Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,521,849 T→C 8.1% intergenic (‑112/‑303) icd ← / → idh NADP(+)‑specific isocitrate dehydrogenase/isocitrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,521,8490TC 8.1% 138.9 / 2.3 49intergenic (‑112/‑303)icd/idhNADP(+)‑specific isocitrate dehydrogenase/isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base C (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GTCGGTCGGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGT  >  NC_002947/4521705‑4521997
                                                                                                                                                |                                                                                                                                                       
gtcggtcgGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGCCttt                                                                                                                                                     >  3:92453/1‑149 (MQ=255)
gtcggtcgGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGCCttt                                                                                                                                                     >  3:87310/1‑149 (MQ=255)
gtcggtcgGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGATGTCGTGGGTGGTTTGGACATCGTCCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGTCATGCTACAACCCGCCGCATTGCTGCTTAGTCttt                                                                                                                                                     <  2:276933/149‑1 (MQ=255)
gtcgggcgGAACCTTGAGTTTCTGGTATCCCATGCTTGCACTACTCCGCTGGCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCttt                                                                                                                                                     <  6:229470/149‑1 (MQ=255)
    gtcggAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGt                                                                                                                                                         >  4:82211/1‑141 (MQ=255)
    gtcggAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGt                                                                                                                                                         <  3:82211/141‑1 (MQ=255)
    gtcggAACCTTGATGTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCACATTGCTGCTTAGTCTTTGGTc                                                                                                                                                 <  2:43987/149‑1 (MQ=255)
        gAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTta                                                                                                                                              >  7:161934/1‑148 (MQ=255)
            cTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAAt                                                                                                                                         >  8:13063/1‑149 (MQ=255)
                   ttCTGGTACCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCAGGGCGGCTTAGTCGGTGGGCTTGTAAATGGGCCgc                                                                                                                                  >  4:300881/1‑148 (MQ=255)
                         taacccATGCTTGCACTACTCCGCTGTCTGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCAc                                                                                                                            <  7:338353/146‑1 (MQ=255)
                             ccATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTgg                                                                                                                        >  1:232994/1‑149 (MQ=255)
                                     gCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTg                                                                                                                                                    >  5:234651/1‑113 (MQ=255)
                                     gCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTg                                                                                                                                                    <  6:234651/113‑1 (MQ=255)
                                      cACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTg                                                                                                                <  5:202795/148‑1 (MQ=255)
                                       actactCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGAt                                                                                                              <  8:161934/149‑1 (MQ=255)
                                            tCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGc‑‑‑c                                                                                                      >  2:212500/1‑149 (MQ=255)
                                            tCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGc‑‑‑c                                                                                                      >  5:116211/1‑149 (MQ=255)
                                               gCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCa                                                                                                    >  5:161954/1‑148 (MQ=255)
                                                  gTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATg                                                                                                >  1:201879/1‑149 (MQ=255)
                                                   tCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGCCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAAt                                                                                                 <  4:92453/147‑1 (MQ=255)
                                                   tCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGCCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAAt                                                                                                 <  4:87310/147‑1 (MQ=255)
                                                      ggTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGttt                                                                                            >  6:113384/1‑149 (MQ=255)
                                                       gtgtGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTg                                                                                           <  2:82586/149‑1 (MQ=255)
                                                          tgGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGta                                                                                        >  4:63454/1‑149 (MQ=255)
                                                                    aTCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAAt                                                                                                 <  3:290258/130‑1 (MQ=255)
                                                                    aTCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAAt                                                                                                 >  4:290258/1‑130 (MQ=255)
                                                                       gTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGa                                                                           <  2:201879/149‑1 (MQ=255)
                                                                        tGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGAc                                                                          >  4:305637/1‑149 (MQ=255)
                                                                        tGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGAc                                                                          >  3:168762/1‑149 (MQ=255)
                                                                            aTCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGaa                                                                      <  4:154719/149‑1 (MQ=255)
                                                                               cACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAgg                                                                    <  6:161954/148‑1 (MQ=255)
                                                                                aCTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGata                                                                                                                                <  6:215893/90‑1 (MQ=255)
                                                                                         aaaCCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGc                                                         <  3:305637/149‑1 (MQ=255)
                                                                                             cacaTCTGCCGACCCGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCCTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAAt                                                     >  1:170905/1‑149 (MQ=255)
                                                                                              acaTCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATc                                                    >  7:295445/1‑149 (MQ=255)
                                                                                              acaTCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATc                                                    <  7:13063/149‑1 (MQ=255)
                                                                                                    gCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAgg                                               >  3:255764/1‑148 (MQ=255)
                                                                                                       gACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGaaaa                                           >  5:276453/1‑149 (MQ=255)
                                                                                                        aCCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAAt                                          <  5:113384/149‑1 (MQ=255)
                                                                                                          cTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGCGTCATCGGGGCGAATCCCAGGAAAATGc                                        <  1:212500/149‑1 (MQ=255)
                                                                                                            gCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCa                                                 <  8:172107/138‑1 (MQ=255)
                                                                                                            gCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCa                                                 >  7:172107/1‑138 (MQ=255)
                                                                                                             cAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCg                                       <  3:85803/147‑1 (MQ=255)
                                                                                                             cAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGa                                     >  5:18615/1‑149 (MQ=255)
                                                                                                               aGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCCTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGAcc                                   <  2:170905/149‑1 (MQ=255)
                                                                                                                          aaCCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCTGGGGAAATGGTTTGTTATACTCCGCCGCGACCGAAGGGTAATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAgg                           >  1:116650/1‑149 (MQ=255)
                                                                                                                                 cgcATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATgg                 <  4:255764/149‑1 (MQ=255)
                                                                                                                                  gcATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGc                <  4:168762/149‑1 (MQ=255)
                                                                                                                                        ctgctTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATa            >  5:221331/1‑147 (MQ=255)
                                                                                                                                           ctTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGcc       >  5:185156/1‑149 (MQ=255)
                                                                                                                                                tCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGgt  <  6:276453/149‑1 (MQ=255)
                                                                                                                                                tCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGgt  <  8:295445/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                       
GTCGGTCGGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGC‑‑‑CCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGT  >  NC_002947/4521705‑4521997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑