGCGAACGGCTTCAAGGCAGcagatac > 3:253553/1‑143 (MQ=37) ttCGACAGCGGCATTGCAGGTCTCACT‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTCGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCGGCCCCAAAt < 2:253450/149‑1 (MQ=37) aCAGCGGCATTGCAGGTCTCACG‑AAAATAGCATTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 4:44171/149‑1 (MQ=255) aCAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGTCAAAGGATATTCGTCCCCGGTTGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc > 7:128776/1‑149 (MQ=37) ctgCGGCTTGGCTGTTCTCACT‑AAAATAGCATTAAACGGGGGGGCC‑GTTTTGGGGCCCTTCGGGGGAAAGCCGGCTCCAACAATGTATATGGTTCCCCTTTTGTTGCGGGGTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 8:32701/146‑1 (MQ=255) gCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTTTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgcg < 5:248806/147‑1 (MQ=255) gCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgcg > 6:248806/1‑147 (MQ=255) aGGTTTCACC‑AAAATAGCATTAAACGGTGGGGCC‑GGTTTGGGGCCCTTCGGGGGCAAGCCCGCTCCTAAAAAGGTTATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt < 2:13108/148‑1 (MQ=37) tctaACT‑AAAATAGAATTAAACGCTGGGGCC‑GCTTTGCGGCCGTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGc < 8:314178/149‑1 (MQ=38) aCG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAt < 3:270890/148‑1 (MQ=255) cccgTAACCGCGGGGGCC‑GCTTGGCGGCCCTGCGCGGGCACGCCCGCTCCTACAACGGATATGCGGCCCCTGTCGGCGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGt < 3:244390/146‑1 (MQ=37) aaCGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt > 7:58910/1‑149 (MQ=255) aaCGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt > 7:176368/1‑149 (MQ=255) aaCGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCCCGCCGACAAAGGATATGCGTCCCCAGTAGGAGCCGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACAAAGCGGCTCAGCGATCCGGCAGGGTGATGt > 2:285462/1‑148 (MQ=37) tGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGGCCCCTGTAGCAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGCTGATGTTCCGCt > 1:287003/1‑149 (MQ=37) gCC‑GCTTTGCGGCCCTTCGCCGGCAAGCACGCACCAAAAAAAGATATGCGTCCACTGTAGGAGAGGGCTTG‑CAAGCGAAGGGCTGCAAGGCAGCCCCAAATTGACGACTACGCGGATAAGCGATACGGCAGGGTCATGTTAAGCta > 8:246503/1‑145 (MQ=255) ggCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag < 8:58910/149‑1 (MQ=255) ttCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGcc > 5:186595/1‑149 (MQ=255) aaGCCCGCTCCTACAAAGGTTATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 2:30311/149‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg < 8:89894/149‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 6:342239/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 4:169919/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 8:244405/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 8:61859/1‑149 (MQ=255) atGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc > 3:129613/1‑149 (MQ=255) gCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGCTATGCAAATCATCGt > 3:249771/1‑149 (MQ=255) gCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 3:4234/1‑149 (MQ=255) cGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt < 8:176368/149‑1 (MQ=255) tCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 5:342239/149‑1 (MQ=255) ccccTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc > 8:89272/1‑149 (MQ=255) cccTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc > 4:293658/1‑148 (MQ=255) cccTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc < 7:89272/148‑1 (MQ=255) cccTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCACAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc > 4:293661/1‑148 (MQ=255) ccTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa < 4:4234/149‑1 (MQ=255) cTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc > 2:350512/1‑145 (MQ=255) cTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 1:350512/145‑1 (MQ=255) | TTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACG‑AAAATAGCAGTAAACGCTGGGGCC‑GCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGA > NC_002947/1932501‑1932768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |