Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,141,507:1 +A 10.4% coding (424/972 nt) PP_2757 → sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,141,5071.A10.4% 168.0 / 16.3 48coding (424/972 nt)PP_2757sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (23/20);  new base A (2/3);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAG  >  NC_002947/3141362‑3141653
                                                                                                                                                  |                                                                                                                                                  
ggTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGt                                                                                                                                                 >  5:300045/1‑149 (MQ=255)
ggTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGt                                                                                                                                                 >  8:156541/1‑149 (MQ=255)
 gTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGtt                                                                                                                                                >  5:247816/1‑149 (MQ=255)
  tAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTg                                                                                                                                               <  7:156541/149‑1 (MQ=255)
    aTTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACG‑CGCGGCCGGCCGCGACGTTgtc                                                                                                                                             <  6:163284/149‑3 (MQ=255)
    aTTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGc                                                                                                                                             <  6:300045/149‑1 (MQ=255)
        aGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACG‑CGCGGCCGGCCGCGACGTTGTcc                                                                                                                                            >  4:17677/1‑146 (MQ=255)
        aGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACG‑CGCGGCCGGCCGCGACGTTGTcc                                                                                                                                            <  3:17677/146‑1 (MQ=255)
            ccTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGc                                                                                                                                     <  6:306216/149‑1 (MQ=255)
            ccTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGc                                                                                                                                     >  8:166188/1‑149 (MQ=255)
              tCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                   >  5:214129/1‑149 (MQ=255)
                    cccTGAGCGGGATCGCCCCCTGGCTCAAGCGCAGCCAGGCACCAGGTATCCGTCTTTTCACCATTGATGTGCCTTCGAAGTACAGCCTCAACACCACCACCTCGGACAACGCCGCGGCCGGCCGCG‑GGTTGGCCGAACAGCTGGTgcgc                                                                                                                             <  6:247816/149‑1 (MQ=255)
                                 cGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCggcggc                                                                                                                 >  8:194525/1‑148 (MQ=255)
                                    ccccTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACG‑CGCGGCCGGCCGCGACGTTgtc                                                                                                                                             >  3:90169/1‑115 (MQ=38)
                                    ccccTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACG‑CGCGGCCGGCCGCGACGTTgtc                                                                                                                                             <  4:90169/117‑3 (MQ=38)
                                            tCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATc                                                                                                     >  3:14534/1‑149 (MQ=255)
                                                 cgcATCCAGGCGGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgt                                                                                                <  4:50070/149‑1 (MQ=255)
                                                       cAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGTCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCaa                                                                                          >  4:209653/1‑149 (MQ=255)
                                                       cAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCaa                                                                                          <  3:38474/149‑1 (MQ=255)
                                                        aGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACcggcgg                                                                                                                  >  6:222259/1‑124 (MQ=255)
                                                        aGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACcggcgg                                                                                                                  <  5:222259/124‑1 (MQ=255)
                                                                ggTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCaa                                                                                                               >  5:144684/1‑119 (MQ=255)
                                                                ggTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCaa                                                                                                               <  6:144684/119‑1 (MQ=255)
                                                                     cccGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACgggg                                                                            >  1:320319/1‑149 (MQ=255)
                                                                                 cATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGAGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgtg                                                                >  5:148451/1‑149 (MQ=255)
                                                                                  aTTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGc                                                               >  5:273039/1‑149 (MQ=255)
                                                                                      aggtgCGATCGCAGTACAGCCTCAACAACACCACCTCGGACTACTTCGCGGCCGGCCGCG‑CGTTGTCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCTCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATc                                                           <  6:107432/147‑1 (MQ=255)
                                                                                         tgCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGc                                                        >  2:297466/1‑149 (MQ=255)
                                                                                           ccATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTa                                                      <  6:273039/149‑1 (MQ=255)
                                                                                                gCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTacgac                                                  <  7:166188/148‑1 (MQ=255)
                                                                                                   gTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGt                                              <  7:194525/149‑1 (MQ=255)
                                                                                                      cAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTga                                           <  1:350269/149‑1 (MQ=255)
                                                                                                         ccTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaag                                         >  5:159477/1‑148 (MQ=255)
                                                                                                           tCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGGCTGTGCGATCCGCTACGACGAGTTGaagaag                                      >  7:258943/1‑149 (MQ=255)
                                                                                                            caacaaCACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAgg                                     >  8:284296/1‑149 (MQ=255)
                                                                                                                 acacCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTg                                >  8:314394/1‑149 (MQ=255)
                                                                                                                    ccaccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGcc                             >  7:252713/1‑149 (MQ=255)
                                                                                                                     caccTCGGACAACGTCGCGGCCGGTCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCg                            <  3:209653/149‑1 (MQ=255)
                                                                                                                     caccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGcc                             >  8:268243/1‑148 (MQ=255)
                                                                                                                        cTCGGACAACGTCTCGGCCTGCCGCG‑CGTTGGCAGAACAGCTGTTGCGCTACACCGGCGGCAAGGGCAGCATCCTGTTGTTCAACGGCTTCTACTGGGTGACGGTCTGTTCGATCAGCTACGACGAGTTGAAGAAGGTACTGGCCGat                          >  4:223799/1‑148 (MQ=255)
                                                                                                                             aCAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                                 <  2:301900/104‑1 (MQ=255)
                                                                                                                             aCAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                                 >  1:301900/1‑104 (MQ=255)
                                                                                                                               aaCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTAc                                  >  4:362848/1‑133 (MQ=255)
                                                                                                                               aaCGGCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTAc                                  <  3:362848/133‑1 (MQ=255)
                                                                                                                                     gcgGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCa            <  1:297466/149‑1 (MQ=255)
                                                                                                                                          cggcggCG‑CGTTGGCCGAACAGGTGGTGCGCTACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGATACGACGAGTTGGAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCg       <  2:320319/149‑1 (MQ=255)
                                                                                                                                            gcctCG‑AGTTGGCAGAACAGCTTGTGCGCGACACCGGCGGCAAGGGACGCATCCTGGTGTTCAACGTCTTCTACGGGGTGCCTGTCTGTGCTATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTc     <  3:223799/149‑1 (MQ=255)
                                                                                                                                            gccgCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTc     <  6:214129/149‑1 (MQ=255)
                                                                                                                                               gcg‑agTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAATGGCCTCATCTTTCTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCTCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAg  <  1:304609/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAG  >  NC_002947/3141362‑3141653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑