Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,042,436:1	+A	8.0%	coding (120/1002 nt)	PP_1816 ←	alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,042,436	1	.	A	8.0%	287.7 / 19.1	75	coding (120/1002 nt)	PP_1816	alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (34/35);  new base A (4/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

GGCTGGTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTGGAG  >  NC_002947/2042290‑2042579                                                                                                                                                    |                                                                                                                                                ggCTGGTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑Ac                                                                                                                                               >  8:47073/1‑149 (MQ=255) ggCTGGTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑Ac                                                                                                                                               <  4:95582/149‑1 (MQ=255)  gcgggTAGCCGGGGGGCTTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑Acc                                                                                                                                              <  1:292831/146‑1 (MQ=255)      gTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGt                                                                                                                                           <  6:82972/148‑1 (MQ=255)      gTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGt                                                                                                                                           <  6:77637/148‑1 (MQ=255)       tAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTAc                                                                                                                                         >  2:316940/1‑149 (MQ=255)       tAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTAc                                                                                                                                         >  3:133097/1‑149 (MQ=255)                  aTTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGc                                                                                                                              >  8:241268/1‑149 (MQ=255)                      gAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCggg                                                                                                                          <  4:232574/149‑1 (MQ=255)                        ggCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGgtt                                                                                                                        <  4:66094/149‑1 (MQ=255)                         gCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGgttg                                                                                                                       >  3:212053/1‑149 (MQ=255)                           aaCCACTTCACCTACACGCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGgttgtt                                                                                                                     <  8:83538/149‑1 (MQ=255)                           aaCCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGgttgtt                                                                                                                     >  4:174301/1‑149 (MQ=255)                           aaCCACTTCACCTACACCCAGCGCACGCATTACATGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATCGCCGGGTCCAGCGACAGGTACAGGATGCGC‑ACCAGTACCTGGCCTTCGCCGGgttgtg                                                                                                                     >  7:79498/1‑148 (MQ=255)                                   cACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCggg                                                                                                              <  7:47073/148‑1 (MQ=255)                                      cTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACg                                                                                                          <  4:133097/149‑1 (MQ=255)                                           cccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGtctc                                                                                                     >  4:25848/1‑149 (MQ=255)                                            ccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCgg                                                                                                                           >  5:20139/1‑126 (MQ=255)                                            ccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCgg                                                                                                                           <  6:20139/126‑1 (MQ=255)                                            ccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGtctc                                                                                                     >  4:314309/1‑148 (MQ=255)                                            ccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGtctc                                                                                                     >  3:52964/1‑148 (MQ=255)                                              aGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGaa                                                                                                  <  7:100346/149‑1 (MQ=255)                                               gcgcACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCCGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAg                                                                                                 >  2:51417/1‑149 (MQ=255)                                                cgcacgcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGc                                                                                                >  3:132699/1‑149 (MQ=255)                                                 gcacgcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCt                                                                                               >  3:212690/1‑149 (MQ=255)                                                   acgcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTGGCCTTCGTTGATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACATGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGa                                                                                             <  8:327608/149‑1 (MQ=255)                                                    cgcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGaa                                                                                            <  4:364583/149‑1 (MQ=255)                                                     gcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAg                                                                                           >  5:235793/1‑149 (MQ=255)                                                          aCCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAgtcgt                                                                                       <  1:316940/148‑1 (MQ=255)                                                           ccTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAACCTGAAgtcgtcc                                                                                     >  7:195017/1‑148 (MQ=255)                                                            cTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGTGGGTACGGTCTCGAAGCTGAAGTCGTcac                                                                                    >  5:334644/1‑149 (MQ=255)                                                            cTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcac                                                                                    >  5:366771/1‑149 (MQ=255)                                                            cTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcac                                                                                    >  5:124793/1‑149 (MQ=255)                                                              ggCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacgg                                                                                  >  5:218337/1‑149 (MQ=255)                                                               gCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacggc                                                                                 >  1:210244/1‑149 (MQ=255)                                                                cccAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacggcg                                                                                >  7:47081/1‑149 (MQ=255)                                                                 ccAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacggcgc                                                                               >  2:78417/1‑149 (MQ=255)                                                                  cAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacggcgca                                                                              >  6:42479/1‑149 (MQ=255)                                                                         aCGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGcc                                                                       <  8:47081/149‑1 (MQ=255)                                                                             gcgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtga                                                                   <  7:241268/149‑4 (MQ=255)                                                                             gcgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtga                                                                   <  3:314309/149‑4 (MQ=255)                                                                             gcgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtga                                                                   <  1:78417/149‑4 (MQ=255)                                                                              cgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtgac                                                                  <  4:105117/149‑5 (MQ=255)                                                                              cgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtgac                                                                  <  4:52964/149‑5 (MQ=255)                                                                              cgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCg                                                                  <  6:235793/149‑1 (MQ=255)                                                                               gggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCgg                                                                 >  6:207724/1‑149 (MQ=255)                                                                                ggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCggg                                                                <  4:132699/149‑1 (MQ=255)                                                                                 gATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGc                                                               <  6:218337/149‑1 (MQ=255)                                                                                     tAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGttt                                                           <  3:174301/149‑1 (MQ=255)                                                                                       ggACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTg                                                          <  6:124793/148‑1 (MQ=255)                                                                                        gACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTgg                                                         >  7:36198/1‑148 (MQ=255)                                                                                        gACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTgg                                                         >  4:288124/1‑148 (MQ=255)                                                                                         aCTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTTCGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCTGGCGTTTGGCa                                                       <  4:212053/149‑1 (MQ=255)                                                                                               cTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAgg                                                 <  6:334644/149‑1 (MQ=255)                                                                                                   gTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAg                                              <  4:212690/148‑1 (MQ=255)                                                                                                      cATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCCGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAgcgg                                           <  1:51417/148‑1 (MQ=255)                                                                                                       aTCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGa                                                                     <  8:306196/121‑1 (MQ=255)                                                                                                       aTCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGa                                                                     >  7:306196/1‑121 (MQ=255)                                                                                                        tCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGcggcgg                                        <  5:207724/149‑1 (MQ=255)                                                                                                           aGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTg                                     <  3:25848/149‑1 (MQ=255)                                                                                                                    tGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTc                            <  6:366771/149‑1 (MQ=255)                                                                                                                           gTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCgg                     <  8:36198/149‑1 (MQ=255)                                                                                                                              cAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTc                  >  3:221943/1‑149 (MQ=255)                                                                                                                              cAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGCCGGTTGGTCTGGTTCATGTCGGGTc                  >  2:36533/1‑149 (MQ=255)                                                                                                                               aGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTcc                 >  8:166571/1‑149 (MQ=255)                                                                                                                               aGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTcc                 >  3:202129/1‑149 (MQ=255)                                                                                                                                 ggccaggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGTGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCtt               >  8:330972/4‑149 (MQ=255)                                                                                                                                 ggccaggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCtt               >  5:129859/4‑149 (MQ=255)                                                                                                                                    caggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTgg                               >  2:368496/1‑130 (MQ=255)                                                                                                                                    caggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTgg                               <  1:368496/130‑1 (MQ=255)                                                                                                                                    caggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGCCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTtgtg            >  8:111326/1‑149 (MQ=255)                                                                                                                                         acaggtTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCgg       >  8:60123/1‑149 (MQ=255)                                                                                                                                           aggtTGCGC‑AGCAGTTCCTGGCCTGCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTg     <  8:195017/149‑1 (MQ=255)                                                                                                                                             gtTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTg     >  2:136529/1‑147 (MQ=255)                                                                                                                                               tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTGGAg  <  6:129859/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                GGCTGGTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTGGAG  >  NC_002947/2042290‑2042579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑