Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	307,865:1	+C	100%	intergenic (+880/‑989)	hslO → / → PP_0254	molecular chaperone HslO/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	307,865	1	.	C	100.0%	218.9 / NA	63	intergenic (+880/‑989)	hslO/PP_0254	molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (30/33);  total (30/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCA  >  NC_002947/307719‑308003                                                                                                                                                     |                                                                                                                                            aGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAG‑cc                                                                                                                                           >  3:19172/1‑148 (MQ=255)    cATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAG‑ccgtt                                                                                                                                        <  7:217970/149‑5 (MQ=255)    cATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAG‑ccgt                                                                                                                                         >  3:89890/1‑145 (MQ=255)     aTGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAG‑ccgtta                                                                                                                                       <  5:96890/149‑6 (MQ=255)             ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGAt                                                                                                                                  <  5:129026/147‑1 (MQ=255)              gTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTCTTCGGCTGACGAAAGCCGTTACCTGATCg                                                                                                                                <  1:217704/149‑1 (MQ=255)               tGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGt                                                                                                                              <  4:331319/149‑1 (MQ=255)                gCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGt                                                                                                                              >  2:333195/1‑148 (MQ=255)                       ttCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTgacgac                                                                                                                        >  5:176874/1‑147 (MQ=255)                         ccTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGAcgacga                                                                                                                      <  1:159632/147‑1 (MQ=255)                          ctgctgCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGc                                                                                                                    <  5:160869/148‑1 (MQ=255)                             ctgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCAc                                                                                                                  <  1:62569/147‑1 (MQ=255)                                 cggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgg                                                                                                            <  7:142276/149‑1 (MQ=255)                                   gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                          <  7:157165/149‑1 (MQ=255)                                    ctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGc                                                                                                         <  4:89890/149‑1 (MQ=255)                                      gccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCg                                                                                                        >  6:64664/1‑148 (MQ=255)                                        cgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTgacgac                                                                                                                        >  4:75165/1‑130 (MQ=255)                                        cgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTgacgac                                                                                                                        <  3:75165/130‑1 (MQ=255)                                        cgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg                                                                                                     <  5:31249/149‑1 (MQ=255)                                          aTGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGGCGAGCACGGTTGGGGCGAAGG‑c                                                                                                  <  4:362264/149‑1 (MQ=255)                                               cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGtt                                                                                               >  4:47247/1‑147 (MQ=255)                                               cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGtt                                                                                               <  3:47247/147‑1 (MQ=255)                                               cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGAGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTg                                                                                              >  8:277161/1‑148 (MQ=255)                                                 gCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCt                                                                                           >  7:12800/1‑149 (MQ=255)                                                  ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCtt                                                                                          >  7:18802/1‑149 (MQ=255)                                                  ccTATGCACTGCGCGGCCAACACCGGCGAAGAAGGCGATGTGACGTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTc                                                                                            <  8:169598/147‑1 (MQ=255)                                                   cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGtt                                                                                               <  3:302179/143‑1 (MQ=255)                                                   cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGtt                                                                                               >  4:302179/1‑143 (MQ=255)                                                   cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCtt                                                                                          >  7:38468/1‑148 (MQ=255)                                                    tATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTc                                                                                         >  4:63437/1‑148 (MQ=255)                                                      tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCaa                                                                                       <  1:178991/148‑1 (MQ=255)                                                      tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCaa                                                                                       >  1:365147/1‑148 (MQ=255)                                                         aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTCCCCT‑TTCGGCGGCCGAAAGCCGTTACCTGATCGGTGACGCCGAGCACGGTTGGGGCGACGG‑CGTTGTCTTCAACATg                                                                                   >  6:199980/1‑149 (MQ=255)                                                         aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATg                                                                                   <  7:92776/149‑1 (MQ=255)                                                            gcgcGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATgg                                                                                  >  2:82803/1‑147 (MQ=255)                                                              gcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAgg                                                                              <  3:135777/149‑1 (MQ=255)                                                                   cAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGgct                                                                         >  6:332662/1‑149 (MQ=255)                                                                    aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGgctg                                                                        >  7:170152/1‑149 (MQ=255)                                                                        tCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg                                                                                                     <  4:62405/117‑1 (MQ=255)                                                                        tCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg                                                                                                     >  3:62405/1‑117 (MQ=255)                                                                          ggCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                  <  2:103591/149‑1 (MQ=255)                                                                             gaagaaGGCGATGTGACCTTGTTCTTCGTCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                               <  7:277161/149‑1 (MQ=255)                                                                             gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                               <  8:25973/149‑1 (MQ=255)                                                                             gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                               <  5:64664/149‑1 (MQ=255)                                                                                    gCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATc                                                        <  8:38468/149‑1 (MQ=255)                                                                                       aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                     <  4:19172/149‑1 (MQ=255)                                                                                        tgtgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                     <  1:82803/149‑1 (MQ=255)                                                                                           gACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGt                                                 >  2:269131/1‑149 (MQ=255)                                                                                              cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                               >  8:133662/1‑148 (MQ=255)                                                                                              cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                               >  3:175632/1‑148 (MQ=255)                                                                                                 gTTCTTCGTCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGACCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCga                                             <  6:85257/147‑1 (MQ=255)                                                                                                  ttcttcGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgaga                                           <  6:176874/148‑1 (MQ=255)                                                                                                          ccTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGc                                  >  5:194406/1‑149 (MQ=255)                                                                                                           cTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAgac                                 >  7:150503/1‑147 (MQ=255)                                                                                                            tGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                >  1:59271/1‑149 (MQ=255)                                                                                                             gTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                     >  1:8945/1‑127 (MQ=255)                                                                                                             gTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                     <  2:8945/127‑1 (MQ=255)                                                                                                              tCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                <  1:333195/147‑1 (MQ=255)                                                                                                                       ggCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGa                      >  6:220893/1‑148 (MQ=255)                                                                                                                        gCAAGACTACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGa                      >  1:150713/1‑147 (MQ=255)                                                                                                                               tACCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc              >  4:108056/1‑148 (MQ=255)                                                                                                                                aCCCT‑TTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa            <  2:365147/149‑1 (MQ=255)                                                                                                                                    t‑ttCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCAc        >  5:119992/1‑149 (MQ=255)                                                                                                                                         ggCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGgcg    >  6:160008/1‑149 (MQ=255)                                                                                                                                           cTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCa  <  8:12800/149‑1 (MQ=255)                                                                                                                                            tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCAt                           <  2:224840/123‑1 (MQ=255)                                                                                                                                            tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCAt                           >  1:224840/1‑123 (MQ=255)                                                                                                                                                     |                                                                                                                                            AGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCT‑TTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGG‑CGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCA  >  NC_002947/307719‑308003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑