Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,133,979	C→G	6.6%	A351P (GCA→CCA) ‡	PP_3638 ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,133,979	0	C	G	6.6%	195.6 / 4.1	61	A351P (GCA→CCA) ‡	PP_3638	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (26/31);  new base G (2/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

CACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCGCG  >  NC_002947/4133831‑4134127                                                                                                                                                      |                                                                                                                                                     cacaCCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACTGGCCTCCAGCAACACCGCGCTCATGCAACgcc‑tgc                                                                                                                                                      <  8:66067/149‑1 (MQ=255)    accacTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGcc‑tgcctg                                                                                                                                                   >  2:343432/1‑149 (MQ=255)      cacTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGc                                                                                                                                                 >  7:177307/1‑149 (MQ=255)       acTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCg                                                                                                                                                <  3:102168/149‑1 (MQ=255)        cTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGt                                                                                                                                               <  2:157373/149‑1 (MQ=255)           gggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTg                                                                                                                                             <  6:176125/148‑1 (MQ=255)            ggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCa                                                                                                                                           <  8:177307/149‑1 (MQ=255)                aaGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTc                                                                                                                                       >  1:317000/1‑149 (MQ=255)                 aGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCg                                                                                                                                      <  2:317000/149‑1 (MQ=255)                    aTGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGgcg                                                                                                                                   >  6:290290/1‑149 (MQ=255)                          aCGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAAt                                                                                                                             >  3:327719/1‑149 (MQ=255)                              ccTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCa                                                                                                                         <  4:327719/149‑1 (MQ=255)                               cTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑AGGCAGGCGTTGCat                                                                                                                                          <  1:80065/131‑2 (MQ=255)                               cTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑AGGCAGGCGTTGCat                                                                                                                                          >  2:80065/1‑130 (MQ=255)                                  aCCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCAcc                                                                                                                       <  5:290290/147‑1 (MQ=255)                                        tGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTggg                                                                                                               >  1:367515/1‑149 (MQ=255)                                         gCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑AGGCAGGCGTTGCat                                                                                                                                          <  3:252146/121‑2 (MQ=255)                                         gCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑AGGCAGGCGTTGCat                                                                                                                                          >  4:252146/1‑120 (MQ=255)                                           cAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGt                                                                                                            >  7:254838/1‑149 (MQ=255)                                             gCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCTAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGa                                                                                                          <  1:198671/149‑1 (MQ=255)                                             gCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGa                                                                                                          <  4:75413/149‑1 (MQ=255)                                                           tGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGAc                                                                                                         <  1:140456/136‑1 (MQ=255)                                                           tGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGAc                                                                                                         >  2:140456/1‑136 (MQ=255)                                                             cgcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAg                                                                                            <  8:254838/147‑1 (MQ=255)                                                              gcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGcc                                                                                         >  4:83882/1‑149 (MQ=255)                                                                cAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGa                                                                                       >  1:175261/1‑149 (MQ=255)                                                                 aGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGAc                                                                                      <  3:324271/149‑1 (MQ=255)                                                                           gcCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTggg                                                                                                               <  7:271447/114‑1 (MQ=255)                                                                           gcCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTggg                                                                                                               >  8:271447/1‑114 (MQ=255)                                                                           gcCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATc                                                                            <  4:217432/149‑1 (MQ=255)                                                                              aaCCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                         >  1:199849/1‑149 (MQ=255)                                                                              aaCCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                         >  6:378668/1‑149 (MQ=255)                                                                              aaCCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                         >  1:13809/1‑149 (MQ=255)                                                                               accaccCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcg                                                                        <  3:83882/149‑1 (MQ=255)                                                                                ccaccCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcgg                                                                       <  1:148475/149‑1 (MQ=255)                                                                                   cccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGc                                                                     >  4:136774/1‑148 (MQ=255)                                                                                    ccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCa                                                                    >  5:100219/1‑148 (MQ=255)                                                                                    ccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCa                                                                    >  4:136650/1‑148 (MQ=255)                                                                                     cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                  <  1:343432/149‑1 (MQ=255)                                                                                     cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                  >  7:102014/1‑149 (MQ=255)                                                                                        aCGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAAccc                                                               >  7:111469/1‑149 (MQ=255)                                                                                         cgcgGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                  >  6:45873/1‑145 (MQ=255)                                                                                         cgcgGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                  <  5:45873/145‑1 (MQ=255)                                                                                          gcgGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCtt                                                             >  7:60833/1‑149 (MQ=255)                                                                                            gggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCg                                                           <  8:102014/149‑1 (MQ=255)                                                                                             ggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGc                                                          >  7:95589/1‑149 (MQ=255)                                                                                                     aaGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                  >  2:152836/1‑133 (MQ=255)                                                                                                     aaGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                  <  1:152836/133‑1 (MQ=255)                                                                                                      aGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCg                                                  >  5:292773/1‑148 (MQ=255)                                                                                                         ggATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCaga                                              <  2:175261/149‑1 (MQ=255)                                                                                                            tGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGc                                                          >  6:71279/1‑134 (MQ=255)                                                                                                            tGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGc                                                          <  5:71279/134‑1 (MQ=255)                                                                                                                  gggCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCt                                     <  8:111469/149‑1 (MQ=255)                                                                                                                     ccTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgc                                  <  4:129530/149‑1 (MQ=255)                                                                                                                      cTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgcc                                 <  2:13809/149‑1 (MQ=255)                                                                                                                         cAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgccgcc                              <  8:218964/149‑1 (MQ=255)                                                                                                                           gCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCAc                            <  2:307361/149‑1 (MQ=255)                                                                                                                                acCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGTCTCCCGCCATCGGCggg                                                                      <  3:241452/102‑1 (MQ=255)                                                                                                                                acCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCggg                                                                      >  4:241452/1‑102 (MQ=255)                                                                                                                                  cgcgCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTg                     <  6:100219/149‑1 (MQ=255)                                                                                                                                   ccgcTCATGC‑ACGCCGGGCCGGGGGTTGCTGTGCGGCGCGCAATTCCCCCCGGCGGGGCGTGACGACCGGCACTACGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTGTTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGc                    <  2:367515/148‑1 (MQ=255)                                                                                                                                             aaCGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt          <  7:13409/149‑1 (MQ=255)                                                                                                                                             aaCGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt          >  6:65303/1‑149 (MQ=255)                                                                                                                                                gcc‑tgcctGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTa       >  4:266625/1‑149 (MQ=255)                                                                                                                                                 cc‑tgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTAc      <  3:136774/149‑1 (MQ=255)                                                                                                                                                    tgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACcg    >  5:241114/1‑149 (MQ=255)                                                                                                                                                      cctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACcgcg  <  6:241114/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCC‑TGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCGCG  >  NC_002947/4133831‑4134127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑