Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 3,845,705 +G intergenic (‑72/+26) PP_3394 ← / ← PP_3395 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,845,7031.G93.8% 151.8 / 3.9 48intergenic (‑70/+28)PP_3394/PP_33953‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (28/17);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑C‑G‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGC  >  NC_002947/3845558‑3845844
                                                                                                                                                     |                                                                                                                                              
ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGCTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑C‑g‑gg                                                                                                                                            <  6:365653/149‑2 (MQ=255)
 tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑C‑g‑ggc                                                                                                                                           >  1:149072/1‑147 (MQ=255)
        ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCa                                                                                                                                     <  5:13053/149‑1 (MQ=255)
          cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATc                                                                                                                                   <  8:114070/149‑1 (MQ=255)
           aTGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCg                                                                                                                                  >  1:226445/1‑149 (MQ=255)
              aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCa                                                                                                                               <  2:78345/149‑1 (MQ=255)
                     gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGATGTATGACGGGCAGAAAAGAA‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGa                                                                                                                        >  3:223625/1‑149 (MQ=255)
                      cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGAt                                                                                                                       >  2:254488/1‑149 (MQ=255)
                      cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGAt                                                                                                                       >  5:320421/1‑149 (MQ=255)
                      cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGAt                                                                                                                       <  3:130063/149‑1 (MQ=255)
                                aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGccc                                                                                                             <  1:254488/149‑1 (MQ=255)
                                   ccATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCcgcg                                                                                                          >  1:159107/1‑149 (MQ=255)
                                    cATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCgcgc                                                                                                         >  6:189390/1‑149 (MQ=255)
                                             ggCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGA‑CCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGc                                                                                               <  4:77699/149‑1 (MQ=255)
                                               ccGACTTCATTGATTGTTATTGTTGTCATCGCGGGCTCTCTGGCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGcc                                                                                              <  7:235392/149‑1 (MQ=255)
                                                  aCTTCATTGATTGTTATTGTTGTCATCGGGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCATAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGTATCAGCCCGCGCCCTGCCAGGCCGGt                                                                                           <  6:147641/149‑1 (MQ=255)
                                                        ttgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTGGCTGACGATGGATCa                                                                                                                 >  6:206407/1‑121 (MQ=38)
                                                        ttgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTGGCTGACGATGGATCa                                                                                                                 <  5:206407/121‑1 (MQ=38)
                                                          gattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcag                                                                                   >  3:120952/1‑149 (MQ=255)
                                                           attgtaatggtgGTCCTCGCGGTCGCGCTGCCTTACGGGTGCCCCCCCACTAAGAGCTGGATCAGGGGCACAAACCCG‑GTGTTGCGG‑G‑G‑GCTTACCCCCCGCCCCCCACCATGGATCTCCCCGCGCCCTGCCAGGCCGGTGCACgcag                                                                                   <  4:120952/148‑1 (MQ=255)
                                                                    gttgtCATCGCGGTCTCTCTGTCTGAAGCGATGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCCGTGCACGCAGCAACAgctcg                                                                         >  7:40700/1‑145 (MQ=255)
                                                                    gttgtCATCGCGGGCTCGCTGTCTGAACCGTTGCGCCAGACTAACAGCTGCATGACGGGCACAAACGAG‑GGGTTTC‑G‑CGG‑GCGGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtg                                                                          <  6:63956/148‑1 (MQ=255)
                                                                       gtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑C‑GTGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc                                                                       >  3:65597/1‑148 (MQ=255)
                                                                           tCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAg                                                                    >  3:158204/1‑147 (MQ=255)
                                                                           tCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGAAGCAACAGGTGGTCAAg                                                                    >  4:299708/1‑147 (MQ=255)
                                                                           tCGCGGTCTCTCCGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAg                                                                                                 >  3:212362/1‑118 (MQ=255)
                                                                            cgcgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGACAAGCAg                                                                 >  6:253533/1‑149 (MQ=255)
                                                                                gTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAg                                                                 >  3:142618/1‑145 (MQ=255)
                                                                                     tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg                                                                            >  5:229025/1‑129 (MQ=255)
                                                                                      ctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGGTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc                                                        >  1:268434/1‑149 (MQ=255)
                                                                                           tgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGCTCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAg                                                  >  6:263855/1‑149 (MQ=255)
                                                                                           tgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAg                                                  >  3:305656/1‑149 (MQ=255)
                                                                                            gAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGCC‑GTGTTTC‑G‑CGG‑GCTCATCCATCGTCAGCCACGATGGATCCGCCCGCGCCCTGCCAGGCCGGTGCACGAAGCCACCGGGGGTCCACCAGGCCCCGCGCCGACAGc                                                 >  8:63558/1‑149 (MQ=255)
                                                                                            gAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc                                                 >  4:149973/1‑149 (MQ=255)
                                                                                             aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGACAAGCAGGCGCCGAGCCGACAGc                                                 <  5:253533/148‑1 (MQ=255)
                                                                                               gCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc                                                 >  7:186926/1‑146 (MQ=255)
                                                                                                cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCgaga                                             <  8:186926/149‑1 (MQ=255)
                                                                                                      gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt                                       >  6:101379/1‑149 (MQ=255)
                                                                                                             tAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTc                                >  5:138139/1‑149 (MQ=255)
                                                                                                              aaGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCg                                   >  5:327726/1‑145 (MQ=255)
                                                                                                              aaGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCg                                   <  6:327726/145‑1 (MQ=255)
                                                                                                               agagCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgc                              >  8:1386/1‑149 (MQ=255)
                                                                                                                    tGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg                         >  3:16450/1‑149 (MQ=255)
                                                                                                                      gATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg                         <  6:138139/147‑1 (MQ=255)
                                                                                                                      gATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg                         >  4:257145/1‑147 (MQ=255)
                                                                                                                          aCGGGCTGAAAAGAC‑GTGTTTC‑G‑CGG‑GCTGATCCATCGTCAGCAACGTTGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGAt                    <  2:226445/148‑1 (MQ=255)
                                                                                                                           ggggCATAAAAGCC‑GTGTTCC‑G‑CGG‑GCTCAGCCACCGCCCGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGa                  <  5:189390/148‑1 (MQ=255)
                                                                                                                               cataaaaGAC‑GTGTTTC‑GAG‑G‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGaa              <  4:223625/146‑1 (MQ=255)
                                                                                                                                   aaaTAC‑GTGTTTC‑G‑CGG‑GCTGATCCTTCGTCAGCAACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTc           <  4:65597/148‑1 (MQ=255)
                                                                                                                                            gtTTC‑G‑CGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgcgc  >  3:61666/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
CTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAC‑GTGTTTC‑G‑C‑G‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGC  >  NC_002947/3845558‑3845844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑