Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,305,608 T→C 7.5% L149L (CTA→CTG)  PP_3777 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,305,6080TC 7.5% 191.5 / 5.0 67L149L (CTA→CTG) PP_3777hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/28);  new base C (2/3);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

GCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGAC  >  NC_002947/4305462‑4305748
                                                                                                                                                  |                                                                                                                                            
gCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAg                                                                                                                                            <  7:61853/149‑1 (MQ=255)
      ccGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTTCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCAt                                                                                                                                       <  5:94711/148‑1 (MQ=255)
         aCCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGAg                                                                                                                                   <  4:153519/149‑1 (MQ=255)
          ccAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGGACCCGTTCTAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGGTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGAgg                                                                                                                                  <  8:105014/149‑1 (MQ=255)
          ccAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGAgg                                                                                                                                  <  5:19002/149‑1 (MQ=255)
           cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAgg                                                                                                                                  >  2:266208/1‑148 (MQ=255)
             aCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAg                                                                                                                               <  1:266208/149‑1 (MQ=255)
             aCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCAGCGAGGAAg                                                                                                                               >  1:142090/1‑149 (MQ=255)
             aCGAATTCAGCCCGATGATCAGCTTGCATCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACATGCGCAAGGTCCGCTCGCCATAGAACATCGATTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATAGATGAAg                                                                                                                               >  1:136478/1‑149 (MQ=255)
                  ttCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGt                                                                                                                                           >  1:270377/1‑132 (MQ=255)
                  ttCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGt                                                                                                                                           <  2:270377/132‑1 (MQ=255)
                   tCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATcc                                                                                                                         >  1:170325/1‑149 (MQ=255)
                    cAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATcc                                                                                                                         >  2:367089/1‑148 (MQ=255)
                     aGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATcc                                                                                                                         >  8:90927/1‑147 (MQ=255)
                      gCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAg                                                                                                                      >  1:256970/1‑149 (MQ=255)
                        ccGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAggg                                                                                                                    >  4:172300/1‑149 (MQ=255)
                             gatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGGAGTGCAGCGAGGAAGCCATCCGAGGGCGGCt                                                                                                               >  8:245551/1‑149 (MQ=255)
                              atCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTg                                                                                                              <  6:321520/149‑1 (MQ=255)
                                   cTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                         <  7:138720/149‑1 (MQ=255)
                                       ccTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTgg                                                                                                     <  5:309150/149‑1 (MQ=255)
                                         tCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                   >  8:179711/1‑149 (MQ=255)
                                           cGGGCGTCAGGTAGCTCGGCACCCGTTCGAAAACCCTGCGCCAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACCGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                   <  3:223155/147‑1 (MQ=255)
                                             ggCTTCAGGTAGCTCGGCACCCGTTCCAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                               <  7:160729/149‑1 (MQ=255)
                                                ttCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                            <  5:65911/149‑1 (MQ=255)
                                                  cAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                            <  4:144424/147‑1 (MQ=255)
                                                         cTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGTCACCCTGGCGGATTGAATAGGATca                                                                                    >  4:328085/1‑148 (MQ=255)
                                                            ggCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATc                                                                                <  5:281977/149‑1 (MQ=255)
                                                            ggCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATc                                                                                >  5:290827/1‑149 (MQ=255)
                                                                    ttCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGt                                                                        >  3:249049/1‑149 (MQ=255)
                                                                    ttCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGt                                                                        >  4:180004/1‑149 (MQ=255)
                                                                        aaaaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                    >  3:160589/1‑149 (MQ=255)
                                                                         aaaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTc                                                                       <  3:44496/145‑1 (MQ=255)
                                                                         aaaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTc                                                                       >  4:44496/1‑145 (MQ=255)
                                                                          aaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGAATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGTTTGTAATAGGATCACATAGTATCAGTCATCTg                                                                  >  8:275909/1‑149 (MQ=255)
                                                                           aaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGc                                                                 <  2:170325/149‑1 (MQ=255)
                                                                            aCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGcc                                                                <  2:256970/149‑1 (MQ=255)
                                                                               tGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCg                                                               >  8:159731/1‑147 (MQ=255)
                                                                                 cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCAGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAgg                                                           >  2:84844/1‑149 (MQ=255)
                                                                                    aaGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                        >  2:205989/1‑149 (MQ=255)
                                                                                       gTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGc                                                     <  2:135530/149‑1 (MQ=255)
                                                                                          cGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTc                                                  <  1:205989/149‑1 (MQ=255)
                                                                                           gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                             >  3:262996/1‑137 (MQ=255)
                                                                                           gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                             <  4:262996/137‑1 (MQ=255)
                                                                                           gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTc                                                  >  5:346368/1‑148 (MQ=255)
                                                                                               gCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                               >  6:306497/1‑147 (MQ=255)
                                                                                                   tAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAtc                                         <  4:333579/149‑1 (MQ=255)
                                                                                                        cATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCt                                    <  7:350086/149‑1 (MQ=255)
                                                                                                         aTCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGCTCTCCCTg                                   >  8:183802/1‑149 (MQ=255)
                                                                                                          tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                             >  5:48766/1‑122 (MQ=255)
                                                                                                          tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                             <  6:48766/122‑1 (MQ=255)
                                                                                                            gCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTa                                 <  4:160589/148‑1 (MQ=255)
                                                                                                                    cGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGa                        <  7:179711/149‑1 (MQ=255)
                                                                                                                       cAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                       <  2:251932/115‑1 (MQ=255)
                                                                                                                       cAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                       >  1:251932/1‑115 (MQ=255)
                                                                                                                        aGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACa                    <  7:90927/149‑1 (MQ=255)
                                                                                                                         gCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCAGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAg                   <  1:84844/149‑1 (MQ=255)
                                                                                                                              tGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGGAGGCATTGCGTCGAACAGATCTCCCTGTAGGAATTCGATACGGGATTc              >  3:170906/1‑149 (MQ=255)
                                                                                                                                 gCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGcc           <  3:172300/149‑1 (MQ=255)
                                                                                                                                      cTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGt      >  4:175891/1‑149 (MQ=255)
                                                                                                                                      cTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGt      >  6:124414/1‑149 (MQ=255)
                                                                                                                                      cTCGATGCACTACAGCGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGt      >  5:94116/1‑149 (MQ=255)
                                                                                                                                      cTCGATGCACTACAGCGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGt      >  2:308195/1‑149 (MQ=255)
                                                                                                                                       tCGATGCGCGGTAGTGCATCGAGTAAGCCATCCGAGGGAGTCTGCCACCCTGGAGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGTCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATAAAGGATTCGCCCAGGtt     >  8:175919/1‑149 (MQ=255)
                                                                                                                                         gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGa   <  6:290827/149‑1 (MQ=255)
                                                                                                                                         gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGa   >  5:356257/1‑149 (MQ=255)
                                                                                                                                         gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCACGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGa   >  3:88329/1‑149 (MQ=255)
                                                                                                                                            gcgcTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGAc  >  7:61093/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
GCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGAC  >  NC_002947/4305462‑4305748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑