Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,133,977	T→A	6.6%	A351A (GCA→GCT) ‡	PP_3638 ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,133,977	0	T	A	6.6%	174.2 / 2.7	61	A351A (GCA→GCT) ‡	PP_3638	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (26/30);  new base A (2/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

CCCACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCG  >  NC_002947/4133829‑4134125                                                                                                                                                      |                                                                                                                                                     cccACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACgcc‑t                                                                                                                                                      >  7:218964/1‑149 (MQ=255)   cacaCCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACTGGCCTCCAGCAACACCGCGCTCATGCAACgcc‑tgc                                                                                                                                                    <  8:66067/149‑1 (MQ=255)      accacTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGcc‑tgcctg                                                                                                                                                 >  2:343432/1‑149 (MQ=255)        cacTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGc                                                                                                                                               >  7:177307/1‑149 (MQ=255)         acTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCg                                                                                                                                              <  3:102168/149‑1 (MQ=255)          cTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGt                                                                                                                                             <  2:157373/149‑1 (MQ=255)             gggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTg                                                                                                                                           <  6:176125/148‑1 (MQ=255)              ggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCa                                                                                                                                         <  8:177307/149‑1 (MQ=255)                  aaGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTc                                                                                                                                     >  1:317000/1‑149 (MQ=255)                   aGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCg                                                                                                                                    <  2:317000/149‑1 (MQ=255)                      aTGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGgcg                                                                                                                                 >  6:290290/1‑149 (MQ=255)                            aCGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAAt                                                                                                                           >  3:327719/1‑149 (MQ=255)                                ccTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCa                                                                                                                       <  4:327719/149‑1 (MQ=255)                                 cTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑AGGCAGGCGTTGCat                                                                                                                                        <  1:80065/131‑2 (MQ=255)                                 cTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑AGGCAGGCGTTGCat                                                                                                                                        >  2:80065/1‑130 (MQ=255)                                    aCCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCAcc                                                                                                                     <  5:290290/147‑1 (MQ=255)                                          tGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTggg                                                                                                             >  1:367515/1‑149 (MQ=255)                                           gCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑AGGCAGGCGTTGCat                                                                                                                                        <  3:252146/121‑2 (MQ=255)                                           gCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑AGGCAGGCGTTGCat                                                                                                                                        >  4:252146/1‑120 (MQ=255)                                             cAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGt                                                                                                          >  7:254838/1‑149 (MQ=255)                                               gCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCTAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGa                                                                                                        <  1:198671/149‑1 (MQ=255)                                               gCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGa                                                                                                        <  4:75413/149‑1 (MQ=255)                                                             tGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGAc                                                                                                       <  1:140456/136‑1 (MQ=255)                                                             tGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGAc                                                                                                       >  2:140456/1‑136 (MQ=255)                                                               cgcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAg                                                                                          <  8:254838/147‑1 (MQ=255)                                                                gcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGcc                                                                                       >  4:83882/1‑149 (MQ=255)                                                                  cAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGa                                                                                     >  1:175261/1‑149 (MQ=255)                                                                   aGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGAc                                                                                    <  3:324271/149‑1 (MQ=255)                                                                             gcCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTggg                                                                                                             <  7:271447/114‑1 (MQ=255)                                                                             gcCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTggg                                                                                                             >  8:271447/1‑114 (MQ=255)                                                                             gcCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATc                                                                          <  4:217432/149‑1 (MQ=255)                                                                                aaCCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                       >  1:13809/1‑149 (MQ=255)                                                                                aaCCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                       >  1:199849/1‑149 (MQ=255)                                                                                aaCCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                       >  6:378668/1‑149 (MQ=255)                                                                                 accaccCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcg                                                                      <  3:83882/149‑1 (MQ=255)                                                                                  ccaccCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcgg                                                                     <  1:148475/149‑1 (MQ=255)                                                                                     cccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGc                                                                   >  4:136774/1‑148 (MQ=255)                                                                                      ccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCa                                                                  >  4:136650/1‑148 (MQ=255)                                                                                      ccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCa                                                                  >  5:100219/1‑148 (MQ=255)                                                                                       cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                >  7:102014/1‑149 (MQ=255)                                                                                       cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                <  1:343432/149‑1 (MQ=255)                                                                                          aCGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAAccc                                                             >  7:111469/1‑149 (MQ=255)                                                                                           cgcgGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                <  5:45873/145‑1 (MQ=255)                                                                                           cgcgGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                >  6:45873/1‑145 (MQ=255)                                                                                            gcgGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCtt                                                           >  7:60833/1‑149 (MQ=255)                                                                                              gggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCg                                                         <  8:102014/149‑1 (MQ=255)                                                                                               ggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGc                                                        >  7:95589/1‑149 (MQ=255)                                                                                                       aaGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                <  1:152836/133‑1 (MQ=255)                                                                                                       aaGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                >  2:152836/1‑133 (MQ=255)                                                                                                        aGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCg                                                >  5:292773/1‑148 (MQ=255)                                                                                                           ggATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCaga                                            <  2:175261/149‑1 (MQ=255)                                                                                                              tGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGc                                                        <  5:71279/134‑1 (MQ=255)                                                                                                              tGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGc                                                        >  6:71279/1‑134 (MQ=255)                                                                                                                    gggCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCt                                   <  8:111469/149‑1 (MQ=255)                                                                                                                       ccTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgc                                <  4:129530/149‑1 (MQ=255)                                                                                                                        cTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgcc                               <  2:13809/149‑1 (MQ=255)                                                                                                                           cAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgccgcc                            <  8:218964/149‑1 (MQ=255)                                                                                                                             gCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCAc                          <  2:307361/149‑1 (MQ=255)                                                                                                                                  acCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGTCTCCCGCCATCGGCggg                                                                    <  3:241452/102‑1 (MQ=255)                                                                                                                                  acCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCggg                                                                    >  4:241452/1‑102 (MQ=255)                                                                                                                                    cgcgCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTg                   <  6:100219/149‑1 (MQ=255)                                                                                                                                     ccgcTCATGC‑ACGCCGGGCCGGGGGTTGCTGTGCGGCGCGCAATTCCCCCCGGCGGGGCGTGACGACCGGCACTACGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTGTTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGc                  <  2:367515/148‑1 (MQ=255)                                                                                                                                               aaCGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt        <  7:13409/149‑1 (MQ=255)                                                                                                                                               aaCGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt        >  6:65303/1‑149 (MQ=255)                                                                                                                                                  gcc‑tgcctGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTa     >  4:266625/1‑149 (MQ=255)                                                                                                                                                   cc‑tgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTAc    <  3:136774/149‑1 (MQ=255)                                                                                                                                                      tgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACcg  >  5:241114/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CCCACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCG  >  NC_002947/4133829‑4134125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑