Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,149,783	G→C	9.6%	V79L (GTG→CTG)	PP_3654 →	leucine‑responsive regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,149,783	0	G	C	9.6%	205.2 / 12.7	73	V79L (GTG→CTG)	PP_3654	leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (35/31);  new base C (5/2);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.45e-01

GCCTGTCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGACGAGCACCTGACCAGTATTCC  >  NC_002947/4149637‑4149931                                                                                                                                                   |                                                                                                                                                         gCCGGTCACCCTGCCATCGGCGCTTTAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGTGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTg                                                                                                                                                        <  4:301308/149‑1 (MQ=255)  ccTGTCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTgg                                                                                                                                                       >  8:86186/1‑149 (MQ=255)    tGTCACCCTGACATCGGCGCTTGAAGGCACTGGAAGAGTCCGCAGCCATTCTGGACTATCACCCACGGCTCGCACCGAGCGCCCTTGCCCTCAACTTTGCCGCGCTGGTAATCGTGACGATACGCGAGGTAACCCGCCAGCCGGtctcg                                                                                                                                                     >  2:96297/1‑145 (MQ=255)     gTCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCgg                                                                                                                                                    >  7:350185/1‑149 (MQ=255)         cccTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACtt                                                                                                                                                >  1:38530/1‑149 (MQ=255)         cccTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACtt                                                                                                                                                >  2:69237/1‑149 (MQ=255)          ccTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACttt                                                                                                                                               <  6:192902/149‑1 (MQ=255)          ccTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACttt                                                                                                                                               >  5:15844/1‑149 (MQ=255)          ccTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACttt                                                                                                                                               <  6:380109/149‑1 (MQ=255)          ccTGACATCGGAGCTTGAAGGCACTGGAAGAGTCCGGAGCCATACTGGGCTATCACGCAAGGCTGGCACCGAGCGCCCTAGGGCTGAACTTTGACGCGCTGCTATTCGTGACGCTACGCGAGGTAACCCGCCAGCAGGTGGCGGACttt                                                                                                                                               >  5:338069/1‑149 (MQ=255)            tGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTg                                                                                                                                              >  2:107431/1‑148 (MQ=255)                aTCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACg                                                                                                                                        >  8:238518/1‑149 (MQ=255)                aTCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACg                                                                                                                                        >  3:52049/1‑149 (MQ=255)                              gCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGgcg                                                                                                                               >  1:353739/1‑144 (MQ=255)                                aCTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATcc                                                                                                                       >  7:220954/1‑149 (MQ=255)                                aCTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATcc                                                                                                                       >  2:86503/1‑149 (MQ=255)                                     aaGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGAC‑TTGAA‑ACGGCACTGGCGCA‑GATCCCg                                                                                                                     >  7:273420/1‑145 (MQ=255)                                      agagTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGa                                                                                                                 <  5:3976/149‑1 (MQ=255)                                       gagTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGAt                                                                                                                >  5:126362/1‑149 (MQ=255)                                          tCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGt                                                                                                             <  2:38530/149‑1 (MQ=255)                                             ggAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCg                                                                                                           >  6:242846/1‑148 (MQ=255)                                             ggAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGCGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCg                                                                                                           >  8:80811/1‑148 (MQ=255)                                             ggAGCCATGCTGGGCTTTCTCGCACGGGTGGCACCGAGCGCCCTTGGGATGAACTTTGCCGCGCTGTTATTCTTGACGCTACGCGAGGTAACCCTCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGa                                                                                                          <  1:96297/149‑1 (MQ=255)                                              gAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCg                                                                                                           >  3:259159/1‑147 (MQ=255)                                               aGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GAt                                                                                                                         >  3:118580/1‑132 (MQ=255)                                               aGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GAt                                                                                                                         <  4:118580/132‑1 (MQ=255)                                                    ttCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCTTGACGCTACGCGAGGTAACGCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAg                                                                                                   <  7:238518/149‑1 (MQ=255)                                                        gggCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGc                                                                                               >  4:85277/1‑149 (MQ=255)                                                        gggCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGc                                                                                               >  6:266896/1‑149 (MQ=255)                                                        gggCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGc                                                                                               >  8:83159/1‑149 (MQ=255)                                                              tCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGTGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTc                                                                                          <  4:307770/148‑1 (MQ=255)                                                              tCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAACACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTc                                                                                          <  1:86503/149‑1 (MQ=255)                                                               cacgcacgGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCgg                                                                                        >  8:24307/1‑149 (MQ=255)                                                                acgcacgGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCgg                                                                                        <  8:39535/148‑1 (MQ=255)                                                                 cgcacgGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCTCTGGCGCA‑GATCCCGCTGATTGTCGAAGCGCAGCGGCTGTTCGGCg                                                                                      <  7:83159/149‑1 (MQ=255)                                                                 cgcacgGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCCGCGGCTGTTCGGc                                                                                       >  2:269551/1‑148 (MQ=255)                                                                  gcacgGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑a                                                                                    <  4:259159/149‑1 (MQ=255)                                                                    acgGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑Acc                                                                                  >  8:183174/1‑149 (MQ=255)                                                                        cTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGa                                                                              >  1:149633/1‑149 (MQ=255)                                                                              ccGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACct                                                                        <  4:52049/149‑1 (MQ=255)                                                                                   cgcCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCtgctgc                                                                   <  1:69237/149‑1 (MQ=255)                                                                                          gggCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCAtgtg                                                              <  8:350185/147‑1 (MQ=255)                                                                                          gggCTGAACTTTGCCGCGCTGGTATTCGCGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTgg                                                             <  7:80811/148‑1 (MQ=255)                                                                                                   tttGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGa                                                   >  4:269048/1‑149 (MQ=255)                                                                                                   tttGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGa                                                   >  7:127335/1‑149 (MQ=255)                                                                                                     tGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGa                                                   <  2:11864/147‑1 (MQ=255)                                                                                                     tGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTAACTGCTGCATGTGGTAGCCAAGGAc                                                  <  5:242846/148‑1 (MQ=255)                                                                                                       ccGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCt                                                <  7:183174/148‑1 (MQ=255)                                                                                                        cgcgCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGc                                              <  5:10433/149‑1 (MQ=255)                                                                                                             tGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCg                                        >  7:309437/1‑149 (MQ=255)                                                                                                               gTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCg                                        <  3:85277/147‑1 (MQ=255)                                                                                                                tATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCACGGCgct                                      <  6:126362/149‑3 (MQ=255)                                                                                                                  ttCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCa                                   <  5:177083/149‑1 (MQ=255)                                                                                                                     gTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAg                                  >  4:357251/1‑147 (MQ=255)                                                                                                                      tGAAGCTAGGCGATGTAACCGGCCAGCAGGTGGCGGACTTTGAA‑AGGGAAGTGGCGCA‑GATCCGGCAGTTTGTCGAAGCGCAGCGGCTGTTCGTCG‑ACCCGGATTAGCTGCTGCATGTGGTTCCCAAGGACCTGC‑CGGCGTTTCAGAAg                               <  6:338069/149‑1 (MQ=255)                                                                                                                        aCGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCt                             <  3:269048/149‑1 (MQ=255)                                                                                                                         cGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGACGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTg                            <  6:15844/149‑1 (MQ=255)                                                                                                                             aCGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCAGGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTAt                         >  1:279756/1‑149 (MQ=255)                                                                                                                              cgcgAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGAACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTAt                         <  1:107431/148‑1 (MQ=255)                                                                                                                               gcgAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGAc                      >  4:137548/1‑149 (MQ=255)                                                                                                                               gcgAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGAc                      <  7:24307/149‑1 (MQ=255)                                                                                                                                 gAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTa                                                           <  8:131372/111‑1 (MQ=255)                                                                                                                                 gAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTa                                                           >  7:131372/1‑111 (MQ=255)                                                                                                                                    aaagtccGCCACCGGCTGGCGGAGTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGACGAGCa                 >  5:336044/6‑149 (MQ=255)                                                                                                                                      agtccGCCACCGGCTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GCTCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑CCCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGGTTCAGAAGCTGTAGGACGCGCAcc               >  7:5747/4‑149 (MQ=255)                                                                                                                                      agtccGCCACCGGCTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑CCCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTGTCAGAAGCTGTATGACGAGCAcc               >  7:11106/4‑149 (MQ=255)                                                                                                                                      aaCCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGACGAGCAcc               >  1:329667/1‑149 (MQ=255)                                                                                                                                       gtccGCCACCGGCTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGa                       >  7:304507/3‑140 (MQ=255)                                                                                                                                       gtccGCCACCGGCTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGa                       >  7:299180/3‑140 (MQ=255)                                                                                                                                       gtccGCCACCGGCTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGa                       <  8:299180/138‑1 (MQ=255)                                                                                                                                       gtccGCCACCGGCTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGa                       <  8:304507/138‑1 (MQ=255)                                                                                                                                       aCCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGACGAGCACCt              >  3:121312/1‑149 (MQ=255)                                                                                                                                          cGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGACGAGCACCTGAc           <  7:86186/149‑1 (MQ=255)                                                                                                                                                   gTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGACGAGCACCTGACCAGTATTcc  >  2:126199/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                         GCCTGTCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAA‑ACGGCACTGGCGCA‑GATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCG‑ACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGC‑CGGCGTTTCAGAAGCTGTATGACGAGCACCTGACCAGTATTCC  >  NC_002947/4149637‑4149931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑