| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,372,569 | G→A | 6.6% | intergenic (‑1154/‑418) | PP_5636 ← / → PP_3849 | hypothetical protein/hemolysin‑type calcium‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,372,569 | 0 | G | A | 6.6% | 173.8 / 2.3 | 60 | intergenic (‑1154/‑418) | PP_5636/PP_3849 | hypothetical protein/hemolysin‑type calcium‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (30/26); new base A (2/2); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01 | |||||||||||
CATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCGGGGTTAATGACTCTTGGA > NC_002947/4372422‑4372711 | cATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACgg > 7:142755/1‑149 (MQ=255) cGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGAtt > 5:106379/1‑149 (MQ=255) aTTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGAt > 8:202346/1‑140 (MQ=255) aTTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGAt < 7:202346/140‑1 (MQ=255) ttATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCGCCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATc > 1:220730/1‑146 (MQ=255) gATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACAt > 7:190361/1‑149 (MQ=255) tAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCACTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATc < 8:142755/148‑1 (MQ=255) aaTCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTa < 7:223826/149‑1 (MQ=255) aaTCATCAATCAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTa < 4:73780/149‑1 (MQ=255) tcatcaATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAgt < 6:250427/149‑1 (MQ=255) catcaATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAgtg > 4:248712/1‑149 (MQ=255) tcaATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGcc > 1:24548/1‑111 (MQ=255) tcaATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGcc < 2:24548/111‑1 (MQ=255) tcaATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAgtgt > 3:196375/1‑148 (MQ=255) aaagAGCCCCGCGCCAGACACTACCGCACCCAAACTGTCCACACTAACAATACCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCAt < 6:370816/146‑1 (MQ=255) aaTGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCAt > 1:184849/1‑149 (MQ=255) tGAGACACGCTCCAGACACTACCGCACCCAAATTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCAttt < 8:149092/149‑1 (MQ=255) ccACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCAGGTCTTACAAAACACGGCCAAGCCACGGACGATTTATATGGCATCAAGAACATCGTAGTGTCATTTGa > 7:145767/1‑147 (MQ=255) ccACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGAAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAACCCAAGGACGATTTAGCTGGCATCAAGAACATCGATGTGTCATTTGa > 6:291046/1‑147 (MQ=255) cGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACAGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGa > 1:2622/1‑148 (MQ=255) cGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACAGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGa > 1:8670/1‑148 (MQ=255) cAGACACTACCGCACCCAAACTGTCATCCCTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCCCGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTg > 2:228491/1‑149 (MQ=255) gACACTACCGCCCCCAAACTGGCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGc < 8:352719/149‑1 (MQ=255) gACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGGCATTTGACAGAAATCTGGc > 3:44445/1‑149 (MQ=255) acTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCtt > 8:213821/1‑148 (MQ=255) ccGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACATTAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTc < 6:142456/148‑1 (MQ=255) cACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCata > 3:100630/1‑148 (MQ=255) ccAAACTGTCATCACTAACAATTACCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATac > 7:51589/1‑148 (MQ=255) aaaCTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACt < 8:1805/149‑1 (MQ=255) aaaCTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACt < 2:220730/149‑1 (MQ=255) aaCTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTc > 4:39059/1‑139 (MQ=255) aaCTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTc < 3:39059/139‑1 (MQ=255) ttatgaCTAACAATTCCCTCGATAGTCAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCTAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATg < 4:44445/143‑1 (MQ=255) atcaCTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACa > 6:241906/1‑149 (MQ=255) cTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTa > 7:124674/1‑149 (MQ=255) aaCAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACg > 3:37848/1‑149 (MQ=255) aCAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAAt < 3:12260/138‑1 (MQ=255) aCAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAAt > 4:12260/1‑138 (MQ=255) aCAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGc > 1:59216/1‑149 (MQ=255) cccTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCgaga < 8:190361/148‑1 (MQ=255) cccTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCgag > 2:244533/1‑147 (MQ=255) cTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGagaaga > 8:198518/1‑149 (MQ=255) gATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACg < 7:198518/148‑1 (MQ=255) aTAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGaa < 4:196375/149‑1 (MQ=255) tAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAAc < 7:213821/149‑1 (MQ=255) tAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATc > 6:332094/1‑149 (MQ=255) aaTCTTTCGTCAGGGCGGGCTTGTCTTACAATAGACGGCCAAGCGACGGAGGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATcc < 8:145767/148‑1 (MQ=255) tCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGt < 5:332094/147‑1 (MQ=255) gggcgggcTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCg < 4:37848/149‑1 (MQ=255) gggcgggcTGGTCTTACAATAGACAGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCg < 2:8670/149‑1 (MQ=255) gggcgggcTGGTCTTACAATAGACAGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCg < 2:2622/149‑1 (MQ=255) ggcgggcTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCgg < 3:248712/149‑1 (MQ=255) cgggcTGGTCTTACAATAGACGGCCACGCCCCGGACGATTTATCTGGCATCAAGAACATCGTAGTGGCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCAATTGGAAAGGGCgggg > 2:117363/1‑149 (MQ=255) gcTGGACTTACAATAGACGGACAAGCCACGGACGATTTATCTTGCATCAAGAACATCGTAGTCTCATTTGACAGAAATCTGGCTAACTAATATACACTGAAAGACACTTACGCCGAGAAGACGAACATCCATAGTAAATGGAGGGGtt > 8:281757/1‑148 (MQ=255) tGGTCTTACAATAGACGGCCAAGCCACTGACGATTTAGATGGCATCAAGAACATCGTTGTGTCATTTGACAGTTTTCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGCCGAACCTCCATTGTAAATGGCGGGGTTAAt < 1:117363/149‑1 (MQ=255) tGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGATGAACATCCATTGTAAATGGCGGGGTTAAt > 7:166633/1‑149 (MQ=255) cAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCGGGGTTAATGACTCtt < 4:100630/148‑1 (MQ=255) cAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCGGGGTTAATGACTCTTg < 2:184849/149‑1 (MQ=255) aaTAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCGGGGTTAATGACTCTTg > 6:28554/1‑148 (MQ=255) tAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCGGGGTTAATGACTCTTGGa > 8:212519/1‑148 (MQ=255) gACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCGGGGTTAATGACTCTTGGa > 5:304142/1‑146 (MQ=255) | CATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCTGGCATCAAGAACATCGTAGTGTCATTTGACAGAAATCTGGCTTACTCATATACACTGAATGACAGTTACGCCGAGAAGACGAACATCCATTGTAAATGGCGGGGTTAATGACTCTTGGA > NC_002947/4372422‑4372711 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |