| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 97.7% | 127.3 / ‑5.0 | 44 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (22/21); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 95.5% | 127.9 / NA | 44 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (21/21); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑CACGGCTTTGCCGGTGTT‑TCGGG‑C‑ACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > NC_002947/1499344‑1499611 || ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGTCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCg < 1:38778/149‑1 (MQ=255)ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCg < 5:293243/149‑1 (MQ=255) gCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGGGCGGGCGTGCCCGCGAAAAAAGCCCCCCCGTGCATTGC‑AACCGCATTGCCgg > 6:152227/1‑149 (MQ=255) cAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGcc < 1:284547/143‑1 (MQ=255) cAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGcc > 2:284547/1‑143 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGACGCATTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGttcgcgg < 6:201224/148‑6 (MQ=37) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCCAAACCGGCCCCGCGGTGCCTGGCACCCGGGTTT‑CCGGTGttcgccgg > 5:328234/1‑143 (MQ=255) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑Acg‑cc > 3:241702/1‑149 (MQ=37) tGAAGCCCGTGATGCGGCCATTCAGAGCCTTAACGAAGCCTTTGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGgt < 4:318428/132‑1 (MQ=255) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACAACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGCGCGAGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGCCTTTGCCGgt > 3:318428/1‑132 (MQ=255) gAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGGCCGCGGAACAGCCCCCGCCGTGCAAGGC‑ACCCGCGTTGCCCGTTTTAGCGGG‑C‑Ccg‑cccgc > 5:208249/1‑146 (MQ=255) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGgt > 6:177835/1‑128 (MQ=255) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGgt < 5:177835/128‑1 (MQ=255) gCCTTAACTACGCGTGGGCACCCCAGCTGCACTTATGGGGCTAAAGGGGGGGTGGGGGGGTGCCCGGTAAACTGGGGCCGGGGTCCAGGGC‑ACCGTCTTTGCGGTTGTT‑GGGGG‑CAACGCCCGCTCCCACAGACTGATTGCCATTAAg < 6:208249/148‑1 (MQ=255) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGc < 4:241702/149‑1 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 5:145936/149‑1 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTAGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg > 5:357342/1‑149 (MQ=25) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACAGGCCCCGCGGTGCATGGC‑AACCGCATTGCCGGTCTTCGCGGG‑C‑ACGCCCCCAACCACAGACAGAATGCCATTAAGCGGGc > 4:251828/1‑149 (MQ=255) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTAGCGGG‑C‑ACGCCCGCTCCCACAGACGGATTGGCATTAAGCGGGCCGATc > 1:223791/1‑149 (MQ=21) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCAGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc > 6:163692/1‑149 (MQ=25) acaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACAGGCGCCGCCGGGCATGGC‑ACCGGCGTTGCCCGTGTTCGCGGGCC‑CCC‑CCCCGCCCACACACAGATTGGCCTTAAGCCGGCCAATCGCTAAAGTGACgg > 5:63830/1‑149 (MQ=255) gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAGGCGCCGCGGGTCATGGC‑ACCGGCTTTGCCGGTTTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc < 7:202216/149‑1 (MQ=25) cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTAGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt > 3:226147/1‑149 (MQ=25) ccAAACGGGGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc < 5:152227/147‑1 (MQ=21) gcaggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 5:163692/146‑1 (MQ=25) gCGGGCGTGCCCGCTAAACCGGCGCCGCGGTGCAGGGC‑ACCGGCTTGGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:139148/149‑1 (MQ=25) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 6:121479/1‑149 (MQ=37) cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCTCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 6:63830/149‑1 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:355821/1‑148 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 4:47780/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 7:103934/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 6:227085/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 6:357342/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa > 6:199874/1‑149 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg < 7:6292/118‑1 (MQ=21) cgcgAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg > 8:6292/1‑118 (MQ=21) cgcgAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 3:20271/140‑1 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa > 4:20271/1‑140 (MQ=25) cgAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 7:147788/133‑1 (MQ=25) cgAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt > 8:147788/1‑133 (MQ=25) aaaCAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 5:199874/149‑1 (MQ=37) aaaCAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 3:251828/149‑1 (MQ=37) ggCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 3:246295/149‑1 (MQ=37) gcgGTGCATGGC‑ACCGGCTTTGCCGGTGTTCGCGGG‑C‑ACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 8:15477/1‑146 (MQ=37) || GGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑CACGGCTTTGCCGGTGTT‑TCGGG‑C‑ACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > NC_002947/1499344‑1499611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |