Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,470,708 A→G 5.2% V161A (GTG→GCG)  PP_3961 ← transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,470,7080AG 5.2% 230.9 / 2.0 77V161A (GTG→GCG) PP_3961transporter
Reads supporting (aligned to +/- strand):  ref base A (33/40);  new base G (2/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCGACATGCTGTCTTGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGCCGACATGCTGGGCATGAAGCCGGCGA  >  NC_002947/4470562‑4470852
                                                                                                                                                   |                                                                                                                                                   
ccGACATGCTGTCTTGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCAcc                                                                                                                                                   <  1:228220/149‑1 (MQ=255)
 cGACATGCTGTCTTGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCAc                                                                                                                                                    <  4:289762/147‑1 (MQ=255)
      tGCTGTCTTGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATc                                                                                                                                             <  1:231972/149‑1 (MQ=255)
        cTGTCTTGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATcgg                                                                                                                                           <  1:295088/149‑1 (MQ=255)
           tCTTGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATcggcg                                                                                                                                         <  6:356064/148‑1 (MQ=255)
           tCTTGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATcggc                                                                                                                                          <  4:333024/147‑1 (MQ=255)
             ttGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGCTGTACCGCCAC‑GCTCAGCCCCAGCGCACTCGGCGGGc                                                                                                                                      >  8:120531/1‑149 (MQ=255)
             ttGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGc                                                                                                                                      >  1:182880/1‑149 (MQ=255)
                cccGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCGCCGATTTCGGTggggct                                                                                                                                    >  2:160794/1‑145 (MQ=255)
                cccGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑g                                                                                                                                  <  7:352996/149‑1 (MQ=255)
                 ccGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGTGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑g                                                                                                                                  >  5:284062/1‑148 (MQ=255)
                 ccGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCGCCGATTTCGGTggggctga                                                                                                                                  <  1:160794/149‑6 (MQ=255)
                 ccGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATcg                                                                                                                                            >  1:282419/1‑139 (MQ=255)
                 ccGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATcg                                                                                                                                            <  2:282419/139‑1 (MQ=255)
                    gTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑At                                                                                                                             <  5:344121/149‑1 (MQ=255)
                    gTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑At                                                                                                                             <  4:361745/149‑1 (MQ=255)
                     tGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑At                                                                                                                             >  2:11942/1‑148 (MQ=255)
                       gcggcATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGaa                                                                                                                          <  7:165060/149‑1 (MQ=255)
                          gcATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAgg                                                                                                                        >  3:93999/1‑148 (MQ=255)
                          gcATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAgg                                                                                                                        <  4:93999/148‑1 (MQ=255)
                          gcATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGCAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGt                                                                                                                       <  4:74745/149‑1 (MQ=255)
                                gCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTc                                                                                                                <  2:182880/149‑1 (MQ=255)
                                  ctctACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCg                                                                                                               >  3:270615/1‑148 (MQ=255)
                                     tACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTc                                                                                                             <  2:22402/147‑1 (MQ=255)
                                        tGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGaa                                                                                                        >  4:198263/1‑149 (MQ=255)
                                          gcggcTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGaa                                                                                                        <  7:64563/147‑1 (MQ=255)
                                              cTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGttt                                                                                                  >  3:17793/1‑149 (MQ=255)
                                                 ggTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTc                                                                                                 <  3:198263/147‑1 (MQ=255)
                                                  gTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAg                                                                                              >  3:254272/1‑149 (MQ=255)
                                                   tgcgtaCCTGCACCGCAATGCCTAACACGCCTATGGCATGTAACACGCAAGCTGCCAATGAACCGGCCTGCGTATGTACCGCCACAGCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAg                                                                                              <  1:111432/144‑1 (MQ=255)
                                                   tGCCGACCTGCACCGCCAAGGCTAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGc                                                                                             <  4:270615/149‑1 (MQ=255)
                                                   tGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGc                                                                                             <  4:86241/149‑1 (MQ=255)
                                                    gCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGc                                                                                             >  2:71382/1‑148 (MQ=255)
                                                       gACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGt                                                                                         <  4:272133/149‑1 (MQ=255)
                                                          cTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAgg                                                                                      >  3:90150/1‑149 (MQ=255)
                                                           tGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGc                                                                                     >  7:122564/1‑149 (MQ=255)
                                                            gCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCt                                                                                    >  8:258757/1‑149 (MQ=255)
                                                             gaCCGCCATGGCGAACACGCCGATGGGCATGAACACGGCATGAGCCAATGTACCGGCCTGCGGAAGTACCTGCAC‑GCTCAGCCCCACCGAAATCGGCGGGGCA‑GCACATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCt                                                                                    <  1:71289/148‑1 (MQ=255)
                                                             cACCGCCAAGGCGAACACGCCGATGGGCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTg                                                                                   >  2:159938/1‑149 (MQ=255)
                                                              aCCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGt                                                                                  >  1:110030/1‑149 (MQ=255)
                                                               ccGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTc                                                                                 >  6:250976/1‑149 (MQ=255)
                                                                cGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCt                                                                                >  7:196282/1‑149 (MQ=255)
                                                                  ccAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCATGCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGCGTAGGCTGTCtt                                                                               <  1:91679/149‑1 (MQ=255)
                                                                        cGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑AAGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTgc                                                                        <  1:97523/149‑1 (MQ=255)
                                                                         gAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTgcg                                                                       <  3:188212/149‑1 (MQ=255)
                                                                                 cGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCCGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCcg                                                               <  6:383986/149‑1 (MQ=255)
                                                                                      gCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATcggcg                                                                                                                                         <  6:63831/73‑1 (MQ=255)
                                                                                      gCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATcggcg                                                                                                                                         >  5:63831/1‑73 (MQ=255)
                                                                                      gCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGATTTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGt                                                           <  1:102491/149‑1 (MQ=255)
                                                                                         aGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAAt                                                       >  6:270525/1‑149 (MQ=255)
                                                                                             acacGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATcgcg                                                   <  5:250976/149‑1 (MQ=255)
                                                                                              cacGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCgg                                                  <  7:120531/149‑1 (MQ=255)
                                                                                               acGGCAGCAGCCAATGTACCGGCCTGCGGATGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGtt                                                                          >  1:29744/1‑124 (MQ=255)
                                                                                               acGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGtt                                                                          <  2:29744/124‑1 (MQ=255)
                                                                                               acGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAGTCGCGGt                                                 >  3:363285/1‑149 (MQ=255)
                                                                                                  gcagcaGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTc                                                >  4:26546/1‑147 (MQ=255)
                                                                                                        gcCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAgcgcgc                                        >  3:257769/1‑149 (MQ=255)
                                                                                                        gcCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAgcgcgc                                        >  6:301486/1‑149 (MQ=255)
                                                                                                         ccAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAgcgcgcg                                       <  1:11942/149‑1 (MQ=255)
                                                                                                         ccAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAgcgcgcg                                       >  3:229518/1‑149 (MQ=255)
                                                                                                           aaTGTACCGGCCTCCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGCTAAATCGCGGTCCAgcgcgcgcg                                     >  7:233264/1‑149 (MQ=255)
                                                                                                            aTGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAgcgcgcgcg                                     >  7:195018/1‑148 (MQ=255)
                                                                                                            aTGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGGTTCCAGCTAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCTGTAAATCGCGGTCCAgcgcgcgcgc                                    <  8:196282/149‑1 (MQ=255)
                                                                                                               tACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCAcc                                 <  1:159938/149‑1 (MQ=255)
                                                                                                                  cGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATc                              <  4:17793/149‑1 (MQ=255)
                                                                                                                  cGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATc                              <  1:71382/149‑1 (MQ=255)
                                                                                                                      cTGCGGAAGTACCGCCAC‑GCTCAGCCGCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGcc                           >  1:200884/1‑148 (MQ=255)
                                                                                                                        gCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGc                                                                                             >  7:163481/1‑80 (MQ=255)
                                                                                                                        gCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGc                                                                                             <  8:163481/80‑1 (MQ=255)
                                                                                                                        gCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCGTCGCCGa                         >  2:258341/1‑148 (MQ=255)
                                                                                                                               tACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGCCGACATGCTgg                 <  5:270525/149‑1 (MQ=255)
                                                                                                                                  cGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGCCGACATGCTGGGCa              >  1:326715/1‑149 (MQ=255)
                                                                                                                                   gCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGCCGACATGCTGGGCAt             >  7:178717/1‑149 (MQ=255)
                                                                                                                                     cAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGCCGACATGCTGGGCATGa           <  4:254272/149‑1 (MQ=255)
                                                                                                                                              ggcccGCCGATTTCGGTGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGCCGACATGCTGGGCATGAAGCCGGc    >  2:158954/3‑148 (MQ=255)
                                                                                                                                               gcccGCCGATTTCGGTGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGCCGACATGCTGGGCATGAAGCCGGCGa  <  1:158954/148‑1 (MQ=255)
                                                                                                                                               ccccACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGCCGACATGCTGGGCATGAAGCCGGCGa  <  4:257769/149‑1 (MQ=255)
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CCGACATGCTGTCTTGCCCGGTGGCGGCATCGGCCTCTACTGGCGGCTCGGTGCCGACCTGCACCGCCAAGGCGAACACGCCGATGGCCAGGAACACGGCAGCAGCCAATGTACCGGCCTGCGGAAGTACCGCCAC‑GCTCAGCCCCACCGAAATCGGCGGGCCA‑GCA‑ATGAAGGTGA‑CTTCGTCAAGAACGGTTTCCAGCGAGTAGGCTGTCTTCAGTTGCGCCTTGCCGCGGTAAATCGCGGTCCAGCGCGCGCGCACCATCGCCGACATGCTGGGCATGAAGCCGGCGA  >  NC_002947/4470562‑4470852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑