Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,948,661	Δ1 bp	5.5%	coding (187/435 nt)	fliO ←	flagellar protein FliO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,948,661	0	T	.	5.5%	234.1 / 6.8	73	coding (187/435 nt)	fliO	flagellar protein FliO
Reads supporting (aligned to +/- strand):  ref base T (40/29);  new base . (2/2);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

CCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCAGGCAGGCGTGG  >  NC_002947/4948514‑4948809                                                                                                                                                     |                                                                                                                                                       ccAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGctc                                                                                                                                                       <  4:124350/149‑1 (MQ=255)  cAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCATGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGctct                                                                                                                                                      >  7:244039/1‑149 (MQ=255)  cAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGctct                                                                                                                                                      <  5:226927/149‑1 (MQ=255)    gTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCTGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTg                                                                                                                                                     >  6:16151/1‑148 (MQ=255)         tGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑gcg                                                                                                                                              >  5:128511/1‑149 (MQ=255)          gCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑gcgc                                                                                                                                             <  4:165402/149‑1 (MQ=255)          gCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑gcgc                                                                                                                                             >  1:347870/1‑149 (MQ=255)           cAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑gcgc                                                                                                                                             <  2:347870/148‑1 (MQ=255)                 cTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTg                                                                                                                                                     <  3:343984/135‑1 (MQ=255)                 cTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTg                                                                                                                                                     >  4:343984/1‑135 (MQ=255)                    gATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑Gcagcag                                                                                                                                    <  7:210191/147‑1 (MQ=255)                    gATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgcc                                                                                                                                  >  4:93600/1‑149 (MQ=255)                     ctGGTGCCGGGGGTATGGCCGATCAGGCTCTGCTCCTTGCCCCCCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCGCTGCGGCGC‑CTGCAGAGCACC‑gcagtgaagg                                                                                                                                 <  5:211844/148‑7 (MQ=255)                       ggTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccagg                                                                                                                               >  2:261645/1‑149 (MQ=255)                        gTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccaggc                                                                                                                              <  6:10125/149‑1 (MQ=255)                          gCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGTGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccaggc                                                                                                                              >  1:289871/1‑148 (MQ=255)                             gggggTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCagaa                                                                                                                         <  6:273787/149‑1 (MQ=255)                              ggggTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCaga                                                                                                                          >  2:211289/1‑147 (MQ=255)                              ggggTATGGCCGATCAGGATCTGATCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCTGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCaga                                                                                                                          >  8:92808/1‑147 (MQ=255)                               gggTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCagaaag                                                                                                                       <  3:313725/149‑1 (MQ=255)                                  tATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAaagaag                                                                                                                    <  6:10268/149‑1 (MQ=255)                                       ccGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGAGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGAC‑GCAGCAGCCAGGCCAGAAAGAAGATCAg                                                                                                               >  1:268836/1‑149 (MQ=255)                                        cGATCAGGATCTGCTCCTTGCCCAACTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAgg                                                                                                              <  8:244039/149‑1 (MQ=255)                                          aTCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccaggcca                                                                                                                            <  5:106242/133‑1 (MQ=255)                                          aTCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccaggcca                                                                                                                            >  6:106242/1‑133 (MQ=255)                                              ggATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCccacc                                                                                                        >  5:22943/1‑149 (MQ=255)                                                        cTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagc                                                                                               <  1:351133/148‑1 (MQ=255)                                                         ttGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgcca                                                                                                                                 >  3:22827/1‑113 (MQ=255)                                                         ttGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgcca                                                                                                                                 <  4:22827/113‑1 (MQ=255)                                                            cccACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGcc                                                                                          <  5:49921/149‑1 (MQ=255)                                                             ccACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGcc                                                                                          >  7:29723/1‑148 (MQ=255)                                                             ccACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGcc                                                                                          >  6:140488/1‑148 (MQ=255)                                                              cACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGccc                                                                                         >  4:257364/1‑148 (MQ=255)                                                                ccTGCACCAGCAGCAGGCG‑GTCACGCGGTCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcag                                                                                                   <  4:126623/136‑1 (MQ=255)                                                                ccTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAg                                                                                      >  3:259287/1‑149 (MQ=255)                                                                ccTGCACCAGCAGCAGGCG‑GTCACGCGGACCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcag                                                                                                   >  3:126623/1‑136 (MQ=255)                                                                 cTGCACCAGCAGCAGGCG‑GTCACGCGGGCCTATGGCACGGCTGCAGACAATCTCGATCACCTGCCCACCCTTCACTGCTGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGTCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGa                                                                                     <  2:268836/149‑1 (MQ=255)                                                                  tGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGAc                                                                                    >  5:368838/1‑149 (MQ=255)                                                                   gCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGAc                                                                                    >  3:80356/1‑148 (MQ=255)                                                                    cACCAGCAGCAGGCG‑GTCACGCGGGCTGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCa                                                                                  <  5:16151/149‑1 (MQ=255)                                                                     aCCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCAt                                                                                 >  8:215018/1‑149 (MQ=255)                                                                       cagcagcagGCG‑GTCACGCGGGCCGATGGCACGGCTTCCGACAATCTCGATCACCTGCCCACCATTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCCGCAGCAGGCCCAAGACCATCt                                                                               >  5:19694/1‑149 (MQ=255)                                                                        agcagcagGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTg                                                                              >  2:295706/1‑149 (MQ=255)                                                                              agGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAg                                                                        >  5:342616/1‑149 (MQ=255)                                                                               ggCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCccac                                                                                                         <  8:87081/115‑1 (MQ=255)                                                                                     tCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGccc                                                                  >  6:77108/1‑149 (MQ=255)                                                                                         gcgGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagc                                                                                               >  3:298646/1‑116 (MQ=255)                                                                                         gcgGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagc                                                                                               <  4:298646/116‑1 (MQ=255)                                                                                                     cACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGt                                                  <  4:259287/149‑1 (MQ=255)                                                                                                        ggCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGa                                               <  6:368838/149‑1 (MQ=255)                                                                                                          cTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCACGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAgc                                             <  2:289871/149‑1 (MQ=255)                                                                                                                  aaTCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCgggg                                     >  6:367389/1‑149 (MQ=255)                                                                                                                   aTCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGt                                    <  1:295706/149‑1 (MQ=255)                                                                                                                      tcGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgc                                 >  3:363912/1‑149 (MQ=255)                                                                                                                      tcGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgc                                 >  5:275605/1‑149 (MQ=255)                                                                                                                          tCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGTCCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgctgct                             <  4:246497/149‑1 (MQ=255)                                                                                                                           cACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGACAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGctgctg                            <  8:121156/149‑1 (MQ=255)                                                                                                                             ccTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGc                          >  2:315992/1‑149 (MQ=255)                                                                                                                               tGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCTGCAGCAGCCAGGCTAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCg                         >  1:239105/1‑149 (MQ=255)                                                                                                                                 cccACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTgg                      >  2:372963/1‑149 (MQ=255)                                                                                                                                   cACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGcc                    >  6:186034/1‑149 (MQ=255)                                                                                                                                   cACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGcc                    >  1:39997/1‑149 (MQ=255)                                                                                                                                   cACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGcc                    >  4:128756/1‑149 (MQ=255)                                                                                                                                    aCCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCg                   <  3:128756/149‑1 (MQ=255)                                                                                                                                       cTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAg                <  1:52184/149‑1 (MQ=255)                                                                                                                                          cACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCca             >  7:266119/1‑149 (MQ=255)                                                                                                                                           gcTGCGGCGC‑CTGCAGAGCACC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTg                       <  6:52108/137‑1 (MQ=255)                                                                                                                                           gcTGCGGCGC‑CTGCAGAGCACC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTg                       >  5:52108/2‑138 (MQ=255)                                                                                                                                           aCTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcag            >  6:187968/1‑149 (MQ=255)                                                                                                                                           aCTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcag            <  5:339880/149‑1 (MQ=255)                                                                                                                                           aCTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcag            <  6:22943/149‑1 (MQ=255)                                                                                                                                            cTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcagg           >  4:202913/1‑149 (MQ=255)                                                                                                                                            cTGCGGCGC‑CTGCAGAGCACC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcagg           >  6:345352/1‑149 (MQ=255)                                                                                                                                             tGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcaggc          >  3:225482/1‑149 (MQ=255)                                                                                                                                                 gTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCaggcaggc      <  6:342616/149‑1 (MQ=255)                                                                                                                                                  tGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCAGGCAGGCg     >  4:352097/1‑149 (MQ=255)                                                                                                                                                    ctctGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCAGGCAGGCGt    <  4:200957/148‑1 (MQ=255)                                                                                                                                                     tctGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCAGGCAGGCGtgg  >  3:66743/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                       CCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCAGGCAGGCGTGG  >  NC_002947/4948514‑4948809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑