| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,932,638:1 | +C | 100% | intergenic (‑105/+45) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 92.1% | 115.3 / 3.9 | 38 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (20/15); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑G‑CCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932499‑1932776 | tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAg < 4:289202/149‑1 (MQ=255)tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAg < 1:292762/149‑1 (MQ=255) gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCACCTAAAAAGGATATGCGTCCCCGGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGcgg > 1:191973/1‑147 (MQ=38) gcatGGTCATTCGACAGCGGCATTGCAGGTCTCACTAAAATCGCCGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGTAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAgg < 6:307233/144‑1 (MQ=37) tCTGGACATTAGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCAGCTAGAAATGAATTGGGGCCCCAGTAGTAGCGGCCT‑T‑GCCCGCCTAGGGCAGC‑AACGCac > 8:38344/1‑148 (MQ=255) cATTCGACAGCGGCATTCCAGGGCTCACGAAAATCGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCAACAAAGCATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCaa < 6:120362/149‑1 (MQ=37) tgccaccgCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGGGGCCCGTCGCGGCCAAGCCCGCTCCTCCACCGGATCTGCGTCCCCTGTAGGCGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAAt < 5:65926/142‑1 (MQ=37) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGAGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTc > 5:311485/1‑149 (MQ=255) aGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAACGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAAATGTcca > 1:222507/1‑147 (MQ=255) tctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAATGGCTGCTAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 1:239216/149‑1 (MQ=38) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTACCCAGTTGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑CAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 8:325760/1‑149 (MQ=38) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg > 1:252563/1‑149 (MQ=255) acaGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGCATATGCGTCCCCTTTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAg < 5:326968/147‑1 (MQ=255) ggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa < 4:292415/149‑1 (MQ=255) cGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑TGG‑CCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCCGCTCCAGa > 1:339318/1‑148 (MQ=255) cTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc > 5:66247/1‑149 (MQ=255) cTTGGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAATGTATCTCCGTCCCCTGTCGGCGGGGGGT‑TGG‑CCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 2:339318/149‑1 (MQ=255) cgcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt > 7:334672/1‑149 (MQ=255) cgcgGCCACCCCCGCTCCTACACAGTATCTGCGCCCCCGGTCGGCGGGGGCTGC‑C‑CCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt < 2:358808/149‑1 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 4:191447/1‑149 (MQ=255) ccgggtCCAACAATGGTTATGGGTCCGGTGTAGGTGGGGGGT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAATTTGTCGACTACGCGGATCAGCGTTCCGGCAGGGTGATGTTCAGGTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa < 7:38344/143‑1 (MQ=255) cccTCCTACAAAGGAAATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt < 8:244237/147‑1 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc > 8:182538/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 3:122598/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 7:81718/1‑149 (MQ=255) aaGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 2:241325/1‑148 (MQ=255) atGCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc > 2:134112/1‑149 (MQ=255) gCGTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 7:35961/1‑149 (MQ=255) cGTCCCCTGTTGCTGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTTCGCGGATCAGCGATCCGGCATGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTTGTTTTCCAGCTCGATATGCACATGATCttt < 6:311485/149‑1 (MQ=255) gTCCCCTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCATCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg < 6:66247/149‑1 (MQ=255) cccccTGTCGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 5:7973/148‑1 (MQ=255) cccTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGACTCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGGCg > 4:30342/1‑149 (MQ=255) cccTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc > 5:14012/1‑148 (MQ=255) ccTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt > 3:57563/1‑144 (MQ=255) ccTGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt < 4:57563/144‑1 (MQ=255) tGTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATg > 4:312106/1‑149 (MQ=255) gTAGGAGCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt > 7:310987/1‑149 (MQ=255) gCGGGCT‑T‑GCCCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca < 1:318055/148‑1 (MQ=255) | TCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCT‑T‑G‑CCGCGAAGGGCTGC‑AAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932499‑1932776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |