| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,586,030 | 2 bp→TC | intergenic (+113/+101) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,030 | 0 | C | T | 95.2% | 56.3 / ‑4.3 | 21 | intergenic (+113/+102) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (5/15); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
| * | NC_002947 | 4,586,031 | 0 | T | C | 95.2% | 59.5 / ‑6.1 | 21 | intergenic (+114/+101) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (5/15); minor base G (1/0); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAC > NC_002947/4585887‑4586103 || gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGC‑gg < 5:235874/149‑1 (MQ=255)gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGC‑gg < 8:113486/149‑1 (MQ=255)gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGC‑gg < 2:306074/149‑1 (MQ=255) ggTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCa < 2:356963/149‑1 (MQ=255) gTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAg < 7:322839/149‑1 (MQ=255) ttAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGA‑ccgtg > 3:5336/1‑145 (MQ=39) aaGGGTAGACCACGGCACCGGCGTGAACAGCTGATATGCGCTGCTCCAATGAAAGGTTGAGGATGACCGGGTGCATGGCAAGGTGAAGATCTTGCATTGATCTGGCCGGCCTGTTCGAG‑GGTAAACCCGCTCCTACCAGGGA‑ccgtgca > 4:96107/1‑143 (MQ=255) tAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCt < 6:231310/149‑1 (MQ=39) gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCtt < 4:342727/148‑1 (MQ=39) ggCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg < 3:35803/149‑1 (MQ=39) ggCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg < 4:5336/149‑1 (MQ=39) gCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcgg < 1:230970/149‑1 (MQ=39) gTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCA‑CTTCGCGGGTAAACCCGCACCTAACAGGGACCGTGGCGGCCGTGAATGAGGCGCTGgcc > 5:76011/1‑149 (MQ=255) tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGATCCTACCAGGGACCGTGCGGGCCTGGATTGCGGCGCTGCCCGTGGTAGGAGCggg > 8:179889/1‑149 (MQ=38) cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTCCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACGAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCGCTGGTAGGAGCgg < 3:96107/146‑1 (MQ=37) cgcTGCTCCATTGAAAGGGTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGc < 5:30457/144‑1 (MQ=38) ctCCAATGCAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAc < 7:179889/147‑1 (MQ=37) gACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg > 5:283400/1‑114 (MQ=37) gACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg < 6:283400/114‑1 (MQ=37) ggccggccT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGa < 7:69509/77‑1 (MQ=18) ggccggccT‑CTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGa > 8:69509/1‑77 (MQ=18) || GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCT‑CTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAC > NC_002947/4585887‑4586103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |