| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,431,526 | T→C | 7.4% | A64A (GCT→GCC) | PP_3926 → | short‑chain dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,431,526 | 0 | T | C | 7.4% | 155.7 / 2.6 | 54 | A64A (GCT→GCC) | PP_3926 | short‑chain dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (19/31); new base C (2/2); total (21/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
TTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTCAACAATGCAGCCACCAACCCGCAATTCTGCAATGTGCTGGACACCGACCCAGGG > NC_002947/4431381‑4431667 | cttgcGGCGCCTGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCCGCAAGGGGCCCTGGTGATCGTTTCCAGCCTCAAGCTTGCCGGGGGCCAGCAGTTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCtgt < 5:5264/144‑1 (MQ=255) cgcCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACAGCCGTGGCCttgc < 3:88610/149‑4 (MQ=255) cgcCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTCACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACAGCCGTGGCCttgc > 4:88610/1‑146 (MQ=255) gcCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCca > 2:277503/1‑149 (MQ=255) gCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACAtt > 1:159090/1‑149 (MQ=255) ccGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTg < 1:277503/149‑1 (MQ=255) ccGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGGGCCAGCCGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTg < 5:248163/149‑1 (MQ=255) gTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGt > 1:361430/1‑149 (MQ=255) tGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAATCTTGACGGGTGCCAGCAGGTGGCCGACGCCACT‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGt < 1:82157/149‑1 (MQ=255) tGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑TCCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGt < 1:77541/149‑1 (MQ=255) tGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGt > 6:2717/1‑148 (MQ=255) tGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTg < 8:187452/149‑1 (MQ=255) ctCGGCGAGGCCATCGCCCTCTTGCTCGCGCAGCAAGGGGCGCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCt < 8:60319/148‑1 (MQ=255) aTCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCt < 6:38850/149‑1 (MQ=255) aTCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCt > 4:32429/1‑149 (MQ=255) aTCGGCGAGGCCATCGCCCACTTCCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCCCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACAGCCGTGGCCtt < 7:296866/134‑2 (MQ=255) ggggcttggCCATCGCCCACTTGCTCGCGCAGAAAGGGGCCCATGGTAGCGTTTCCAGCCGCACGCTTGACGGGGGCCAGCAGGGGCCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTg < 2:159090/142‑1 (MQ=255) ggCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTgg < 6:225118/148‑1 (MQ=255) ggCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGa < 1:170931/149‑1 (MQ=255) gCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGAt > 8:17576/1‑149 (MQ=255) cATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGTGGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTc < 3:86061/149‑1 (MQ=255) cATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGAtt > 1:139813/1‑148 (MQ=255) gCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTcagca < 1:25048/149‑1 (MQ=255) cccACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTcagcag < 5:12448/149‑1 (MQ=255) cccACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTcagcag > 6:12448/1‑149 (MQ=255) ccACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAgg < 3:32429/149‑1 (MQ=255) ccACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAgg > 4:364055/1‑149 (MQ=255) ttGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGtt > 7:158735/1‑149 (MQ=255) ccgcgcgCAGCATGGGGCCCATGTGATCTTTTCCCCCCGCACGCTTGCGGGGGGCCCGCCGGTGCCCGACGCCA‑T‑CTTTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTc < 2:361430/145‑1 (MQ=255) tCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGa > 5:179793/1‑132 (MQ=255) tCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGa < 6:179793/132‑1 (MQ=255) gcgcAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGcc < 3:135879/147‑1 (MQ=255) gcgcAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCgg > 2:176582/1‑149 (MQ=255) gcagcaAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCa > 5:82641/1‑149 (MQ=255) aaGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTcgc < 3:239586/149‑1 (MQ=255) aGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTcgcg < 6:179826/149‑1 (MQ=255) ggggCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAg > 8:109508/1‑111 (MQ=255) ggggCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAg < 7:109508/111‑1 (MQ=255) gggCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGaa > 6:159770/1‑149 (MQ=255) ggCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAAc < 3:207353/149‑1 (MQ=255) gtgATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGtt > 7:135787/1‑146 (MQ=255) gtgATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGtt < 8:135787/146‑1 (MQ=255) tgATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCggg < 8:158735/149‑1 (MQ=255) gTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGGGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGAc < 3:154281/148‑1 (MQ=255) aGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGt > 8:176949/1‑149 (MQ=255) gCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTg < 3:173291/149‑1 (MQ=255) gCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTg > 4:173291/1‑149 (MQ=255) gCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTg < 3:364055/149‑1 (MQ=255) gCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGCTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTg < 2:139813/149‑1 (MQ=255) aaGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGt < 2:131881/149‑1 (MQ=255) tGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTCAACAATGCAGc < 5:2717/149‑1 (MQ=255) ccGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTCAACAATGCAGCCACCAACCCGCaa < 3:50864/149‑1 (MQ=255) ca‑t‑catTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTCAACAATGAAGCCACCAACCCGCAATTCTGc > 6:307139/1‑148 (MQ=255) ggCCACAGCCGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTCAACAATGCAGCCACCAACCCGCAATTCTGCAATGTGCTGGACAccccagc > 4:272728/1‑142 (MQ=255) ccACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTCAACAATGCAGCCACCAACCCGCAATTCTGCAATGTGCTGGACACCGACCCAggg < 1:176582/149‑1 (MQ=255) | TTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCA‑T‑CATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTCAACAATGCAGCCACCAACCCGCAATTCTGCAATGTGCTGGACACCGACCCAGGG > NC_002947/4431381‑4431667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |