Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	979,599	Δ1 bp	6.1%	coding (1049/1215 nt)	iscS‑I →	cysteine desulfurase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	979,599	0	A	.	6.1%	209.9 / 7.2	66	coding (1049/1215 nt)	iscS‑I	cysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base . (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

CTGAACCTGAGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGTTGACATCTCCAAG  >  NC_002947/979451‑979744                                                                                                                                                      |                                                                                                                                                   ctgaacctgaGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGca                                                                                                                                                    <  3:235090/149‑1 (MQ=255)    accctgaTCTTTCACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGcac‑a                                                                                                                                                 <  1:129620/146‑1 (MQ=255)    aacctgaGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑Ag                                                                                                                                                <  8:348753/149‑1 (MQ=255)          aGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTGTGCGC‑C‑AGCTCgt                                                                                                                                           <  4:368315/148‑2 (MQ=255)           gCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTCCGTACTGCGTGCTCTGGGCCGCAACGACGAGGTGTGCGC‑C‑AGCTCgtcg                                                                                                                                         <  1:87794/149‑4 (MQ=255)              tCAGCTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGc                                                                                                                                      >  3:278834/1‑149 (MQ=255)              tCAACTACGTCGAAGGCGAATCGCTGCTTATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGc                                                                                                                                      >  5:236944/1‑149 (MQ=255)              tCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGc                                                                                                                                      >  6:379680/1‑149 (MQ=255)                 aCTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTtc                                                                                                                                   <  5:47310/149‑1 (MQ=255)                 aCTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTtc                                                                                                                                   <  3:334928/149‑1 (MQ=255)                    aCGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTtctc                                                                                                                                 <  4:188421/148‑1 (MQ=255)                        cGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCg                                                                                                                            >  4:62844/1‑149 (MQ=255)                          aaGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGc                                                                                                                          <  3:3569/149‑1 (MQ=255)                                        ctgATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCACAAGCTCGATCCGCTTCTCCTTCGGCCGTTTCaccacc                                                                                                              <  1:61595/148‑1 (MQ=255)                                             gTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCgaag                                                                                                          >  6:226357/1‑146 (MQ=255)                                                gCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGaagaagaag                                                                                                    >  7:70212/1‑149 (MQ=255)                                                gCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGaagaagaag                                                                                                    >  3:291009/1‑149 (MQ=255)                                                gCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGaagaagaag                                                                                                    >  8:184524/1‑149 (MQ=255)                                                 cTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGt                                                                                                   <  7:184524/149‑1 (MQ=255)                                                    aaaGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGa                                                                                                <  5:223197/149‑1 (MQ=255)                                                          aTCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTAcgc                                                                                          >  3:338924/1‑149 (MQ=255)                                                          aTCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTAcgc                                                                                          >  2:353938/1‑149 (MQ=255)                                                           tCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCTCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGttt                                                                                                                     <  7:55665/121‑1 (MQ=255)                                                           tCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGttt                                                                                                                     >  8:55665/1‑121 (MQ=255)                                                             gCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTAcgccgc                                                                                       <  6:236944/149‑1 (MQ=255)                                                                     gTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGaaa                                                                                <  5:339460/148‑1 (MQ=255)                                                                     gTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGaaa                                                                                <  5:283987/148‑1 (MQ=255)                                                                     gTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGa                                                                                  >  6:283987/1‑146 (MQ=255)                                                                     gTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAg                                                                               >  2:195097/1‑149 (MQ=255)                                                                          gTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGc                                                                          <  7:39148/149‑1 (MQ=255)                                                                          gTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGc                                                                          <  3:62844/149‑1 (MQ=255)                                                                           ttCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGc                                                                          >  1:15488/1‑148 (MQ=255)                                                                              ggCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGACTTTCTGCgcg                                                                       <  2:186508/148‑3 (MQ=255)                                                                              ggCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGATTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGatg                                                                      <  8:223207/149‑3 (MQ=255)                                                                                ccTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGcc                                                                    <  1:306259/149‑1 (MQ=255)                                                                                   gTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGt                                                                  >  7:4693/1‑148 (MQ=255)                                                                                    tACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGt                                                                  <  1:353938/147‑1 (MQ=255)                                                                                       cTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAAt                                                              >  1:11941/1‑148 (MQ=255)                                                                                          cgcggggCTCGAGCCGTCGTAAGTACTGCTTGCTGTGGGCCGCTACGACGAGCTG‑GCGCTC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCTCCACCGAAGAAGAAGTCGACTACGCCGCGCAGATAGTCTGCGAGGCCGTCAATaat                                                           <  1:74370/142‑2 (MQ=255)                                                                                          cgcgTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAAc                                                          >  2:40608/1‑149 (MQ=255)                                                                                              tCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCg                                                      >  8:116732/1‑149 (MQ=255)                                                                                                gCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCg                                                      >  3:345334/1‑147 (MQ=255)                                                                                                gCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCg                                                      <  4:345334/147‑1 (MQ=255)                                                                                                gCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGt                                                     <  4:278834/148‑1 (MQ=255)                                                                                                 cTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGa                                                   <  8:70212/149‑1 (MQ=255)                                                                                                 cTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGa                                                   <  8:4693/149‑1 (MQ=255)                                                                                                   cGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGc                                                 >  7:200522/1‑149 (MQ=255)                                                                                                    gAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTGTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCt                                                >  6:3920/1‑149 (MQ=255)                                                                                                       ccGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTc                                             <  5:226357/149‑1 (MQ=255)                                                                                                       ccGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTc                                             >  1:37285/1‑149 (MQ=255)                                                                                                         gTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTcgc                                           <  1:40608/149‑1 (MQ=255)                                                                                                                gTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCtgtg                                    >  5:108586/1‑149 (MQ=255)                                                                                                                 tACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTgggg                                   <  6:108586/149‑1 (MQ=255)                                                                                                                  aCTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCtgtg                                    <  5:217531/147‑1 (MQ=255)                                                                                                                  aCTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCtgtg                                    >  6:217531/1‑147 (MQ=255)                                                                                                                      cGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGata                               <  5:379680/148‑1 (MQ=255)                                                                                                                           tctGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGatat                              <  6:96902/144‑1 (MQ=255)                                                                                                                           tctGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGatat                              >  5:96902/1‑144 (MQ=255)                                                                                                                           tctGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTAc                          >  8:256793/1‑148 (MQ=255)                                                                                                                             tGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACa                         <  7:116732/147‑1 (MQ=255)                                                                                                                               ggCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGa                     >  3:357666/1‑149 (MQ=255)                                                                                                                                    cAACCACCAGCTG‑GCCCAC‑AGCTCGATCCGCTTCTCCTTCGGCCCTTTCACCACCCAAGAAGAACACGACTACGCCGCGCAGAAAGTCACCGAGGCCGGCAATAAACTGCGTGAGCTCTCGCAGCTGTGGCATATGTACAAACACGGCg                >  8:277315/1‑149 (MQ=255)                                                                                                                                     aaCGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGt               >  3:68677/1‑149 (MQ=255)                                                                                                                                       gatcgaGCTGTGCGC‑C‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCTAGAAGAAAGTCTGCGAGTAAGTCAATAAAATGCGTGAGATGTCGACGCTGTGGTATATGTACAAAGACGGAGtt              >  5:185734/4‑148 (MQ=255)                                                                                                                                       gatcgaGCTGTGCGC‑C‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGtt              >  7:155761/4‑148 (MQ=255)                                                                                                                                       cgacgaGCTG‑GCGCAC‑AGGTGGTTCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGTAGAAGTCGACTACGCCGGGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTGGCCGCTGTGGGATATGTACAAAGACGGCGTTg             <  8:200522/149‑1 (MQ=255)                                                                                                                                                   cgcAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGTTGACATCTCCAAg  <  2:11941/149‑1 (MQ=255)                                                                                                                                                    gcAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGTTGACATCTcc     >  3:364791/1‑145 (MQ=255)                                                                                                                                                      |                                                                                                                                                   CTGAACCTGAGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGTTGACATCTCCAAG  >  NC_002947/979451‑979744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑