Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,267,946 A→T 9.6% intergenic (‑89/+14) galP‑IV ← / ← PP_3740 porin‑like protein/MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,267,9460AT 9.6% 144.3 / 5.6 52intergenic (‑89/+14)galP‑IV/PP_3740porin‑like protein/MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (24/23);  new base T (3/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CAAACGCCAGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTGCCAGCCA  >  NC_002947/4267800‑4268089
                                                                                                                                                  |                                                                                                                                               
cAAACGCCAGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCTgg                                                                                                                                               >  6:265815/1‑149 (MQ=255)
        aGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGATGCATTCAGATACCCGGCCTGGCCGGGtat                                                                                                                                       <  5:265815/149‑3 (MQ=255)
          cGAAACAGCCAGGACGTAACGGGCCAGCGTGCAAGTTGTGTGCATCATTATTATTGTTCTCCTCGTGCAATAGGCGCAGCAGCCCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTaca                                                                                                                                     <  4:282195/149‑1 (MQ=255)
              aCAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACt                                                                                                                                   >  2:65516/1‑147 (MQ=255)
                aGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGt                                                                                                                               <  1:239839/149‑1 (MQ=255)
                aGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCAATCAGATACCCGGCCAGGCCGGGTTACACTCAGt                                                                                                                               <  2:360163/149‑1 (MQ=255)
                                 ccATCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATcagca                                                                                                              <  7:331660/149‑1 (MQ=255)
                                   aGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCagg                                                                                                            >  2:248690/1‑149 (MQ=255)
                                     cGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGt                                                                                                                       >  4:331532/1‑136 (MQ=255)
                                     cGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGt                                                                                                                       <  3:331532/136‑1 (MQ=255)
                                         cAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCaggcaagg                                                                                                       <  1:63588/148‑1 (MQ=255)
                                                     atcatTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCtt                                                                                          >  6:225616/1‑149 (MQ=255)
                                                      tcatTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTg                                                                                         >  4:183447/1‑149 (MQ=255)
                                                      tcatTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTg                                                                                         <  6:287411/149‑1 (MQ=255)
                                                         ttattattGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGcc                                                                                      <  4:1992/149‑1 (MQ=255)
                                                             tattGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTg                                                                                  >  4:22129/1‑149 (MQ=255)
                                                               ttGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTgg                                                                                 <  1:248690/148‑1 (MQ=255)
                                                                 gTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGgc                                                                               <  3:22129/148‑1 (MQ=255)
                                                                 cttCACCACTGTCAATAGCCGCAGCAGACCGCCCCACGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGgc                                                                               <  8:349836/147‑1 (MQ=255)
                                                                     tcCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCaa                                                                           >  4:184792/1‑148 (MQ=255)
                                                                     tacACGTTCAATAGGCGCAGAAGACCGCCCCAGGCCAGCATTACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGgcgcca                                                                           >  6:361255/3‑146 (MQ=255)
                                                                       cACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAc                                                                          <  3:9468/147‑1 (MQ=255)
                                                                       cACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAc                                                                          <  1:366008/147‑1 (MQ=255)
                                                                        aCGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCCGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAcag                                                                        >  6:165106/1‑148 (MQ=255)
                                                                          gTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAcagcag                                                                     >  4:133127/1‑149 (MQ=255)
                                                                          gTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAcagcag                                                                     >  5:182967/1‑149 (MQ=255)
                                                                             cAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAcagcagca                                                                   <  6:182967/148‑1 (MQ=255)
                                                                              aaTAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAgg                                                                 >  3:309619/1‑149 (MQ=255)
                                                                              aaTAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAgg                                                                 >  6:76586/1‑149 (MQ=255)
                                                                               aTAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCTGGcc                                                                                                                                             >  4:109306/1‑72 (MQ=255)
                                                                               aTAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCTGGcc                                                                                                                                             <  3:109306/72‑1 (MQ=255)
                                                                                   gcgcAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAAcc                                                            >  2:82980/1‑149 (MQ=255)
                                                                                       agcagACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCg                                                        <  3:184792/149‑1 (MQ=255)
                                                                                       agcagACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCg                                                        >  1:7243/1‑149 (MQ=255)
                                                                                              cGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCGGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCa                                                 >  1:167533/1‑149 (MQ=255)
                                                                                                 cccAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACg                                               >  1:182867/1‑148 (MQ=255)
                                                                                                    aGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAaca                                           <  3:133127/149‑1 (MQ=255)
                                                                                                           cATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCCGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGc                                    <  5:165106/149‑1 (MQ=255)
                                                                                                            aTGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGc                                    >  1:89604/1‑148 (MQ=255)
                                                                                                              gACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGaa                                 >  7:63830/1‑149 (MQ=255)
                                                                                                                                 cATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAg               <  3:183447/148‑1 (MQ=255)
                                                                                                                                  aTTCAGATACCCGGCCAGGCCTGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAggg             <  4:160079/149‑1 (MQ=255)
                                                                                                                                    tCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATa                        >  4:42256/1‑136 (MQ=255)
                                                                                                                                    tCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATa                        <  3:42256/136‑1 (MQ=255)
                                                                                                                                      aGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGTAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGtt          <  5:29987/148‑1 (MQ=255)
                                                                                                                                         tACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTgcca      <  1:275900/149‑1 (MQ=255)
                                                                                                                                          aCCCGGCCTGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTgccag     >  1:141771/1‑149 (MQ=255)
                                                                                                                                            ccGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGt           >  6:294777/1‑141 (MQ=255)
                                                                                                                                            ccGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGt           <  5:294777/141‑1 (MQ=255)
                                                                                                                                            ccGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTgccagcc   >  6:80299/1‑149 (MQ=255)
                                                                                                                                             cGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTgccagcca  >  4:3288/1‑149 (MQ=255)
                                                                                                                                             cGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTgccagcca  >  6:117335/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CAAACGCCAGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTGCCAGCCA  >  NC_002947/4267800‑4268089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑