Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,915,598:1	+A	9.2%	intergenic (+26/‑263)	fklB‑II → / → PP_1715	FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,915,598	1	.	A	9.2%	243.7 / 16.1	65	intergenic (+26/‑263)	fklB‑II/PP_1715	FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (33/26);  new base A (3/3);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CCGCTGATCCCGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAA  >  NC_002947/1915450‑1915746                                                                                                                                                      |                                                                                                                                                     ccGCTGATCCCGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGcccc                                                                                                                                                       <  2:117100/149‑1 (MQ=255)      gATCCCGGCCAACTCGGTACTGGTTTTCGATCTGGAACTGATAGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCAGCAGGCCGAAGACACTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACAtt                                                                                                                                                 >  7:117441/1‑149 (MQ=255)          ccGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACAttgttg                                                                                                                                             >  4:180698/1‑149 (MQ=255)          ccGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACAttgttg                                                                                                                                             >  7:171065/1‑149 (MQ=255)           cGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTgg                                                                                                                                            >  7:113398/1‑149 (MQ=255)              ccAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCAGCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTggg                                                                                                                                           <  7:293503/147‑1 (MQ=255)                  cTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGttt                                                                                                                                     <  3:287060/149‑1 (MQ=255)                  cTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGttt                                                                                                                                     >  3:169988/1‑149 (MQ=255)                   tCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATAGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGtttt                                                                                                                                    >  3:7321/1‑149 (MQ=255)                    cGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTc                                                                                                                                   >  5:28093/1‑149 (MQ=255)                     ggTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGAAAACGCCCCAACAATG‑TGGGGCGtttcc                                                                                                                                   <  1:328552/148‑3 (MQ=255)                            gtgtTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTtgtg                                                                                                                            <  8:11472/148‑1 (MQ=255)                            gtgtTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTtgt                                                                                                                             <  5:346176/147‑1 (MQ=255)                                       ggAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACg                                                                                                                 >  6:99344/1‑148 (MQ=255)                                               tCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCaa                                                                                                        <  4:263675/149‑1 (MQ=255)                                               tCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCaa                                                                                                        >  3:140912/1‑149 (MQ=255)                                                  ccATCAAGGACCCGGCCCAGTTGCACGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCTGAAACGACCC‑ACATTGTTTGGGCGTTTTCGTTTGTGCCGAtt                                                                                                                      >  8:150719/1‑132 (MQ=255)                                                  ccATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCGC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAg                                                                                                     <  8:113398/149‑1 (MQ=255)                                                  ccATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCCAACAATG‑TGGGGCGtt                                                                                                                                      <  4:123484/116‑1 (MQ=255)                                                  ccATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCCAACAATG‑TGGGGCGtt                                                                                                                                      >  3:123484/1‑116 (MQ=255)                                                   cATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGaaa                                                                                                        >  6:244408/1‑145 (MQ=255)                                                       aaGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGa                                                                                                 >  6:249643/1‑148 (MQ=255)                                                       aaGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGa                                                                                                 <  5:99344/148‑1 (MQ=255)                                                         ggACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAAc                                                                                                              >  6:177666/1‑133 (MQ=255)                                                         ggACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAAc                                                                                                              <  5:177666/133‑1 (MQ=255)                                                            cccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGt                                                                                           >  4:106632/1‑149 (MQ=255)                                                            cccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGt                                                                                           <  6:28093/149‑1 (MQ=255)                                                            cccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGt                                                                                           >  5:321249/1‑149 (MQ=255)                                                            cccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGt                                                                                           >  6:163683/1‑149 (MQ=255)                                                               ggCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTTGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTcc                                                                                         >  6:175158/1‑148 (MQ=255)                                                                gCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGGTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTcc                                                                                         >  1:336582/1‑147 (MQ=255)                                                                 cccAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTTCAACAGCTGATACTGTCCGAg                                                                                      <  4:7321/149‑1 (MQ=255)                                                                  ccAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAg                                                                                      >  6:326396/1‑148 (MQ=255)                                                                   cAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAgg                                                                                     >  1:204032/1‑148 (MQ=255)                                                                        gCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGc                                                                               >  3:195685/1‑149 (MQ=255)                                                                         cAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTTGGGCGTTTTCGTTTGTGCCGATTGTACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCa                                                                              <  7:40364/149‑1 (MQ=255)                                                                          aaGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAg                                                                             <  4:195685/149‑1 (MQ=255)                                                                          aaGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAg                                                                             <  8:171065/149‑1 (MQ=255)                                                                          aaGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACAATGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAg                                                                             <  8:117441/149‑1 (MQ=255)                                                                                  gAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaag                                                                      >  4:292946/1‑148 (MQ=255)                                                                                     ccGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGt                                                                  <  3:180698/149‑1 (MQ=255)                                                                                      cGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTa                                                                 <  5:326396/149‑1 (MQ=255)                                                                                      cGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTa                                                                 <  5:244408/149‑1 (MQ=255)                                                                                                 gCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCt                                                       >  5:174873/1‑148 (MQ=255)                                                                                                     cTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGc                                                  <  3:106632/149‑1 (MQ=255)                                                                                                         agaagCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCaaa                                               <  6:174873/148‑1 (MQ=255)                                                                                                          gaagCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATc                                             <  4:169988/149‑1 (MQ=255)                                                                                                                  tGCCAAGTAACATTGGCTGGATGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTCAAGAAGTAGGATCTTTCTGACGCAAATCGTTTgcgc                                     >  5:95853/1‑149 (MQ=255)                                                                                                                    ccAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTgcgc                                     <  5:249643/147‑1 (MQ=255)                                                                                                                    ccAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGcgccg                                   >  5:373501/1‑149 (MQ=255)                                                                                                                      aaGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaagaa                                                                    <  4:320287/114‑1 (MQ=255)                                                                                                                      aaGTAACATTGGATGGTTGTCGGAAAAGCCAC‑ACATTGTTGGGGAGATTTCGTTTGAGCCGATTGGAAGAACATGCAACAGCATATACTGTCCGAGGAAATGCAGCGTGAACAAGTAGAATCTTTCTGAAGCAAATCGTTTGcgccg                                   >  6:240406/1‑147 (MQ=255)                                                                                                                         tAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAACGATTTgcgtcagaaa                               >  1:114944/1‑141 (MQ=255)                                                                                                                                 gatGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGTACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGAAGCAAATCGTTTGAGCCGCCGGTAGTCAttg                      >  3:44870/3‑147 (MQ=255)                                                                                                                                  cTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGc                     >  3:91518/1‑149 (MQ=255)                                                                                                                                     gTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGt                   >  1:232343/1‑148 (MQ=255)                                                                                                                                      ttGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGa                 <  5:11735/149‑1 (MQ=255)                                                                                                                                           ggAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGa            >  7:221670/1‑149 (MQ=255)                                                                                                                                            gAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGt                                                                  <  6:3029/94‑1 (MQ=255)                                                                                                                                            gAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGt                                                                  >  5:3029/1‑94 (MQ=255)                                                                                                                                            gAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGAc           >  5:232262/1‑149 (MQ=255)                                                                                                                                            aaaaCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGAGGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCa                         >  1:190572/2‑135 (MQ=255)                                                                                                                                             aaaCGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACt          <  5:175158/149‑1 (MQ=255)                                                                                                                                             aaaCGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACt          <  8:221670/149‑1 (MQ=255)                                                                                                                                               aCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGAc           <  6:332532/146‑1 (MQ=255)                                                                                                                                               aCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGAc           >  5:332532/1‑146 (MQ=255)                                                                                                                                                     c‑acaTTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTaaaa  <  5:163683/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CCGCTGATCCCGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAA  >  NC_002947/1915450‑1915746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑