Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,962,576	Δ1 bp	8.6%	coding (774/1152 nt)	PP_3492 →	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,962,576	0	A	.	8.6%	220.9 / 13.0	70	coding (774/1152 nt)	PP_3492	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (30/34);  new base . (3/3);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGC  >  NC_002947/3962433‑3962721                                                                                                                                                 |                                                                                                                                                   tGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCg                                                                                                                                                >  4:95751/1‑148 (MQ=255)  gcgcGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGC‑TTCGaat                                                                                                                                             >  3:258662/1‑148 (MQ=255)   cgcgGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑g                                                                                                                                            >  5:374622/1‑149 (MQ=255)      ggTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑gcgg                                                                                                                                         <  1:62559/149‑1 (MQ=255)          aCCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCt                                                                                                                                     >  6:353432/1‑149 (MQ=255)          aCCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCt                                                                                                                                     >  6:333890/1‑149 (MQ=255)          aCCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGC‑TTCGAATGCGgcgc                                                                                                                                      <  5:315173/148‑1 (MQ=255)            cTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGC‑TTCGAATGCGGCGCggg                                                                                                                                   <  4:258662/149‑1 (MQ=255)               cGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGt                                                                                                                                  <  2:90138/147‑1 (MQ=255)                  cAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCgggcgat                                                                                                                               >  5:90962/1‑143 (MQ=255)                  cAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCgggcgat                                                                                                                               <  6:90962/147‑5 (MQ=255)                     cTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTtctc                                                                                                                            <  6:151052/147‑1 (MQ=255)                      tGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTcgcg                                                                                                                         >  3:317265/1‑149 (MQ=255)                      tGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTcgcg                                                                                                                         >  6:156224/1‑149 (MQ=255)                        ccGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTg                                                                                                                       >  6:80400/1‑149 (MQ=255)                          gCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGAc                                                                                                                     <  1:238812/149‑1 (MQ=255)                            aTTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCg                                                                                                                   <  8:179702/149‑1 (MQ=255)                             ttCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCg                                                                                                                   >  8:323631/1‑148 (MQ=255)                                cTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATa                                                                                                               <  5:53234/149‑1 (MQ=255)                                  gccgccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCGGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACa                                                                                                             >  5:321215/1‑149 (MQ=255)                                     gccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGtt                                                                                                          >  4:116291/1‑149 (MQ=255)                                       cAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTg                                                                                                        <  5:202340/149‑1 (MQ=255)                                        aaTATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCTGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTgg                                                                                                       <  2:238841/149‑1 (MQ=255)                                            tCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCaa                                                                                                    <  2:335270/148‑1 (MQ=255)                                            tCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAg                                                                                                   <  6:215954/149‑1 (MQ=255)                                              cTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGc                                                                                                  <  6:321215/148‑1 (MQ=255)                                              cTCCGTGAGCGTGGCAAGGGCCTGGCCATTGCCCGGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCATGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGc                                                                                                  <  8:141661/148‑1 (MQ=255)                                               tCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTgg                                                                                                       >  6:335426/1‑142 (MQ=255)                                               tCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTgg                                                                                                       <  5:335426/142‑1 (MQ=255)                                                 ggTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGa                                                                                               <  1:78119/148‑1 (MQ=255)                                                   tGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTc                                                                                                                                 <  7:267145/112‑1 (MQ=255)                                                   tGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTc                                                                                                                                 >  8:267145/1‑112 (MQ=255)                                                   tGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCa                                                                                            <  1:360980/149‑1 (MQ=255)                                                            cAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                          >  7:62404/1‑142 (MQ=255)                                                            cAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                          <  8:62404/142‑1 (MQ=255)                                                             aaGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCa                                                                                  <  7:208330/149‑1 (MQ=255)                                                             aaGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCa                                                                                  <  1:168827/149‑1 (MQ=255)                                                                ggCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCagag                                                                               <  3:116291/149‑1 (MQ=255)                                                                 gCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCagag                                                                               >  2:257750/1‑148 (MQ=255)                                                                 gCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATAGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCagag                                                                               >  6:363326/1‑148 (MQ=255)                                                                     ggCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTg                                                                          >  7:273820/1‑149 (MQ=255)                                                                         aTTGCCCTGTCCAACCTCGAAGGCGGCCGTAGTCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGcc                                                                        >  1:228923/1‑148 (MQ=255)                                                                              ccTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACctgc                                                                 >  3:176126/1‑149 (MQ=255)                                                                               cTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACctgct                                                                <  5:353432/149‑1 (MQ=255)                                                                               cTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACctgct                                                                >  1:365119/1‑149 (MQ=255)                                                                                 gTCCAACCTCGAAGGCGCCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTgg                                                              >  5:384557/1‑149 (MQ=255)                                                                                  tCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCAGATCAACGAACACCAGAGCATTGCCAACCTGCTGGc                                                             >  7:211516/1‑149 (MQ=255)                                                                                    cAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                            >  3:75818/1‑148 (MQ=255)                                                                                    cAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                            >  7:327090/1‑148 (MQ=255)                                                                                     aaCCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                            <  4:65279/147‑1 (MQ=255)                                                                                     aaCCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                            >  3:65279/1‑147 (MQ=255)                                                                                       ccTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACa                                                        <  5:80400/149‑1 (MQ=255)                                                                                              ggcggcCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                            >  8:341248/1‑138 (MQ=255)                                                                                              ggcggcCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                            <  7:341248/138‑1 (MQ=255)                                                                                                   ccGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcaggtgcagg                                            <  5:156224/149‑1 (MQ=255)                                                                                                      tgggcGGCAATGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACct                                                                   <  5:36917/120‑1 (MQ=255)                                                                                                      tgggcGGCAATGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACct                                                                   >  6:36917/5‑124 (MQ=255)                                                                                                         tCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAAc                                       <  8:273820/149‑1 (MQ=255)                                                                                                           ggCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAAcgc                                     >  4:286232/1‑149 (MQ=255)                                                                                                              aTTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAAcgccgc                                  <  4:176126/149‑1 (MQ=255)                                                                                                                  ccgccCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGt                              >  8:324603/1‑149 (MQ=255)                                                                                                                  ccgccCAGGCCTTGGGCATCCCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGt                              <  8:327090/149‑1 (MQ=255)                                                                                                                    gccCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGttt                            <  8:334322/149‑1 (MQ=255)                                                                                                                     cccAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCaga                                                                                <  1:347580/96‑1 (MQ=255)                                                                                                                     cccAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCaga                                                                                >  2:347580/1‑96 (MQ=255)                                                                                                                      ccAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGc                          <  3:286232/149‑1 (MQ=255)                                                                                                                             ttGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCt                   >  3:73787/1‑149 (MQ=255)                                                                                                                                ggCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGc                 <  3:95751/148‑1 (MQ=255)                                                                                                                                          gcgcCGC‑TTCGAATGCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGACGACATGCAGGTCCAGGTGAACGCCGCACGTTTGCTGAACCTGCATGccgccccc      >  4:218286/1‑147 (MQ=255)                                                                                                                                              cgcATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTgc  <  4:75818/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGC  >  NC_002947/3962433‑3962721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑