Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	979,594:1	+T	6.1%	coding (1044/1215 nt)	iscS‑I →	cysteine desulfurase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	979,594	1	.	T	6.1%	266.3 / 9.7	66	coding (1044/1215 nt)	iscS‑I	cysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base . (29/33);  new base T (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CCTGAACCTGAGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGTTG  >  NC_002947/979450‑979733                                                                                                                                                  |                                                                                                                                             cctgaacctgaGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTCCGTACTGCCTGCTCTGGGCCGCAACGACGAGCTG‑gcg                                                                                                                                           >  2:74370/1‑148 (MQ=255)  ctgaacctgaGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGca                                                                                                                                         <  3:235090/149‑1 (MQ=255)     accctgaTCTTTCACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGcac‑a                                                                                                                                      <  1:129620/146‑1 (MQ=255)     aacctgaGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑Ag                                                                                                                                     <  8:348753/149‑1 (MQ=255)           aGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTGTGCGC‑C‑AGCTCgt                                                                                                                                <  4:368315/148‑2 (MQ=255)            gCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTCCGTACTGCGTGCTCTGGGCCGCAACGACGAGGTGTGCGC‑C‑AGCTCgtcg                                                                                                                              <  1:87794/149‑4 (MQ=255)               tCAGCTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGc                                                                                                                           >  3:278834/1‑149 (MQ=255)               tCAACTACGTCGAAGGCGAATCGCTGCTTATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGc                                                                                                                           >  5:236944/1‑149 (MQ=255)               tCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGc                                                                                                                           >  6:379680/1‑149 (MQ=255)                  aCTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTtc                                                                                                                        <  5:47310/149‑1 (MQ=255)                  aCTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTtc                                                                                                                        <  3:334928/149‑1 (MQ=255)                     aCGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTtctc                                                                                                                      <  4:188421/148‑1 (MQ=255)                         cGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCg                                                                                                                 >  4:62844/1‑149 (MQ=255)                           aaGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGc                                                                                                               <  3:3569/149‑1 (MQ=255)                                         ctgATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCACAAGCTCGATCCGCTTCTCCTTCGGCCGTTTCaccacc                                                                                                   <  1:61595/148‑1 (MQ=255)                                              gTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCgaag                                                                                               >  6:226357/1‑146 (MQ=255)                                                 gCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGaagaagaag                                                                                         >  7:70212/1‑149 (MQ=255)                                                 gCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGaagaagaag                                                                                         >  3:291009/1‑149 (MQ=255)                                                 gCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGaagaagaag                                                                                         >  8:184524/1‑149 (MQ=255)                                                  cTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGt                                                                                        <  7:184524/149‑1 (MQ=255)                                                     aaaGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGa                                                                                     <  5:223197/149‑1 (MQ=255)                                                           aTCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTAcgc                                                                               >  3:338924/1‑149 (MQ=255)                                                           aTCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTAcgc                                                                               >  2:353938/1‑149 (MQ=255)                                                            tCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCTCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGttt                                                                                                          <  7:55665/121‑1 (MQ=255)                                                            tCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGttt                                                                                                          >  8:55665/1‑121 (MQ=255)                                                              gCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTAcgccgc                                                                            <  6:236944/149‑1 (MQ=255)                                                                      gTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGaaa                                                                     <  5:339460/148‑1 (MQ=255)                                                                      gTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGaaa                                                                     <  5:283987/148‑1 (MQ=255)                                                                      gTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGa                                                                       >  6:283987/1‑146 (MQ=255)                                                                      gTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAg                                                                    >  2:195097/1‑149 (MQ=255)                                                                           gTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGc                                                               <  7:39148/149‑1 (MQ=255)                                                                           gTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGc                                                               <  3:62844/149‑1 (MQ=255)                                                                            ttCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGc                                                               >  1:15488/1‑148 (MQ=255)                                                                               ggCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGACTTTCTGCgcg                                                            <  2:186508/148‑3 (MQ=255)                                                                               ggCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGATTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGatg                                                           <  8:223207/149‑3 (MQ=255)                                                                                 ccTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGcc                                                         <  1:306259/149‑1 (MQ=255)                                                                                    gTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGt                                                       >  7:4693/1‑148 (MQ=255)                                                                                     tACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGt                                                       <  1:353938/147‑1 (MQ=255)                                                                                        cTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAAt                                                   >  1:11941/1‑148 (MQ=255)                                                                                           cgcggggCTCGAGCCGTCGTAAGTACTGCTTGCTGTGGGCCGCTACGACGAGCTG‑GCGCTC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCTCCACCGAAGAAGAAGTCGACTACGCCGCGCAGATAGTCTGCGAGGCCGTCAATaat                                                <  1:74370/142‑2 (MQ=255)                                                                                           cgcgTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAAc                                               >  2:40608/1‑149 (MQ=255)                                                                                               tCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCg                                           >  8:116732/1‑149 (MQ=255)                                                                                                 gCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCg                                           <  4:345334/147‑1 (MQ=255)                                                                                                 gCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCg                                           >  3:345334/1‑147 (MQ=255)                                                                                                 gCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGt                                          <  4:278834/148‑1 (MQ=255)                                                                                                  cTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGa                                        <  8:70212/149‑1 (MQ=255)                                                                                                  cTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGa                                        <  8:4693/149‑1 (MQ=255)                                                                                                    cGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGc                                      >  7:200522/1‑149 (MQ=255)                                                                                                     gAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTGTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCt                                     >  6:3920/1‑149 (MQ=255)                                                                                                        ccGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTc                                  >  1:37285/1‑149 (MQ=255)                                                                                                        ccGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTc                                  <  5:226357/149‑1 (MQ=255)                                                                                                          gTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTcgc                                <  1:40608/149‑1 (MQ=255)                                                                                                                 gTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCtgtg                         >  5:108586/1‑149 (MQ=255)                                                                                                                  tACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTgggg                        <  6:108586/149‑1 (MQ=255)                                                                                                                   aCTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCtgtg                         <  5:217531/147‑1 (MQ=255)                                                                                                                   aCTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCtgtg                         >  6:217531/1‑147 (MQ=255)                                                                                                                       cGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGata                    <  5:379680/148‑1 (MQ=255)                                                                                                                            tctGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGatat                   <  6:96902/144‑1 (MQ=255)                                                                                                                            tctGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGatat                   >  5:96902/1‑144 (MQ=255)                                                                                                                            tctGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTAc               >  8:256793/1‑148 (MQ=255)                                                                                                                              tGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACa              <  7:116732/147‑1 (MQ=255)                                                                                                                                ggCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGa          >  3:357666/1‑149 (MQ=255)                                                                                                                                     cAACCACCAGCTG‑GCCCAC‑AGCTCGATCCGCTTCTCCTTCGGCCCTTTCACCACCCAAGAAGAACACGACTACGCCGCGCAGAAAGTCACCGAGGCCGGCAATAAACTGCGTGAGCTCTCGCAGCTGTGGCATATGTACAAACACGGCg     >  8:277315/1‑149 (MQ=255)                                                                                                                                      aaCGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGt    >  3:68677/1‑149 (MQ=255)                                                                                                                                        gatcgaGCTGTGCGC‑C‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCTAGAAGAAAGTCTGCGAGTAAGTCAATAAAATGCGTGAGATGTCGACGCTGTGGTATATGTACAAAGACGGAGtt   >  5:185734/4‑148 (MQ=255)                                                                                                                                        gatcgaGCTGTGCGC‑C‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGtt   >  7:155761/4‑148 (MQ=255)                                                                                                                                        cgacgaGCTG‑GCGCAC‑AGGTGGTTCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGTAGAAGTCGACTACGCCGGGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTGGCCGCTGTGGGATATGTACAAAGACGGCGTTg  <  8:200522/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                             CCTGAACCTGAGCTTCAACTACGTCGAAGGCGAATCGCTGCTGATGTCGCTGAAAGACATCGCCGTATCGTCCGGTTCGGCCTGTACCTCCGCGTCGCTCGAGCCGTCCTACGTACTGCGTGCTCTGGGCCGCAACGACGAGCTG‑GCGCAC‑AGCTCGATCCGCTTCTCCTTCGGCCGTTTCACCACCGAAGAAGAAGTCGACTACGCCGCGCAGAAAGTCTGCGAGGCCGTCAATAAACTGCGTGAGCTGTCGCCGCTGTGGGATATGTACAAAGACGGCGTTG  >  NC_002947/979450‑979733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑