Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,338,404	G→A	10.9%	intergenic (+24/+118)	PP_3812 → / ← PP_5632	lipase/allo‑isoleucine metabolism protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,338,404	0	G	A	10.9%	144.8 / 8.2	55	intergenic (+24/+118)	PP_3812/PP_5632	lipase/allo‑isoleucine metabolism protein
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base A (3/3);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

TAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAAT  >  NC_002947/4338256‑4338545                                                                                                                                                     |                                                                                                                                               tAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTg                                                                                                                                                >  5:238884/1‑149 (MQ=255)     cTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCg                                                                                                                                            <  7:68529/149‑1 (MQ=255)        ggTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCCAGGCGGGt                                                                                                                                         <  5:334132/149‑1 (MQ=255)               tACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGc                                                                                                                                  >  2:214433/1‑149 (MQ=255)                aCAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTg                                                                                                                                   >  7:349485/1‑147 (MQ=255)                aCAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTg                                                                                                                                   <  8:349485/147‑1 (MQ=255)                   aCGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTc                                                                                                                               >  5:210516/1‑148 (MQ=255)                   aCGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATACACCCGCCCAGGCGGGTCTATTGCtcat                                                                                                                              <  1:11158/149‑4 (MQ=255)                      gAATGATTCATGACTATGGGCTGCTC‑ACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTAttt                                                                                                                          >  2:344111/1‑149 (MQ=255)                       aaTGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTAttt                                                                                                                          >  1:199904/1‑149 (MQ=255)                              cATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTGGCTTCTATTTGCGGaaa                                                                                                                   >  6:307932/1‑149 (MQ=255)                                   cTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGcc                                                                                                              <  2:97402/149‑1 (MQ=255)                                    tATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGccc                                                                                                             >  4:29101/1‑149 (MQ=255)                                    tATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGccc                                                                                                             <  7:335298/149‑1 (MQ=255)                                             ctCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCtt                                                                                                     >  1:269440/1‑148 (MQ=255)                                             ctCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCtt                                                                                                     <  6:194960/148‑1 (MQ=255)                                              tCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAGCTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTc                                                                                                    >  5:348096/1‑148 (MQ=255)                                              tCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTc                                                                                                    >  6:69974/1‑148 (MQ=255)                                                aaCCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAAt                                                                                                 >  5:153362/1‑149 (MQ=255)                                                aaCCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAAt                                                                                                 <  1:214433/149‑1 (MQ=255)                                                aaCCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTAAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAAt                                                                                                 <  7:141588/149‑1 (MQ=255)                                                   ccGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGaa                                                                                              <  8:123380/149‑1 (MQ=255)                                                             aaGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCg                                                                                    >  4:295261/1‑149 (MQ=255)                                                              aGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCgg                                                                                   >  1:192805/1‑149 (MQ=255)                                                                tGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTg                                                                                 <  2:192805/149‑1 (MQ=255)                                                                  ccGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTc                                                                                                                               >  6:178770/1‑101 (MQ=255)                                                                  ccGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTc                                                                                                                               <  5:178770/101‑1 (MQ=255)                                                                  ccGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGc                                                                                <  6:238884/148‑1 (MQ=255)                                                                    ggCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑Gc                                                                            <  6:210516/149‑1 (MQ=255)                                                                       aGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGc                                                                                >  7:309526/1‑143 (MQ=255)                                                                                 gcgcTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCtt                                                               >  2:306585/1‑149 (MQ=255)                                                                                     tGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGc                                                                                                                        >  8:339035/1‑89 (MQ=255)                                                                                     tGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGc                                                                                                                        <  7:339035/89‑1 (MQ=255)                                                                                            ggCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCATTAGCGGGTaaa                                                    >  3:100129/1‑149 (MQ=255)                                                                                              cGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAAcc                                                  <  2:199904/149‑1 (MQ=255)                                                                                                      gAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTTTACCCGCGAAAg                                          <  6:153362/149‑1 (MQ=255)                                                                                                      gAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAg                                          <  3:295261/149‑1 (MQ=255)                                                                                                        aCTTAAAGTGCATCTGCAGTGAGGCCATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGTGCAGGTCCGGCCCTTTCGCGGGTTTACCCGCGAAAgg                                         <  1:67460/149‑1 (MQ=255)                                                                                                         cTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCATTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTTTACCCGCGAAAGGGc                                       <  1:306585/149‑1 (MQ=255)                                                                                                           tAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCATTAGCCGGGAAAACCCCGAAAAGGCCg                                     >  5:1833/1‑149 (MQ=255)                                                                                                            aaaGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCgg                                    >  8:259726/1‑149 (MQ=255)                                                                                                             aaGTGCATCTGCAGTGAGGCGATCAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATCAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCgg                                    <  3:246799/148‑1 (MQ=255)                                                                                                                          gTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAg                      <  5:69974/149‑1 (MQ=255)                                                                                                                          gTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAg                      <  2:269440/149‑1 (MQ=255)                                                                                                                                atagaaGCAAAAGACCCGCCCAGGCGGGTCTATTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAgg                                         <  3:46121/119‑1 (MQ=38)                                                                                                                                atagaaGCAAAAGACCCGCCCAGGCGGGTCTATTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAgg                                         >  4:46121/6‑124 (MQ=38)                                                                                                                                      gCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCt          >  2:95748/1‑149 (MQ=255)                                                                                                                                       cAAAAGACCCGCCCAGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCAATAGCGGGTAAACCCCCGAAAGGGCCCGGAAATACTGCACAGGGCTCAAACCCta         >  4:349535/1‑148 (MQ=255)                                                                                                                                       cAAAAGACCCGCCCAGGCGGGTCTATTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCtt         >  3:121189/1‑149 (MQ=255)                                                                                                                                        aaTAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTc        >  5:368304/1‑149 (MQ=255)                                                                                                                                        aaTAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTc        >  2:50111/1‑149 (MQ=255)                                                                                                                                        aaTAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTc        <  1:95748/149‑1 (MQ=255)                                                                                                                                         aTAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGTTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTcc       <  1:344111/149‑1 (MQ=255)                                                                                                                                             aCCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTAGCGGGTTAACCCGCCAAACGGCCCGCAAAACCGGCTCAGGGCTCAAACCCATCcaca    >  2:327237/1‑148 (MQ=255)                                                                                                                                               ccGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAAt  >  1:243463/1‑148 (MQ=255)                                                                                                                                               ccGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCATTCGCGGGGGAAACCGCGAAACGGCCGGCACATACAGGTCAGGGCGGAAACCCGGCcaca    >  1:157397/1‑146 (MQ=255)                                                                                                                                                     |                                                                                                                                               TAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCG‑GCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAAT  >  NC_002947/4338256‑4338545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑