Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,143,205	G→C	7.0%	S446S (TCG→TCC)	PP_3646 →	aldehyde dehydrogenase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,143,205	0	G	C	7.0%	212.5 / 2.5	67	S446S (TCG→TCC)	PP_3646	aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (31/31);  new base C (2/3);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAGAAGAAGCCCTGGCCATGGCCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCGACCCGTTCGTGATGCGC  >  NC_002947/4143061‑4143346                                                                                                                                                  |                                                                                                                                               cGAAGAAGAAGCCCTGGCCATGGCCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATGATCTGGGTCAACACCTACCGTGCCGTGTCGGCCa                                                                                                                                            >  4:16711/1‑149 (MQ=255) cGAAGAAGAAGCCCTGGCCATGGCCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCa                                                                                                                                            <  4:313614/149‑1 (MQ=255)  gaagaagaaGCCCTGGCCATGGCCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCAt                                                                                                                                           >  4:42298/1‑149 (MQ=255)    agaagaagCCCTGGCCATGGCCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATgg                                                                                                                                         <  3:34117/149‑1 (MQ=255)      aagaagCCCTGGCCATGGCCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCa                                                                                                                                       >  2:123160/1‑149 (MQ=255)        gaagCCCTGGCCATGACCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCATGGCCGACACGGCAcg                                                                                                                                     <  1:275926/149‑2 (MQ=255)           gCCCTGGCCATGTCCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑t                                                                                                                                 <  8:245137/149‑1 (MQ=255)              cTGGCCATGGCCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAgg                                                                                                                              >  5:230743/1‑149 (MQ=255)                     tggccaACGACTCTCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGTCCCGCGACGTGCGTTCGGGGATCATCTTGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TATGTGGTTTc                                                                                                                       <  6:156780/149‑1 (MQ=255)                       gccaACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGTGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCaa                                                                                                                     <  2:110891/149‑1 (MQ=255)                        ccaACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTc                                                                                                                       >  3:254489/1‑146 (MQ=255)                         caACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGa                                                                                                                   <  1:123160/149‑1 (MQ=255)                               cTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCg                                                                                                             <  3:179486/149‑1 (MQ=255)                                tCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCgg                                                                                                            >  1:192635/1‑149 (MQ=255)                                  gCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCAGCGA‑TAGGTGGTTTCAAGAACAGCGGc                                                                                                           >  4:229684/1‑148 (MQ=255)                                      ttCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCgg                                                                                                            >  5:220303/1‑143 (MQ=255)                                      ttCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCgg                                                                                                            <  6:220303/143‑1 (MQ=255)                                          gCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGt                                                                                                  <  2:192635/149‑1 (MQ=255)                                           ccTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGctcgc                                                                                                 >  1:133923/1‑145 (MQ=255)                                           ccTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGt                                                                                                  <  2:133923/148‑1 (MQ=255)                                            cTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTga                                                                                                <  3:42298/149‑1 (MQ=255)                                                 tGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCCCCGA‑TAGGTGGTTTCAAGAACAGCg                                                                                                             <  5:80490/131‑1 (MQ=255)                                                 tGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCg                                                                                                             <  5:85824/131‑1 (MQ=255)                                                 tGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCg                                                                                                             >  6:80490/1‑131 (MQ=255)                                                 tGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCg                                                                                                             >  6:85824/1‑131 (MQ=255)                                                  gCCGGCATCTGGACGCGCGATCTGGGCCGTGGCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCg                                                                                           >  1:39253/1‑149 (MQ=255)                                                    cGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCa                                                                                        <  5:368419/149‑1 (MQ=255)                                                     ggCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCATGAACAGCGGCTACGGCCGTGAGAGCGGCAt                                                                                       <  1:30101/149‑1 (MQ=255)                                                               cgcgcgATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTg                                                                             <  6:194386/149‑1 (MQ=255)                                                                  gcgATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGCGCGGCAGCGATGAGGTGCTg                                                                          >  6:5438/1‑149 (MQ=255)                                                                  gcgATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTg                                                                          >  2:359000/1‑149 (MQ=255)                                                                   cgATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTg                                                                          >  7:253178/1‑148 (MQ=255)                                                                   cgATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTg                                                                          <  8:253178/148‑1 (MQ=255)                                                                          ggCCGT‑GCCCACCGCATGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCt                                                                      <  5:5438/145‑1 (MQ=255)                                                                            ccGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCg                                                                >  8:24411/1‑149 (MQ=255)                                                                            ccGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCg                                                                >  8:30032/1‑149 (MQ=255)                                                                             cGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGa                                                                                    >  1:51657/1‑128 (MQ=255)                                                                             cGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGa                                                                                    <  2:51657/128‑1 (MQ=255)                                                                             cGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGa                                                               <  3:229684/149‑1 (MQ=255)                                                                                             gCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCgtgt                                                 <  6:230743/148‑1 (MQ=255)                                                                                             gCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCgtg                                                  <  1:359000/147‑1 (MQ=255)                                                                                                       tGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGc                                                             <  7:270492/126‑1 (MQ=255)                                                                                                       tGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGc                                                             >  8:270492/1‑126 (MQ=255)                                                                                                         cGTTCGGGGATGATCTTGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTc                                    <  3:16711/149‑1 (MQ=255)                                                                                                         cGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTc                                    <  5:323511/149‑1 (MQ=255)                                                                                                           ttCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCa                                  >  7:181101/1‑149 (MQ=255)                                                                                                           ttCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCa                                  >  4:321019/1‑149 (MQ=255)                                                                                                                  atcatcTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGt                                                                                                  <  7:53328/78‑1 (MQ=255)                                                                                                                  atcatcTGGGTAAACACCTACCGTGCCGTGTCCGCAATGGCACCGA‑TAGGTGCATTCAAGAACATCGGCTACGGccaa                                                                                                  >  8:53328/1‑76 (MQ=255)                                                                                                                     atcTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGAt                        >  4:324433/1‑149 (MQ=255)                                                                                                                     atcTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGAt                        >  7:32776/1‑149 (MQ=255)                                                                                                                       cTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTc                                                                                 >  4:290951/1‑90 (MQ=255)                                                                                                                       cTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTc                                                                                 <  3:290951/90‑1 (MQ=255)                                                                                                                          ggTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCg                   >  3:320937/1‑149 (MQ=255)                                                                                                                                  ccTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCGACCCGTTc           <  4:254489/149‑1 (MQ=255)                                                                                                                                   cTACCGTGCCGTGTCGGCCATGGCACCGAGTAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGcc                     >  1:218298/1‑139 (MQ=255)                                                                                                                                   cTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGcc                     <  2:218298/138‑1 (MQ=255)                                                                                                                                   cTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCGACCCGTTCg          >  4:354284/1‑149 (MQ=255)                                                                                                                                   cTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGATACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCGACCCGTTCg          >  1:280914/1‑149 (MQ=255)                                                                                                                                      ccgtgccgtgTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTTGATCAAc                                         <  5:108346/115‑1 (MQ=255)                                                                                                                                      ccgtgccgtgTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAAc                                         >  6:108346/1‑115 (MQ=255)                                                                                                                                      ccgtgccgtgTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCGACCCGTTCGTGa       <  2:39253/149‑1 (MQ=255)                                                                                                                                       cgtgccgtgTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCGACCCGTTCGTGAt      >  2:134600/1‑149 (MQ=255)                                                                                                                                        gtgccatgGCCGACACGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGcc                     <  3:346212/133‑1 (MQ=255)                                                                                                                                        gtgccatgGCCGACACGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGcc                     >  4:346212/1‑133 (MQ=255)                                                                                                                                           ccgtgTCCGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCGACCCGTTCGTGATgcg   <  3:321019/148‑1 (MQ=255)                                                                                                                                            cgtgTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCGACCCGTTCGTGATgcgc  >  5:192606/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                               CGAAGAAGAAGCCCTGGCCATGGCCAACGACTCGCAATTCGGCCTGGCTGCCGGCATCTGGACGCGCGATCTGGGCCGT‑GCCCACCGCCTGGCCCGCGACGTGCGTTCGGGGATCATCTGGGTCAACACCTACCGTGCCGTGTCGGCCATGGCACCGA‑TAGGTGGTTTCAAGAACAGCGGCTACGGCCGTGAGAGCGGCATCGATTCGGTGCTGGCCTATACCGAGCTGAAGACCGTGTGGATCAACTTGTCCACCGCGCCGATGCCCGACCCGTTCGTGATGCGC  >  NC_002947/4143061‑4143346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑