Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,274,413 G→T 9.6% V124L (GTG→TTG)  glcD → glycolate oxidase FAD‑linked subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,274,4130GT 9.6% 181.8 / 5.3 60V124L (GTG→TTG) glcDglycolate oxidase FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base G (30/24);  new base T (2/4);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCACCAGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCC  >  NC_002947/4274267‑4274551
                                                                                                                                                         |                                                                                                                                          
gCCACCAGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGt                                                                                                                                           <  5:237629/148‑1 (MQ=255)
gCCACCAGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGt                                                                                                                                           <  2:271541/148‑1 (MQ=255)
 ccaccaGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTg                                                                                                                                          >  7:217583/1‑148 (MQ=255)
   accaGGGCGGCGTACCGGTGGGTGCGCGTGGCGCCGGGACCGGCCTGGCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCaa                                                                                                                                       <  4:231266/149‑1 (MQ=255)
   accaGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGGTTGCac                                                                                                                                       >  2:191066/1‑148 (MQ=255)
           ggggttccGGGGGTTGCGCGGGGGGCGGGTACCGGCCTGTCTGGGGGGGCCTCCCGCCGCTGCCTAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGc                                                                                                                                 <  1:120912/143‑1 (MQ=255)
             cgTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTAc                                                                                                                             <  3:356201/149‑1 (MQ=255)
             cgTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑‑TACGCCCGGTTGCACGCGGGCGTaac                                                                                                                            <  7:343113/149‑3 (MQ=255)
              gTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGGTTGCACGCGGGCGTaac                                                                                                                            <  2:257563/149‑3 (MQ=255)
              gTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGGTTGCACGCGGGCGTaac                                                                                                                            <  1:191066/149‑3 (MQ=255)
              gTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAACGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGGTTGCACGCGGGCGTaac                                                                                                                            <  2:56005/149‑3 (MQ=255)
                    gtggtTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCg                                                                                                                                <  7:302455/139‑1 (MQ=255)
                    gtggtTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCg                                                                                                                                >  8:302455/1‑139 (MQ=255)
                     tggtTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTg                                                                                                                     <  7:268931/149‑1 (MQ=255)
                       gtTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGc                                                                                                                   <  6:154001/149‑1 (MQ=255)
                            gcgTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCAtct                                                                                                              >  5:68543/1‑149 (MQ=255)
                              gTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTcc                                                                                                            <  8:110758/149‑1 (MQ=255)
                                            ggCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCTTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCAcc                                                                                              >  6:33982/1‑149 (MQ=255)
                                               cTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCCACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCa                                                                                           >  4:272080/1‑149 (MQ=255)
                                                  tCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAGGTGCCCAAGGGCGAAGAGTACGCCCGCGTGCAACCGGGCGTACCCACCCTGGCCATCTCCCAGGCCGCCGCAccc                                                                                             >  1:116741/1‑149 (MQ=255)
                                                      gtggtgC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCACGCGTGCAACAGGAAGTACGCAACCTCGCCATCTCCCAGGCCGCCGCACCCCATGGTATg                                                                                    >  6:155226/1‑149 (MQ=255)
                                                            c‑‑ccTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATgg                                                                                        >  5:190485/1‑139 (MQ=255)
                                                            c‑‑ccTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATgg                                                                                        <  6:190485/139‑1 (MQ=255)
                                                                     gCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGcc                                                                                                                  >  6:376165/1‑106 (MQ=255)
                                                                     gCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGcc                                                                                                                  <  5:376165/106‑1 (MQ=255)
                                                                         gCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGAccc                                                                   <  6:68543/149‑1 (MQ=255)
                                                                           cAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCa                                                                                                <  7:154408/118‑1 (MQ=255)
                                                                           cAAGGGCATCCTGATGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCa                                                                                                >  8:154408/1‑118 (MQ=255)
                                                                            aaGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTc                                                                >  8:200755/1‑149 (MQ=255)
                                                                             aGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTcc                                                               <  2:72735/149‑1 (MQ=255)
                                                                                gCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCg                                                            >  7:199116/1‑149 (MQ=255)
                                                                                  aTCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGc                                                           >  3:160684/1‑148 (MQ=255)
                                                                                  aTCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGAGAAACCGGGCCTACGCAACCAGGCCATATACCAAGCCCCCCCACCCCATGGTATTGACAACGCACCCGAACCATccaacc                                                           >  1:303795/1‑144 (MQ=255)
                                                                                   tCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCa                                                          >  2:200833/1‑148 (MQ=255)
                                                                                     ctgctgGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACACCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAAt                                                       >  6:95403/1‑149 (MQ=255)
                                                                                        ctgGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAAtt                                                      <  4:160684/147‑1 (MQ=255)
                                                                                            tGATGTCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGTCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGc                                                <  5:155226/149‑1 (MQ=255)
                                                                                                  cgcgcTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATc                                          >  5:242589/1‑149 (MQ=255)
                                                                                                     gcTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGt                                       <  6:242589/149‑1 (MQ=255)
                                                                                                     gcTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGt                                       <  1:200833/149‑1 (MQ=255)
                                                                                                       ttCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTgg                                     >  5:360550/1‑149 (MQ=255)
                                                                                                          aaCCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCaa                                  >  8:192921/1‑149 (MQ=255)
                                                                                                          aaCCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACACCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCaa                                  <  5:95403/149‑1 (MQ=255)
                                                                                                               tATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCg                             >  3:88877/1‑149 (MQ=255)
                                                                                                                   cTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCg                          >  1:106106/1‑148 (MQ=255)
                                                                                                                   cTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCg                          <  4:88877/148‑1 (MQ=255)
                                                                                                                    tCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGa                         >  1:246182/1‑148 (MQ=255)
                                                                                                                    tCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGACCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGa                         >  6:119105/1‑148 (MQ=255)
                                                                                                                         gTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGc                   >  4:308791/1‑149 (MQ=255)
                                                                                                                          tCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGcc                  >  5:99648/1‑149 (MQ=255)
                                                                                                                            a‑aCCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCgg                >  2:220949/1‑149 (MQ=255)
                                                                                                                            a‑aCCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCgg                >  2:271180/1‑149 (MQ=255)
                                                                                                                               cccGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGa                                            <  3:336911/119‑1 (MQ=255)
                                                                                                                               cccGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGa                                            >  4:336911/1‑119 (MQ=255)
                                                                                                                                 cGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCAc                                                                          <  3:60028/87‑1 (MQ=255)
                                                                                                                                 cGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCAc                                                                          >  4:60028/1‑87 (MQ=255)
                                                                                                                                  gCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCg           >  8:334852/1‑149 (MQ=255)
                                                                                                                                          g‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGc   >  5:184232/1‑149 (MQ=255)
                                                                                                                                          g‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATCGTATGTACTACGCTCCCGCCCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGc   <  2:116741/149‑1 (MQ=255)
                                                                                                                                               ttACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGcc  <  6:360550/149‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                          
GCCACCAGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGC‑‑CCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCA‑ACCCGCAGGGGCG‑‑‑‑TTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCC  >  NC_002947/4274267‑4274551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑