Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 337,809 A→G 17.0% intergenic (‑59/+70) PP_0279 ← / ← PP_0280 hypothetical protein/amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947337,8090AG17.0% 102.5 / 14.3 48intergenic (‑59/+70)PP_0279/PP_0280hypothetical protein/amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (23/16);  new base G (4/4);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01

CCACAGCTGCTGGTGCTCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCAC  >  NC_002947/337667‑337956
                                                                                                                                              |                                                                                                                                                    
ccACAGCTGCTGGTGCTCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAgcgc                                                                                                                                                >  5:85314/1‑149 (MQ=255)
acacaGCTGCTGGTGCTCGATAAGTAACTGGTCGAGGGCCTGGAATCGGGTGCTTAGGTGGGGGATGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGTAACGCCCGCTCACACAGGTAAAgcgc                                                                                                                                                >  3:83286/2‑149 (MQ=255)
 cacaGCTGCTGGTGCTCGATCAGGCACTGGGCGAGGGCCTGGAAGCGTGTGCTTAGGTGGGGGCGGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTGTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAgc                                                                                                                                                  <  4:247296/146‑1 (MQ=39)
   caGCTGCTGGTGCTCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCc‑ac                                                                                                                                            <  8:33736/149‑1 (MQ=255)
    aGCTGCTGGTGCTCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCc‑aca                                                                                                                                           <  6:85314/149‑1 (MQ=255)
    aGCTGCTGGTGCTCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCGCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCc‑aac                                                                                                                                           >  2:68363/1‑147 (MQ=255)
       tgctgGTGCTCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACAtt                                                                                                                                         >  7:259680/1‑148 (MQ=255)
              gCTCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTg                                                                                                                                 >  8:204696/1‑149 (MQ=255)
               cTCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTAAAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTgg                                                                                                                                >  1:283841/1‑149 (MQ=255)
                tCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGgc                                                                                                                               >  5:290657/1‑149 (MQ=255)
                  gATCATGATCTGTTAGAGGGACTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGTGGTGGTTGGGCATGTAAAGGATGTGTGTTGGTTTTGAGTTCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGgcgc                                                                                                                             <  4:83286/149‑1 (MQ=255)
                  gATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGgcgc                                                                                                                             <  6:90229/149‑1 (MQ=255)
                    tCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTa                                                                                                                           <  7:204696/149‑1 (MQ=255)
                       gttccTGATCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTacac                                                                                                                        <  8:161941/145‑1 (MQ=255)
                       ggAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGAGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGGC‑ACATTCAAGGTTGGCGCTacac                                                                                                                        >  1:8633/1‑149 (MQ=255)
                        gAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACAcc                                                                                                                       >  1:338363/1‑149 (MQ=255)
                           cTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCtgt                                                                                                                    <  2:338363/149‑1 (MQ=255)
                           cTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGGGGGGGCTGGGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCtgt                                                                                                                    <  4:213182/149‑1 (MQ=255)
                           atGGTCGAGGGACTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGAGGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCtgt                                                                                                                    <  4:217920/148‑1 (MQ=255)
                            tGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑CCAGTCAAGGTTGGCGCTACACCtgtg                                                                                                                   >  5:93914/1‑149 (MQ=255)
                              gTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTgg                                                                                                                  <  7:192438/148‑1 (MQ=255)
                              gTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCGCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATG‑TGGCGCTATACCtgtg                                                                                                                   <  1:68363/147‑1 (MQ=21)
                                 gAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGc                                                                                                              >  2:13695/1‑149 (MQ=255)
                                   gggCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCgg                                                                                                            <  1:13695/149‑1 (MQ=255)
                                                ggTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGaa                                                                                               >  4:324270/1‑149 (MQ=255)
                                                              ggCTGTGGTGGTGGTGGGGCATGGCAAGGATGGGTGGGGGTTTTGCGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATACCGCC‑ACATTCACGGTTGGCGCTACACCTGGGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGt                                                                                 <  2:283841/149‑1 (MQ=37)
                                                              ggCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATG‑TGGCGCTATACCtgt                                                                                                                    <  5:176621/114‑1 (MQ=18)
                                                              ggCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATG‑TGGCGCTATACCtgt                                                                                                                    >  6:176621/1‑114 (MQ=18)
                                                                  gtgGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGtct                                                                               >  5:141935/1‑147 (MQ=255)
                                                                        gtggtTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGcc                                                                       >  3:141740/1‑149 (MQ=255)
                                                                               ggCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTggcag                                                                <  3:324270/149‑1 (MQ=255)
                                                                                          gATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCCCAGGTATAACGCC‑ACAATGAAGGTTGGGGCGACACCGGTGGGAGCCGGCGAGCCCCCCAAGCACTCACAGACGTATAACCGGCCATGGCAGGCATCCAagcg                                                     >  2:100414/1‑149 (MQ=255)
                                                                                          gATGGGTGGTGGTTTTGTGGGCCTCTTCGCGGGCCCGCCCGCTCCCACAGGTATAGCGCC‑ACATTCACGGTTGGCGCTACACCTGTGGTAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCAcgc                                                      <  6:290657/148‑1 (MQ=38)
                                                                                                  gtggtTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCGGTGGGAGCGGGCGTGCCCGCGAAGGAGTGAGTGAGGTCTAAGCGGCCGTGGCAGGCATCCACGCGCAACCCgc                                             >  8:46498/1‑149 (MQ=37)
                                                                                                    ggtTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTacac                                                                                                                        >  5:99900/1‑72 (MQ=255)
                                                                                                    ggtTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTacac                                                                                                                        <  6:99900/72‑1 (MQ=255)
                                                                                                         tGAGGGCCTCTTCGGGGGCACGCCGGCTCCCACTGGTTTAGCGCC‑ACATTCATGGTTGGCTATACACTTGTGGTAGGGGGGGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCca                                       <  2:8633/148‑1 (MQ=255)
                                                                                                                         ggCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGc                      <  8:259680/149‑1 (MQ=255)
                                                                                                                              gCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGTGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAg                 >  7:93240/1‑149 (MQ=255)
                                                                                                                                 cGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCAt              >  1:142938/1‑149 (MQ=255)
                                                                                                                                    tCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGaa           >  3:65445/1‑149 (MQ=255)
                                                                                                                                      ccACAGGTGTAGCGCCAACCTTGAATG‑TGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCa                  <  2:349376/140‑1 (MQ=21)
                                                                                                                                      ccACAGGTGTAGCGCCAACCTTGAATG‑TGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCa                  >  1:349376/1‑140 (MQ=21)
                                                                                                                                      ccACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc          >  8:162704/1‑148 (MQ=255)
                                                                                                                                      ccACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc          >  3:150284/1‑148 (MQ=255)
                                                                                                                                       cacaGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc          >  8:89180/1‑147 (MQ=255)
                                                                                                                                         caGGTGTAGCGCCAACCTTGAATG‑TGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc          >  7:354035/1‑145 (MQ=25)
                                                                                                                                            gTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACAAGCGCTCCAGCAGCTTGCAGCCTTGCACCAGCATGAACGAGATCa   >  8:322908/1‑149 (MQ=255)
                                                                                                                                             tataGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGTGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCAc  <  8:93240/149‑1 (MQ=255)
                                                                                                                                             tataGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCAc  >  1:158077/1‑149 (MQ=255)
                                                                                                                                              ataGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCAc  >  5:222802/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CCACAGCTGCTGGTGCTCGATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCAC  >  NC_002947/337667‑337956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑