Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,305,604 G→A 7.3% R151C (CGC→TGC)  PP_3777 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,305,6040GA 7.3% 199.7 / 4.3 68R151C (CGC→TGC) PP_3777hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/29);  new base A (2/3);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

CCGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGAC  >  NC_002947/4305459‑4305748
                                                                                                                                                 |                                                                                                                                                
ccGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTg                                                                                                                                               <  1:67263/149‑1 (MQ=255)
ccGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTg                                                                                                                                               >  3:333579/1‑149 (MQ=255)
   gCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAg                                                                                                                                            <  7:61853/149‑1 (MQ=255)
         ccGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTTCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCAt                                                                                                                                       <  5:94711/148‑1 (MQ=255)
            aCCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGAg                                                                                                                                   <  4:153519/149‑1 (MQ=255)
             ccAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGGACCCGTTCTAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGGTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGAgg                                                                                                                                  <  8:105014/149‑1 (MQ=255)
             ccAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGAgg                                                                                                                                  <  5:19002/149‑1 (MQ=255)
              cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAgg                                                                                                                                  >  2:266208/1‑148 (MQ=255)
                aCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAg                                                                                                                               <  1:266208/149‑1 (MQ=255)
                aCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCAGCGAGGAAg                                                                                                                               >  1:142090/1‑149 (MQ=255)
                aCGAATTCAGCCCGATGATCAGCTTGCATCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACATGCGCAAGGTCCGCTCGCCATAGAACATCGATTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATAGATGAAg                                                                                                                               >  1:136478/1‑149 (MQ=255)
                     ttCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGt                                                                                                                                           >  1:270377/1‑132 (MQ=255)
                     ttCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGt                                                                                                                                           <  2:270377/132‑1 (MQ=255)
                      tCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATcc                                                                                                                         >  1:170325/1‑149 (MQ=255)
                       cAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATcc                                                                                                                         >  2:367089/1‑148 (MQ=255)
                        aGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATcc                                                                                                                         >  8:90927/1‑147 (MQ=255)
                         gCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAg                                                                                                                      >  1:256970/1‑149 (MQ=255)
                           ccGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAggg                                                                                                                    >  4:172300/1‑149 (MQ=255)
                                gatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGGAGTGCAGCGAGGAAGCCATCCGAGGGCGGCt                                                                                                               >  8:245551/1‑149 (MQ=255)
                                 atCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTg                                                                                                              <  6:321520/149‑1 (MQ=255)
                                      cTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                         <  7:138720/149‑1 (MQ=255)
                                          ccTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTgg                                                                                                     <  5:309150/149‑1 (MQ=255)
                                            tCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                   >  8:179711/1‑149 (MQ=255)
                                              cGGGCGTCAGGTAGCTCGGCACCCGTTCGAAAACCCTGCGCCAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACCGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                   <  3:223155/147‑1 (MQ=255)
                                                ggCTTCAGGTAGCTCGGCACCCGTTCCAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                               <  7:160729/149‑1 (MQ=255)
                                                   ttCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                            <  5:65911/149‑1 (MQ=255)
                                                     cAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                            <  4:144424/147‑1 (MQ=255)
                                                            cTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGTCACCCTGGCGGATTGAATAGGATca                                                                                    >  4:328085/1‑148 (MQ=255)
                                                               ggCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATc                                                                                <  5:281977/149‑1 (MQ=255)
                                                               ggCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATc                                                                                >  5:290827/1‑149 (MQ=255)
                                                                       ttCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGt                                                                        >  3:249049/1‑149 (MQ=255)
                                                                       ttCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGt                                                                        >  4:180004/1‑149 (MQ=255)
                                                                           aaaaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                    >  3:160589/1‑149 (MQ=255)
                                                                            aaaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTc                                                                       <  3:44496/145‑1 (MQ=255)
                                                                            aaaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTc                                                                       >  4:44496/1‑145 (MQ=255)
                                                                             aaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGAATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGTTTGTAATAGGATCACATAGTATCAGTCATCTg                                                                  >  8:275909/1‑149 (MQ=255)
                                                                              aaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGc                                                                 <  2:170325/149‑1 (MQ=255)
                                                                               aCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGcc                                                                <  2:256970/149‑1 (MQ=255)
                                                                                  tGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCg                                                               >  8:159731/1‑147 (MQ=255)
                                                                                    cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCAGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAgg                                                           >  2:84844/1‑149 (MQ=255)
                                                                                       aaGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                        >  2:205989/1‑149 (MQ=255)
                                                                                          gTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGc                                                     <  2:135530/149‑1 (MQ=255)
                                                                                             cGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTc                                                  <  1:205989/149‑1 (MQ=255)
                                                                                              gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                             >  3:262996/1‑137 (MQ=255)
                                                                                              gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                             <  4:262996/137‑1 (MQ=255)
                                                                                              gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTc                                                  >  5:346368/1‑148 (MQ=255)
                                                                                                  gCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                               >  6:306497/1‑147 (MQ=255)
                                                                                                      tAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAtc                                         <  4:333579/149‑1 (MQ=255)
                                                                                                           cATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCt                                    <  7:350086/149‑1 (MQ=255)
                                                                                                            aTCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGCTCTCCCTg                                   >  8:183802/1‑149 (MQ=255)
                                                                                                             tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                             >  5:48766/1‑122 (MQ=255)
                                                                                                             tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                             <  6:48766/122‑1 (MQ=255)
                                                                                                               gCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTa                                 <  4:160589/148‑1 (MQ=255)
                                                                                                                       cGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGa                        <  7:179711/149‑1 (MQ=255)
                                                                                                                          cAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                       <  2:251932/115‑1 (MQ=255)
                                                                                                                          cAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                       >  1:251932/1‑115 (MQ=255)
                                                                                                                           aGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACa                    <  7:90927/149‑1 (MQ=255)
                                                                                                                            gCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCAGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAg                   <  1:84844/149‑1 (MQ=255)
                                                                                                                                 tGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGGAGGCATTGCGTCGAACAGATCTCCCTGTAGGAATTCGATACGGGATTc              >  3:170906/1‑149 (MQ=255)
                                                                                                                                    gCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGcc           <  3:172300/149‑1 (MQ=255)
                                                                                                                                         cTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGt      >  4:175891/1‑149 (MQ=255)
                                                                                                                                         cTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGt      >  6:124414/1‑149 (MQ=255)
                                                                                                                                         cTCGATGCACTACAGCGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGt      >  2:308195/1‑149 (MQ=255)
                                                                                                                                         cTCGATGCACTACAGCGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGt      >  5:94116/1‑149 (MQ=255)
                                                                                                                                          tCGATGCGCGGTAGTGCATCGAGTAAGCCATCCGAGGGAGTCTGCCACCCTGGAGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGTCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATAAAGGATTCGCCCAGGtt     >  8:175919/1‑149 (MQ=255)
                                                                                                                                            gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGa   <  6:290827/149‑1 (MQ=255)
                                                                                                                                            gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGa   >  5:356257/1‑149 (MQ=255)
                                                                                                                                            gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCACGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGa   >  3:88329/1‑149 (MQ=255)
                                                                                                                                               gcgcTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGAc  >  7:61093/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGAC  >  NC_002947/4305459‑4305748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑