Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,198,406 C→T 8.5% L81L (CTG→TTG)  PP_3689 → serine/threonine protein phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,198,4060CT 8.5% 133.9 / 2.7 47L81L (CTG→TTG) PP_3689serine/threonine protein phosphatase
Reads supporting (aligned to +/- strand):  ref base C (21/22);  new base T (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

GCCTGCAGGCTCTCGGCTTTGATCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGAAATCGAATCAGCTCA  >  NC_002947/4198260‑4198551
                                                                                                                                                  |                                                                                                                                                 
gCCTGCAGGCTCTCGGCTTTGATCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCt                                                                                                                                                  <  5:377970/148‑1 (MQ=255)
 ccTGCAGGCTCTCGGCTTTGATCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGTGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGa                                                                                                                                                <  1:77970/149‑1 (MQ=255)
      aGGCTCTCGGCTTTGATCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAAc                                                                                                                                           <  2:371589/149‑1 (MQ=255)
          tctcGGCTTTGATCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGc                                                                                                                                                   >  1:124425/1‑137 (MQ=255)
          tctcGGCTTTGATCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGc                                                                                                                                                   <  2:124425/137‑1 (MQ=255)
                   tGATCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCa                                                                                                                               <  6:90414/148‑1 (MQ=255)
                    gATCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATg                                                                                                                                 >  2:6717/1‑145 (MQ=255)
                      tCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATc                                                                                                                           >  8:96718/1‑149 (MQ=255)
                        aGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATcc                                                                                                                          <  4:273566/148‑1 (MQ=255)
                         ggCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATc                                                                                                                           >  2:253050/1‑146 (MQ=255)
                           ccaccGACAGGTGTATCGCGGGGGGCGAGGTCTGCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGAt                                                                                                                      <  6:268736/145‑1 (MQ=255)
                                 aCAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGc                                                                                                                >  7:249213/1‑149 (MQ=255)
                                   aGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTa                                                                                                              >  3:354169/1‑149 (MQ=255)
                                    ggTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTAt                                                                                                             <  3:6017/149‑1 (MQ=255)
                                     gTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATg                                                                                                            >  8:4591/1‑149 (MQ=255)
                                         aTCGCTGTGGGCGATGGCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCcg                                                                                                         <  2:99213/148‑1 (MQ=255)
                                                    cGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATgg                                                                                             >  7:28561/1‑149 (MQ=255)
                                                     gATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGAt                                                                                                                      >  3:326154/1‑123 (MQ=255)
                                                     gATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGAt                                                                                                                      <  4:326154/123‑1 (MQ=255)
                                                           aTCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTtggtg                                                                                      >  7:134382/1‑149 (MQ=255)
                                                           aTCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTtggt                                                                                       <  1:253050/148‑1 (MQ=255)
                                                           aTCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTtggt                                                                                       <  8:249213/148‑1 (MQ=255)
                                                                      gCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTc                                                                           <  3:298981/149‑1 (MQ=255)
                                                                        cGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCa                                                                         >  5:165244/1‑149 (MQ=255)
                                                                         gggggTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCAt                                                                        <  7:4591/149‑1 (MQ=255)
                                                                                 gggAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATc                                                                 <  4:354169/148‑1 (MQ=255)
                                                                                     ggTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGaa                                                              >  4:297438/1‑147 (MQ=255)
                                                                                      gTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGaaaa                                                            <  3:44201/148‑1 (MQ=255)
                                                                                         cTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGTTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATGCGAATCGAAAAGcgt                                                        >  6:25917/1‑149 (MQ=255)
                                                                                         cTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGTTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGcgt                                                        >  6:374105/1‑149 (MQ=255)
                                                                                            cAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGcgtc                                                       <  8:28561/147‑1 (MQ=255)
                                                                                                  cTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCg                                                >  6:209585/1‑148 (MQ=255)
                                                                                                      atgaGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGaaaaa                                           >  3:51052/1‑149 (MQ=255)
                                                                                                           ccATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTgcg                                      >  5:91363/1‑149 (MQ=255)
                                                                                                             aTGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCa                                    <  7:96718/149‑1 (MQ=255)
                                                                                                             aTGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCCATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTgcg                                      >  1:260897/1‑147 (MQ=255)
                                                                                                                      taGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGa                           >  3:167279/1‑149 (MQ=255)
                                                                                                                        gCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGAt                          <  3:297438/148‑1 (MQ=255)
                                                                                                                          gTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGc                       <  8:134382/149‑1 (MQ=255)
                                                                                                                           tCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGc                       >  1:191688/1‑148 (MQ=255)
                                                                                                                                ggCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTg                  >  1:93857/1‑148 (MQ=255)
                                                                                                                                   aaCCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGaaa               >  3:193426/1‑148 (MQ=255)
                                                                                                                                   aaCCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGAAAt              >  3:367678/1‑149 (MQ=255)
                                                                                                                                      cATGAGCAGATGTTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATGCGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGAAATCGa           <  5:25917/149‑1 (MQ=255)
                                                                                                                                      cATGAGCAGATGTTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGAAATCGa           <  5:374105/149‑1 (MQ=255)
                                                                                                                                          aGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGAAATCGAATc        <  5:209585/148‑1 (MQ=255)
                                                                                                                                           gCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGAAATCGAATCAg      <  2:191688/149‑1 (MQ=255)
                                                                                                                                             aGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGAAATCGAATCAGc     >  3:161694/1‑148 (MQ=255)
                                                                                                                                               aTGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGAAATCGAATCAGCTCa  <  4:161694/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCTGCAGGCTCTCGGCTTTGATCAGGCGATCGACAGGTTGATCGCTGTGGGCGATGTCATCGACCGAGGGCCGGGGGTTCGGGAGGTGCTCCAACTGCTTGATGAGCCATGGTTCTATAGCGTCCAAGGCAACCATGAGCAGATGCTGATCAACGCCTACCATGCAGATCCAGATGTTCGCTATGCCGCGCATGCGATGGTTTGGTGGCCAATGATTCCATACGAATCGAAAAGCGTCGTGATCGAAAAACTGCGCAGCCTTCCGATCGCCATTGAAATCGAATCAGCTCA  >  NC_002947/4198260‑4198551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑