Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,463,959 A→T 8.2% intergenic (+6/+22) PP_3956 → / ← betT‑III hypothetical protein/choline/carnitine/betaine transporter BetTC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,463,9590AT 8.2% 167.6 / 5.3 61intergenic (+6/+22)PP_3956/betT‑IIIhypothetical protein/choline/carnitine/betaine transporter BetTC
Reads supporting (aligned to +/- strand):  ref base A (25/31);  new base T (3/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01

ACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTTCCGC  >  NC_002947/4463817‑4464106
                                                                                                                                              |                                                                                                                                                   
aCTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAggg                                                                                                                                                >  5:345471/1‑148 (MQ=255)
 cTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGTCGCCGTCAGGGCa                                                                                                                                              <  3:330029/149‑1 (MQ=255)
 cTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCa                                                                                                                                              <  5:191970/149‑1 (MQ=255)
  tACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAg                                                                                                                                             >  3:166695/1‑149 (MQ=255)
    ccAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCa                                                                                                                                              <  8:355640/146‑1 (MQ=255)
    ccAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCa                                                                                                                                              >  7:355640/1‑146 (MQ=255)
    ccAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAg                                                                                                                                             <  3:291633/147‑1 (MQ=255)
      aGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGAcc                                                                                                                                         <  2:84700/149‑1 (MQ=255)
         tCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGaa                                                                                                                                      >  5:353520/1‑149 (MQ=255)
             cACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCa                                                                                                                                  >  5:337725/1‑149 (MQ=255)
             cACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCa                                                                                                                                  >  2:142455/1‑149 (MQ=255)
              aCGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGAc                                                                                                                                                    >  6:50493/1‑130 (MQ=255)
              aCGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGAc                                                                                                                                                    <  5:50493/130‑1 (MQ=255)
               cGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACt                                                                                                                                <  6:353520/149‑1 (MQ=255)
                gACAGACGGGTGGCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAAGGCTTTGCTGCAGTAGACTTCTTGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACtt                                                                                                                               <  4:361961/149‑1 (MQ=255)
                   aGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAg                                                                                                                            <  7:99001/149‑1 (MQ=255)
                   aGACAGGTGCCAGCCACTGTCCTAGTGGTTAGGACCTCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCATGCCCTGAAGGCGACAGGGCAGGACCGAATCCACTTCAg                                                                                                                            >  5:364214/1‑149 (MQ=255)
                      cGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAAACCACTTCAGcc                                                                                                                          >  7:136083/1‑148 (MQ=255)
                              agccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTg                                                                                                                 >  1:311432/1‑149 (MQ=255)
                                 caCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCaa                                                                                                              <  3:275760/149‑1 (MQ=255)
                                   cTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGt                                                                                                            <  8:90949/149‑1 (MQ=255)
                                      tCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTaa                                                                                                          <  1:142455/148‑1 (MQ=255)
                                      tCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAAt                                                                                                         >  8:195694/1‑149 (MQ=255)
                                        cTATTGGTTTGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGc                                                                                                       <  5:200398/149‑1 (MQ=255)
                                        cTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAg                                                                                                                            >  6:293628/1‑128 (MQ=255)
                                        cTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAg                                                                                                                            <  5:293628/128‑1 (MQ=255)
                                             gcttAGGACCGCCGAGCGAACGCATTGCTCCAGTAGACTTCTGGGCCTGTGAAACTCCGCCTTTGGACAGCAGGCGATCCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGt                                                                                                   <  4:54048/146‑1 (MQ=255)
                                              gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAg                                                                                                             <  6:50804/137‑1 (MQ=255)
                                              gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAg                                                                                                             >  5:50804/1‑137 (MQ=255)
                                                   gACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTc                                                                                            <  5:140438/149‑1 (MQ=255)
                                                    aCCGCCGAGCTTACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCg                                                                                           <  1:317015/149‑1 (MQ=255)
                                                     ccgccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCg                                                                                           >  2:161007/1‑148 (MQ=255)
                                                      cgccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTa                                                                                         <  7:195694/149‑1 (MQ=255)
                                                        ccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGTCGCCGTCAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACt                                                                                       >  1:17055/1‑149 (MQ=255)
                                                        ccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGGAATGCAGGTGGCGTTCGTACt                                                                                       >  8:64080/1‑149 (MQ=255)
                                                                aCGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAgg                                                                               <  5:258187/149‑1 (MQ=255)
                                                                 cGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGa                                                                              >  5:213499/1‑149 (MQ=255)
                                                                  gCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTa                                                                                         <  5:223419/137‑1 (MQ=255)
                                                                  gCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTa                                                                                         >  6:223419/1‑137 (MQ=255)
                                                                           cAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACATGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAAt                                                                    >  6:257426/1‑149 (MQ=255)
                                                                             gTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACACGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATca                                                                  <  2:111268/149‑1 (MQ=255)
                                                                                gACTTATGGGCCTGTGAAAATCCGCCTTTGGACAGCATGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGAGCCAGGATGTCGCCAATcaca                                                                <  6:364214/148‑1 (MQ=255)
                                                                                       gggCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACg                                                        >  2:228158/1‑149 (MQ=255)
                                                                                        ggCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACATGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACgg                                                       <  5:257426/149‑1 (MQ=255)
                                                                                           cTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTc                                                    <  4:166695/149‑1 (MQ=255)
                                                                                             gtgAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCa                                                  >  1:261781/1‑149 (MQ=255)
                                                                                              tgAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCAc                                                 >  2:100316/1‑149 (MQ=255)
                                                                                                aaaaTCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCAcc                                                <  6:213499/148‑1 (MQ=255)
                                                                                                  aaTCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCa                                             <  7:356147/149‑1 (MQ=255)
                                                                                                    tCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAAGGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCGAATCACATCGTCACGGCTCCAccccgt                                            >  4:262621/1‑146 (MQ=255)
                                                                                                         cTTTGGACAGCAGGCGAACCGCCCTGCCCTGTCGCCGTCAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTc                                      <  2:17055/149‑1 (MQ=255)
                                                                                                         cTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCGGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTc                                      <  2:126288/149‑1 (MQ=255)
                                                                                                            tGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCg                                     <  2:311432/147‑1 (MQ=255)
                                                                                                               aCAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTc                                >  1:177014/1‑149 (MQ=255)
                                                                                                                         ttcggtcctgccctgTCGCCGTCAGGGCAGGGCCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCt                      >  4:298748/7‑149 (MQ=255)
                                                                                                                             gtcctgccctgTCGCCGTCAGGGCAGGGCCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTtctc                  >  6:142023/3‑149 (MQ=255)
                                                                                                                              ccctgccctgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCg                 <  2:177014/149‑1 (MQ=255)
                                                                                                                                      tgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGcccc                        <  2:332111/134‑1 (MQ=255)
                                                                                                                                      tgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGcccc                        >  1:332111/1‑134 (MQ=255)
                                                                                                                                        aCGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCAc        <  5:338583/148‑1 (MQ=255)
                                                                                                                                             gACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTTCcgc  >  3:368105/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
ACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTTCCGC  >  NC_002947/4463817‑4464106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑