| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 88.2% | 72.6 / 1.7 | 35 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (14/16); minor base A (4/0); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 100.0% | 97.9 / NA | 35 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (18/17); total (18/17) | |||||||||||
GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGG‑C‑ACG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAT > NC_002947/1499361‑1499640 || gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑Acg‑cc > 3:241702/1‑149 (MQ=37) gAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGGCCGCGGAACAGCCCCCGCCGTGCAAGGCACCCGCGTTGCCCGTTTTAGCGGG‑C‑Ccg‑cccgc > 5:208249/1‑146 (MQ=255) gCCTTAACTACGCGTGGGCACCCCAGCTGCACTTATGGGGCTAAAGGGGGGGTGGGGGGGTGCCCGGTAAACTGGGGCCGGGGTCCAGGGCACCGTCTTTGCGGTTGTT‑GGGGG‑CAACGCCC‑GCTCCCACAGACTGATTGCCATTAAg < 6:208249/148‑1 (MQ=255) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGc < 4:241702/149‑1 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCgg < 5:145936/149‑1 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCgg > 5:357342/1‑149 (MQ=25) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACAGGCCCCGCGGTGCATGGCAACCGCATTGCCGGTCTTCGCGGG‑C‑ACGCCC‑CCAACCACAGACAGAATGCCATTAAGCGGGc > 4:251828/1‑149 (MQ=255) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGG‑C‑ACGCCC‑GCTCCCACAGACGGATTGGCATTAAGCGGGCCGATc > 1:223791/1‑149 (MQ=21) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCAGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc > 6:163692/1‑149 (MQ=25) acaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACAGGCGCCGCCGGGCATGGCACCGGCGTTGCCCGTGTTCGCGGGCC‑CCC‑CC‑CCGCCCACACACAGATTGGCCTTAAGCCGGCCAATCGCTAAAGTGACgg > 5:63830/1‑149 (MQ=255) gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAGGCGCCGCGGGTCATGGCACCGGCTTTGCCGGTTTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc < 7:202216/149‑1 (MQ=25) cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt > 3:226147/1‑149 (MQ=25) ccAAACGGGGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc < 5:152227/147‑1 (MQ=21) gcaggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 5:163692/146‑1 (MQ=25) gCGGGCGTGCCCGCTAAACCGGCGCCGCGGTGCAGGGCACCGGCTTGGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:139148/149‑1 (MQ=25) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 6:121479/1‑149 (MQ=37) cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCTCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 6:63830/149‑1 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 4:47780/1‑148 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:355821/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 6:227085/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 7:103934/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 6:357342/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa > 6:199874/1‑149 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg > 8:6292/1‑118 (MQ=21) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg < 7:6292/118‑1 (MQ=21) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa > 4:20271/1‑140 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 3:20271/140‑1 (MQ=25) cgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 7:147788/133‑1 (MQ=25) cgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt > 8:147788/1‑133 (MQ=25) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 5:199874/149‑1 (MQ=37) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 3:251828/149‑1 (MQ=37) ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 3:246295/149‑1 (MQ=37) gcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 8:15477/1‑146 (MQ=37) ccGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtctt < 5:121479/149‑1 (MQ=255) ccGGTGTTCGCGGG‑C‑ACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtctt < 4:226147/149‑1 (MQ=255) tgttcgcGGG‑A‑ACG‑CCAGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 4:60827/143‑1 (MQ=255) || GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGG‑C‑ACG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAT > NC_002947/1499361‑1499640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |