Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,962,582:1	+T	7.4%	coding (780/1152 nt)	PP_3492 →	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,962,582	1	.	T	7.4%	263.0 / 15.6	68	coding (780/1152 nt)	PP_3492	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (28/35);  new base T (2/3);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

GCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCGCAG  >  NC_002947/3962434‑3962725                                                                                                                                                       |                                                                                                                                                gcgcGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGC‑TTCGaat                                                                                                                                                 >  3:258662/1‑148 (MQ=255)  cgcgGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑g                                                                                                                                                >  5:374622/1‑149 (MQ=255)     ggTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑gcgg                                                                                                                                             <  1:62559/149‑1 (MQ=255)         aCCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCt                                                                                                                                         >  6:333890/1‑149 (MQ=255)         aCCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCt                                                                                                                                         >  6:353432/1‑149 (MQ=255)         aCCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGC‑TTCGAATGCGgcgc                                                                                                                                          <  5:315173/148‑1 (MQ=255)           cTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGC‑TTCGAATGCGGCGCggg                                                                                                                                       <  4:258662/149‑1 (MQ=255)              cGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGt                                                                                                                                      <  2:90138/147‑1 (MQ=255)                 cAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCgggcgat                                                                                                                                   <  6:90962/147‑5 (MQ=255)                 cAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCgggcgat                                                                                                                                   >  5:90962/1‑143 (MQ=255)                    cTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTtctc                                                                                                                                <  6:151052/147‑1 (MQ=255)                     tGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTcgcg                                                                                                                             >  6:156224/1‑149 (MQ=255)                     tGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTcgcg                                                                                                                             >  3:317265/1‑149 (MQ=255)                       ccGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTg                                                                                                                           >  6:80400/1‑149 (MQ=255)                         gCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGAc                                                                                                                         <  1:238812/149‑1 (MQ=255)                           aTTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCg                                                                                                                       <  8:179702/149‑1 (MQ=255)                            ttCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCg                                                                                                                       >  8:323631/1‑148 (MQ=255)                               cTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATa                                                                                                                   <  5:53234/149‑1 (MQ=255)                                 gccgccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCGGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACa                                                                                                                 >  5:321215/1‑149 (MQ=255)                                    gccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGtt                                                                                                              >  4:116291/1‑149 (MQ=255)                                      cAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTg                                                                                                            <  5:202340/149‑1 (MQ=255)                                       aaTATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCTGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTgg                                                                                                           <  2:238841/149‑1 (MQ=255)                                           tCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCaa                                                                                                        <  2:335270/148‑1 (MQ=255)                                           tCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAg                                                                                                       <  6:215954/149‑1 (MQ=255)                                             cTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGc                                                                                                      <  6:321215/148‑1 (MQ=255)                                             cTCCGTGAGCGTGGCAAGGGCCTGGCCATTGCCCGGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCATGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGc                                                                                                      <  8:141661/148‑1 (MQ=255)                                              tCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTgg                                                                                                           <  5:335426/142‑1 (MQ=255)                                              tCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTgg                                                                                                           >  6:335426/1‑142 (MQ=255)                                                ggTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGa                                                                                                   <  1:78119/148‑1 (MQ=255)                                                  tGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTc                                                                                                                                     <  7:267145/112‑1 (MQ=255)                                                  tGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTc                                                                                                                                     >  8:267145/1‑112 (MQ=255)                                                  tGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCa                                                                                                <  1:360980/149‑1 (MQ=255)                                                           cAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                              >  7:62404/1‑142 (MQ=255)                                                           cAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                              <  8:62404/142‑1 (MQ=255)                                                            aaGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCa                                                                                      <  7:208330/149‑1 (MQ=255)                                                            aaGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCa                                                                                      <  1:168827/149‑1 (MQ=255)                                                               ggCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCagag                                                                                   <  3:116291/149‑1 (MQ=255)                                                                gCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCagag                                                                                   >  2:257750/1‑148 (MQ=255)                                                                gCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATAGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCagag                                                                                   >  6:363326/1‑148 (MQ=255)                                                                    ggCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTg                                                                              >  7:273820/1‑149 (MQ=255)                                                                        aTTGCCCTGTCCAACCTCGAAGGCGGCCGTAGTCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGcc                                                                            >  1:228923/1‑148 (MQ=255)                                                                             ccTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACctgc                                                                     >  3:176126/1‑149 (MQ=255)                                                                              cTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACctgct                                                                    >  1:365119/1‑149 (MQ=255)                                                                              cTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACctgct                                                                    <  5:353432/149‑1 (MQ=255)                                                                                gTCCAACCTCGAAGGCGCCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTgg                                                                  >  5:384557/1‑149 (MQ=255)                                                                                 tCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCAGATCAACGAACACCAGAGCATTGCCAACCTGCTGGc                                                                 >  7:211516/1‑149 (MQ=255)                                                                                   cAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                                >  3:75818/1‑148 (MQ=255)                                                                                   cAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                                >  7:327090/1‑148 (MQ=255)                                                                                    aaCCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                                <  4:65279/147‑1 (MQ=255)                                                                                    aaCCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                                >  3:65279/1‑147 (MQ=255)                                                                                      ccTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACa                                                            <  5:80400/149‑1 (MQ=255)                                                                                             ggcggcCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                                >  8:341248/1‑138 (MQ=255)                                                                                             ggcggcCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                                <  7:341248/138‑1 (MQ=255)                                                                                                  ccGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcaggtgcagg                                                <  5:156224/149‑1 (MQ=255)                                                                                                     tgggcGGCAATGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACct                                                                       <  5:36917/120‑1 (MQ=255)                                                                                                     tgggcGGCAATGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACct                                                                       >  6:36917/5‑124 (MQ=255)                                                                                                        tCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAAc                                           <  8:273820/149‑1 (MQ=255)                                                                                                          ggCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAAcgc                                         >  4:286232/1‑149 (MQ=255)                                                                                                             aTTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAAcgccgc                                      <  4:176126/149‑1 (MQ=255)                                                                                                                 ccgccCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGt                                  >  8:324603/1‑149 (MQ=255)                                                                                                                 ccgccCAGGCCTTGGGCATCCCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGt                                  <  8:327090/149‑1 (MQ=255)                                                                                                                   gccCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGttt                                <  8:334322/149‑1 (MQ=255)                                                                                                                    cccAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCaga                                                                                    >  2:347580/1‑96 (MQ=255)                                                                                                                    cccAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCaga                                                                                    <  1:347580/96‑1 (MQ=255)                                                                                                                     ccAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGc                              <  3:286232/149‑1 (MQ=255)                                                                                                                            ttGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCt                       >  3:73787/1‑149 (MQ=255)                                                                                                                               ggCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGc                     <  3:95751/148‑1 (MQ=255)                                                                                                                                         gcgcCGC‑TTCGAATGCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGACGACATGCAGGTCCAGGTGAACGCCGCACGTTTGCTGAACCTGCATGccgccccc          >  4:218286/1‑147 (MQ=255)                                                                                                                                             cgcATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTgc      <  4:75818/149‑1 (MQ=255)                                                                                                                                                 ttCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCGCAg  <  5:363326/149‑1 (MQ=255)                                                                                                                                                 ttCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCGCAg  <  1:257750/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                GCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTA‑TCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCGCAG  >  NC_002947/3962434‑3962725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑