Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	307,866	+C	intergenic (+881/‑988)	hslO → / → PP_0254	molecular chaperone HslO/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	307,865	1	.	C	100.0%	201.0 / NA	59	intergenic (+880/‑989)	hslO/PP_0254	molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (29/30);  total (29/30)

AAAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307717‑308008                                                                                                                                                        |                                                                                                                                                aaaGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAg                                                                                                                                                  >  3:285738/1‑149 (MQ=255)   aGCCATGTTCTCGGGGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGTC‑AGCCGAAAggg                                                                                                                                                <  7:133344/149‑3 (MQ=255)     ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAG‑ccgt                                                                                                                                             >  6:136154/1‑146 (MQ=255)     ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAG‑ccgt                                                                                                                                             <  4:118989/149‑4 (MQ=255)        tGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGACAGCCGTTAc                                                                                                                                           >  4:227659/1‑149 (MQ=255)          ttCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTAcc                                                                                                                                          <  5:258467/148‑1 (MQ=255)          ttCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAG‑ccgtt                                                                                                                                            <  7:287823/145‑5 (MQ=255)          ttCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAG‑ccgtt                                                                                                                                            >  8:287823/1‑141 (MQ=255)            ctcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGa                                                                                                                                       >  5:135546/1‑149 (MQ=255)                 tGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACCTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCgg                                                                                                                                   <  1:258808/149‑1 (MQ=255)                         ttCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTgacgac                                                                                                                            >  1:260281/1‑147 (MQ=255)                                  ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGtt                                                                                                                  <  6:238291/148‑1 (MQ=255)                                  ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTg                                                                                                                 <  3:151184/149‑1 (MQ=255)                                    ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTggg                                                                                                               <  8:229229/149‑1 (MQ=255)                                      ctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGCGCGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                              <  1:253826/149‑1 (MQ=255)                                         ccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGaa                                                                                                          <  6:135546/149‑1 (MQ=255)                                                aCTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGTCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                    <  7:28709/148‑1 (MQ=255)                                                aCTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCGAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTg                                                                                                   >  8:117511/1‑149 (MQ=255)                                                 cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt                                                                                                  <  5:136154/149‑1 (MQ=255)                                                    ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt                                                                                               >  5:337303/1‑149 (MQ=255)                                                     cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc                                                                                              <  7:243908/149‑1 (MQ=255)                                                     cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCGTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc                                                                                              <  8:170489/149‑1 (MQ=255)                                                        tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                            <  2:281242/148‑1 (MQ=255)                                                        tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                            <  6:337303/148‑1 (MQ=255)                                                               cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa                                                                                     <  1:17272/148‑1 (MQ=255)                                                                        cATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTg                                                                                                                                        >  5:260824/1‑88 (MQ=255)                                                                        cATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTg                                                                                                                                        <  6:260824/88‑1 (MQ=255)                                                                             gCGAATAAGGCGATGTGACCTTCTTCTTCGGCATGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                       >  8:4674/1‑148 (MQ=255)                                                                             gCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                       >  7:215718/1‑148 (MQ=255)                                                                             gCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                       >  3:2481/1‑148 (MQ=255)                                                                                      gCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGTCGAGCACGGTTTGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCAt                                                              <  4:285738/148‑1 (MQ=255)                                                                                         aTGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                          <  8:215718/149‑1 (MQ=255)                                                                                           gtgACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCt                                                        >  2:267316/1‑149 (MQ=255)                                                                                              aCCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTc                                                     <  2:109367/149‑1 (MQ=255)                                                                                              aCCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGATACGCCAAGTGCATCGACCTGTc                                                     >  5:308209/1‑149 (MQ=255)                                                                                               ccTTGTTCTTCGGCCTGTCCGGCA‑CTGGCGAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                    <  7:117511/149‑1 (MQ=255)                                                                                                   gTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgag                                                 <  4:2481/148‑1 (MQ=255)                                                                                                     tcttcGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgag                                                 >  8:195862/1‑146 (MQ=255)                                                                                                       ttcGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATc                                                             <  1:309510/132‑1 (MQ=255)                                                                                                       ttcGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATc                                                             >  2:309510/1‑132 (MQ=255)                                                                                                       ttcGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                            >  8:66364/1‑149 (MQ=255)                                                                                                       ttcGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                            >  4:220545/1‑149 (MQ=255)                                                                                                        tcGTCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACTACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                            <  6:308209/148‑1 (MQ=255)                                                                                                          ggCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGa                                         <  4:192295/149‑1 (MQ=255)                                                                                                           gCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACGTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTCTCTTCAACATGGAAGGCCGCTGCTACGCCAAGTGCATCGACCAGTCCGAGAAGAACGAg                                        >  3:217565/1‑149 (MQ=255)                                                                                                           gCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAg                                        >  5:274173/1‑149 (MQ=255)                                                                                                           gCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAg                                        >  3:44242/1‑149 (MQ=255)                                                                                                               gTCCGGCA‑CTGGCAAGACTACCCTTTCCGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGCTTGGGCCGAAGGCGTTCTCTTCAACAAGGAAGGCGGCTCCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCgg                                    >  7:163868/1‑149 (MQ=255)                                                                                                                    gCA‑CTGGCAAGACTACGCTTTCGGC‑TGACGAAAGCCGTTACGTGATCGGTGAGGACGAGCACGGTTGGGGCGAAGGCGTTGTAGTCAACATGGAAGGCGGCTGCTACGGCGAGTGCATCGAGCTGTCCGAGAAGCACGCGCGGGTCATc                               >  4:182966/1‑149 (MQ=255)                                                                                                                          ggCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGa                           >  7:31774/1‑148 (MQ=255)                                                                                                                           gCAAGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGa                           <  6:12079/147‑1 (MQ=255)                                                                                                                             aaGACTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGc                       >  7:32647/1‑149 (MQ=255)                                                                                                                                 cTACCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                    >  5:22279/1‑148 (MQ=255)                                                                                                                                   aCCCTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                   >  8:144330/1‑147 (MQ=255)                                                                                                                                    cccTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg                <  3:220545/149‑1 (MQ=255)                                                                                                                                    cccTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg                <  3:227659/149‑1 (MQ=255)                                                                                                                                     ccTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag                                              >  6:214784/1‑118 (MQ=255)                                                                                                                                     ccTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag                                              <  5:214784/118‑1 (MQ=255)                                                                                                                                     ccTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg                >  4:121643/1‑148 (MQ=255)                                                                                                                                      cTTTCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCTAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCa              <  7:144330/149‑1 (MQ=255)                                                                                                                                       tttCGGC‑TGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCAc             >  5:126267/1‑149 (MQ=255)                                                                                                                                               tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACgg           >  7:81575/1‑144 (MQ=255)                                                                                                                                               tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACgg           <  8:81575/144‑1 (MQ=255)                                                                                                                                                   gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACt  >  4:294914/1‑149 (MQ=255)                                                                                                                                                   gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACt  <  8:32647/149‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                AAAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCA‑CTGGCAAGACTACCCTTTCGGC‑TGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307717‑308008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑