Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,419,548:1 +C 100% intergenic (‑37/+99) PP_1242 ← / ← PP_1244 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,419,5481.C97.0% 104.2 / ‑3.2 33intergenic (‑37/+99)PP_1242/PP_1244hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (16/16);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATG  >  NC_002947/1419401‑1419679
                                                                                                                                                     |                                                                                                                                   
cTGGCAGGGGATGCCGGGTTCGATGGGCAACAGCGGGGGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggc‑tgg‑c                                                                                                                                    <  1:16565/149‑1 (MQ=255)
cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTTGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTGGCCTGggc‑tgg‑c                                                                                                                                    <  3:236714/149‑1 (MQ=255)
cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggc‑tgg‑c                                                                                                                                    >  5:295330/1‑149 (MQ=255)
cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggc‑tgg‑c                                                                                                                                    <  2:335586/149‑1 (MQ=255)
cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggc‑tgg‑c                                                                                                                                    <  3:84236/149‑1 (MQ=255)
         gATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTcgcg                                                                                                                            >  7:40660/1‑149 (MQ=255)
               gggTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACg                                                                                                                      >  4:185904/1‑149 (MQ=255)
                  ttCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGccc                                                                                                                   <  8:40660/149‑1 (MQ=255)
                      aTGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTc                                                                                                               >  7:88570/1‑149 (MQ=255)
                         cGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCca                                                                                                            <  3:185904/149‑1 (MQ=255)
                            aaCAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                         <  3:90134/149‑1 (MQ=255)
                             aCAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAgg                                                                                                        >  1:127696/1‑149 (MQ=255)
                             aCAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑CGGCCTCTTCGCGGGCACGCCCGCTCCCACAgg                                                                                                        >  3:39199/1‑149 (MQ=255)
                               aGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGt                                                                                                       >  8:310322/1‑148 (MQ=255)
                               aGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTa                                                                                                      <  3:161869/149‑1 (MQ=255)
                                 cGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                    <  2:127696/149‑1 (MQ=255)
                                 cGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑CGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                    <  4:39199/149‑1 (MQ=255)
                                    gTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACtgtg                                                                                                 <  6:295330/149‑1 (MQ=255)
                                    gTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGAATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACtgtg                                                                                                 <  5:333041/149‑1 (MQ=255)
                                       aGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGTTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCa                                                                                               <  2:285672/148‑1 (MQ=255)
                                             gttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGt                                                                                         <  4:307875/148‑1 (MQ=255)
                                                     cTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTTGGCTTGC‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgt                                                                                <  6:279020/149‑1 (MQ=255)
                                                         gTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                    <  4:1567/125‑1 (MQ=255)
                                                         gTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                    >  3:1567/1‑125 (MQ=255)
                                                         gTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                    >  7:318608/1‑125 (MQ=255)
                                                               gCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtg                                                                      >  8:125242/1‑149 (MQ=255)
                                                                 aGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGTCCCtgtg                                                                    >  4:252977/1‑149 (MQ=255)
                                                                 aGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtgtg                                                                    <  7:310322/149‑1 (MQ=255)
                                                                         ccGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCgg                                                             <  7:125242/148‑1 (MQ=255)
                                                                                          tgttgtgttgTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGGAGTGCGTGTCGGCGTTGGTGACCCAGTGGGACCCGGCGTGCCCGCGAAGAGGc                                           >  7:185295/1‑149 (MQ=38)
                                                                                             tgtgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGGAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCt                                         >  1:49599/1‑148 (MQ=255)
                                                                                              gtgttggCGGGGGGCATTGTTTGGTTGCCCTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCCGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGa                                       <  8:88570/149‑1 (MQ=37)
                                                                                                                         cccTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAgg                        >  5:103124/1‑137 (MQ=255)
                                                                                                                         cccTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCtg            >  5:193745/1‑149 (MQ=255)
                                                                                                                         cccTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGGGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAgg                        <  6:103124/137‑1 (MQ=255)
                                                                                                                           cTGGATTCTGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGGAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTTATGCAAGGTGTGAGGCTCtgtg          >  1:214357/1‑149 (MQ=255)
                                                                                                                              caTTCGGGGTGGCCTGGGC‑TGGCCGCTTCGCGGCCACGCCCGCCCCCACAGGTACTGTGCAGTGCGGGACGGCGGTGGTCCCCCGGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGt       <  2:214357/148‑1 (MQ=37)
                                                                                                                                   tGGGTTGCCTGGGC‑TGGCCTCTTCGCGGGCACCCCCGCACCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCGGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGGCCTTCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATg  >  2:11386/1‑149 (MQ=38)
                                                                                                                                                     |                                                                                                                                   
CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGC‑TGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATG  >  NC_002947/1419401‑1419679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑