| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,655,641 | Δ1 bp | 9.3% | intergenic (‑96/+56) | epd ← / ← tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,655,641 | 0 | T | . | 9.3% | 136.0 / 11.2 | 54 | intergenic (‑96/+56) | epd/tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Reads supporting (aligned to +/- strand): ref base T (23/23); new base . (3/2); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01 | |||||||||||
ATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACTCACC > NC_002947/5655499‑5655789 | aTACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGTGCATTCGGGGCTCACGAGACAGTTCAATTGTCTTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTgg < 6:228269/149‑1 (MQ=255)aTACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTgg < 8:118730/149‑1 (MQ=255) ggCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGc > 4:356549/1‑149 (MQ=255) cATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCggg > 6:381733/1‑149 (MQ=255) atatCCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGc < 6:32977/149‑1 (MQ=37) tGCAACTTTGTCGGGACGGTGGGGCATTCGGGGCGCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTGGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAt < 7:53306/149‑1 (MQ=255) aaCTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑Ctgtg > 7:55926/1‑125 (MQ=37) aaCTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑Ctgtg < 8:55926/125‑1 (MQ=37) aaCTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGGCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGaa < 4:257547/149‑1 (MQ=255) cTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGTCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAgg < 2:225226/149‑1 (MQ=255) gggggCATTCGGGGCTCACGATACAGTTAAATTTTCGTCAGCCTGCGGAATTTGCGGTTTGGCATTCTC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATTCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCa < 6:371250/148‑1 (MQ=38) ggCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGAt > 5:95183/1‑149 (MQ=255) aTTCGGGGCTCACGAGACATTACAATTGACGTCACCCTGCGTAATTGGCGGTGGGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCACCCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCtt < 7:125728/149‑1 (MQ=37) aTTCGGGGCTCACGAGACAGTTCAATTGTCGTCACCCGGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGTAGCGGGCTTGCCCGCGAATGAAGGCGACAccgcc < 2:210972/139‑1 (MQ=255) aTTCGGGGCTCACGAGACAGTTCAATTGACGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGAAAccgcc > 1:210972/1‑139 (MQ=255) cGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTa < 3:340425/148‑1 (MQ=255) gggCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAAt > 8:252264/1‑149 (MQ=255) gCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCa < 3:356549/149‑1 (MQ=255) cACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAAt > 7:185035/1‑144 (MQ=255) cACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAAt < 8:185035/144‑1 (MQ=255) aCGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGt < 7:344151/147‑1 (MQ=255) aCGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTACCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGCCCCCGCCCGCAGATGGTTTAATCAGtgt > 8:190787/1‑147 (MQ=38) gagaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTc > 5:330412/1‑149 (MQ=255) aaTTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTGATGCAACCGCTGTGGGAGCGGGCATGCCCGCGAATGAAGGCGCCACCGCCCGCAGATGGTTAAATAAGTCTTGCAGCCGgtct > 4:337372/1‑149 (MQ=37) gtcAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTGCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCGTGCCCGCGAATGAAGGCGCCACCGCCCGCAGATCTTTAAATCAGGCGTGCAGCAGCTCTTGGGGAg > 5:5852/1‑149 (MQ=38) gtcAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCa < 6:95183/148‑1 (MQ=255) gCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTAcc > 7:71219/1‑149 (MQ=255) cTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTGTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTAcc > 5:78886/1‑147 (MQ=255) tGCGGAATTTGCGGTGTGGCATTCGCGGGGCCAGCCCCCGCCCACAG‑GGGGTGATGGAAACAGAGTGGGGGCGGGCTTGGCCGCCGATGAAGGAGCCCCCCCCCCCCGAGGATTAAATAAGTATTGAAGCAGAGCTTAGGCGCTACCgc > 2:324030/1‑148 (MQ=255) tGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg > 6:277452/1‑149 (MQ=255) ggAATCTGCGGGGTGGCATTCGC‑GGGCAAGCCCGCTCCCCCAG‑GGTTTCATCCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGAt < 4:205672/149‑1 (MQ=255) aTTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGtt < 3:33098/149‑1 (MQ=255) aTTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGtt < 5:277452/149‑1 (MQ=255) gCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTtctc < 1:5419/148‑1 (MQ=255) gtgtGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAgg < 8:244522/149‑1 (MQ=255) ccttcATTCGC‑GGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGt > 4:270258/5‑149 (MQ=21) tgGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGACCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATAAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGt > 6:385395/1‑148 (MQ=255) gc‑gGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCcag > 6:208123/1‑104 (MQ=17) gc‑gGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCcag < 5:208123/104‑1 (MQ=17) gggCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAAct > 3:288252/1‑149 (MQ=255) cAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCtt < 6:78886/149‑1 (MQ=255) ccGCTCCCCCAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTGTAAATCAGTCTTCCAGCACCTCTTCGGCAGTACCGACGATGTTCTCCAGGGTGTAGGCAAACTCTTCGaa > 1:325650/1‑148 (MQ=255) gCTCCCACTG‑GGTTTCATGAAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACaa < 1:324030/149‑1 (MQ=255) ccACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGcc > 8:191752/1‑149 (MQ=255) cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGcc > 2:272867/7‑148 (MQ=38) cacaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGcc > 3:237579/1‑148 (MQ=255) cacaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGcc > 7:149410/1‑148 (MQ=255) acaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCg > 7:178704/1‑148 (MQ=255) ctg‑ggTTTCTTGCAACCGCTGTGGGAGCGGGCTGGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGaa < 2:339222/147‑1 (MQ=255) caG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCtt < 5:200732/93‑1 (MQ=255) caG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCtt > 6:200732/1‑93 (MQ=255) ggTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATg > 6:284202/1‑107 (MQ=255) ggTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATg < 5:284202/107‑1 (MQ=255) tttCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCgg > 5:284743/1‑149 (MQ=255) aTGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGcc > 4:317372/1‑149 (MQ=255) aCCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCGGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACTc < 7:252264/149‑1 (MQ=255) tctGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACTCAcc > 2:101108/1‑149 (MQ=255) | ATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGC‑GGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACTCACC > NC_002947/5655499‑5655789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |