| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,126,645:1 | +C | 100% | intergenic (‑99/+50) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 88.9% | 102.5 / 8.4 | 36 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base C (11/21); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAA‑CC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947/1126500‑1126788 | gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAA‑ccc > 2:64023/1‑148 (MQ=255) aGGGTCCGATGCCGTTCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCGAGGGTGTGGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTGTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAA‑cccg < 6:160564/149‑3 (MQ=38) aGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAA‑ccc < 1:64023/148‑2 (MQ=255) aGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTCGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAA‑cccg < 5:237768/149‑3 (MQ=255) cAGGGCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCAcc < 3:29839/149‑1 (MQ=255) gCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTAACCACCAATGCGGAGGTATATACCCCAAACCAATACCC‑GGT‑AAAA‑AC‑CCACCAAAACAAAG‑CCCGCCACCccagccagcggg > 4:266649/1‑145 (MQ=255) aGTGACTTGGGGACCTCGTGCCTGTTTGTTGGGGGTACTTTGCGCCGTGTCGGCGG‑GGGTTTCCCCGCTATTGCGGAGTTATC‑GCCACCATCGCTTTCGG‑GGT‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCg < 2:183885/147‑1 (MQ=255) aCATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAA‑CCTGCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAgg < 7:105890/149‑1 (MQ=255) gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGGTTTACCC‑CCAATGGGGGGGGATA‑GCCCCCCACGGCTTAGCGGGG‑AAAA‑CC‑CCAGCAACACAGAGCCCCCCCACCCCACCCCGggggggggggggcg > 6:319804/1‑146 (MQ=255) aCCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg < 2:321779/149‑1 (MQ=255) ctcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGc < 6:106524/149‑1 (MQ=255) tGCCAGGTTGTTGGGTGGAATTTGAGCCCTGTAGGCGC‑GGGTTTACCCGCTAATGCGTAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGt < 3:12540/125‑1 (MQ=25) ccggTTGTGGGGGGGACATGGCGCCCTGTAGCCGC‑GGGTTAACCCGCGAATGCGGAGGTATA‑CCCACCATCGCATTCGC‑GGT‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 5:45630/147‑1 (MQ=255) cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 1:42950/149‑1 (MQ=255) cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTAACCGCCAATTCGGAGGTTTA‑TCCACCAAAGCATTATC‑GGGTTAAA‑CC‑GCGCCTACACAGAG‑CCCGCCCCCCCACCCACGTGGCGGGGAGGGCTATAAGGCGTGCTGa > 1:22624/1‑149 (MQ=255) aGGTGGTTGGGTGGACTTTGCGCCCTGAAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATC‑GCCACCATCGCATTCGC‑GGT‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt < 7:134201/149‑1 (MQ=37) gttgttGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGCCGGGGAGGGCTATCAGGACGCCTGATAg < 1:213596/149‑1 (MQ=37) cgatctgGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt < 1:248478/142‑1 (MQ=255) ttgGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctc < 5:319804/148‑1 (MQ=255) gTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcgat < 7:86870/149‑1 (MQ=255) cccTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc < 5:309121/149‑1 (MQ=255) gTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACaga > 7:259462/1‑65 (MQ=255) gTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACaga < 8:259462/65‑1 (MQ=255) ttACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCgg > 2:201069/1‑149 (MQ=255) cccGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAg > 3:172634/1‑139 (MQ=255) cccGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAg < 4:172634/139‑1 (MQ=255) ccGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCGCACCAGGTTGCGGTCGc > 4:340464/1‑149 (MQ=255) ccGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCgg > 1:223490/1‑148 (MQ=255) ccGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc < 3:266649/149‑1 (MQ=255) gcgAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc > 1:289937/1‑148 (MQ=255) gCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGTTGAGGGCTATCAGGCGTCATGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCgg < 5:262048/137‑1 (MQ=39) ggAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt > 4:339499/1‑149 (MQ=255) ggTATA‑GCCACCATCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTc < 3:123025/149‑1 (MQ=255) caTCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGa < 7:261107/149‑1 (MQ=255) caTCGCATTCGC‑GGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGa < 1:252751/149‑1 (MQ=255) c‑gGG‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg > 6:364172/1‑149 (MQ=255) ggg‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg > 8:299823/1‑148 (MQ=255) g‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAc > 5:289344/1‑149 (MQ=255) g‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAc > 7:280130/1‑149 (MQ=255) g‑TAAACCC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAc < 2:22624/149‑1 (MQ=255) | GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATA‑GCCACCATCGCATTCGC‑GGG‑TAAA‑CC‑GCTCCTACACAGAG‑CCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947/1126500‑1126788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |