BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	345,714	G→C	5.1%	A711G (GCC→GGC)	PP_0287 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	345,714	0	G	C	5.1%	310.5 / 5.1	97	A711G (GCC→GGC)	PP_0287	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (43/49); new base C (3/2); total (46/51)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 103 total aligned reads displayed.

AGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTGGTCGC  >  NC_002947/345566‑345858

aGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTgg                                                                                                                                                  <  2:215731/149‑1 (MQ=255)

aGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTgg                                                                                                                                                  >  2:376226/1‑149 (MQ=255)

 gTCCAGCACTGCGCCGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCATCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggc                                                                                                                                                 <  5:138762/149‑1 (MQ=255)

    cAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggcgg                                                                                                                                               <  8:212436/148‑1 (MQ=255)

     aGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCa                                                                                                                                             <  4:310651/149‑1 (MQ=255)

      gCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCaa                                                                                                                                            >  7:109470/1‑149 (MQ=255)

        aCTGCGCTGGAGAACTGCTCGGGGCTCATCGGTGGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGCCAAGCTGGCCGGCAAcc                                                                                                                                          <  5:164029/149‑1 (MQ=255)

            cgcTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTg                                                                                                                                       <  1:158994/148‑1 (MQ=255)

             gcTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAACTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGCCAAGCTGGCCGGCAAccagacg                                                                                                                                     >  2:160584/1‑144 (MQ=255)

               tGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGcc                                                                                                                                     >  2:368735/1‑147 (MQ=255)

               tGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCAGCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGcc                                                                                                                                     >  6:377960/1‑147 (MQ=255)

                      cTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGtt                                                                                                                            <  4:323848/149‑1 (MQ=255)

                       tGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTc                                                                                                                           >  2:63137/1‑149 (MQ=255)

                          tCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggc                                                                                                                                                 >  6:345348/1‑124 (MQ=255)

                          tCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggc                                                                                                                                                 <  5:345348/124‑1 (MQ=255)

                            ggggCTCATCTGTTGAACAGCACATTGAGCGCG‑TCATTCAGTTCCGGGCTCACTTTGTCTCAAAGTTTTTCGTCAAGTTTTTAATCGATCTTGTCTTTCAGGCGTTTGCATGCACGATTTGCGGCAACATTGCCCAGGACGTTCTgg                                                                                                                        >  8:167216/1‑147 (MQ=255)

                            ggggCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTgg                                                                                                                        <  4:42372/147‑1 (MQ=255)

                              ggCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTc                                                                                                                      >  8:12346/1‑147 (MQ=255)

                              ggCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTc                                                                                                                      >  5:93412/1‑147 (MQ=255)

                              ggCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGa                                                                                                                    <  6:338818/149‑1 (MQ=255)

                               gCTCATCGGTTGAACAGCCCCTTGAGCGCGATCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTc                                                                                                                      >  1:33712/1‑147 (MQ=255)

                                 tCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAggc                                                                                                                 >  3:120065/1‑149 (MQ=255)

                                   aTCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAggcgg                                                                                                               >  3:127278/1‑149 (MQ=255)

                                    tCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAggcggc                                                                                                              <  8:109470/149‑1 (MQ=255)

                                        ttGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGc                                                                                                          >  4:55679/1‑149 (MQ=255)

                                           aaCAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCtt                                                                                                       >  4:126973/1‑149 (MQ=255)

                                            aCAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTAGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTAGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCttt                                                                                                      >  1:96703/1‑149 (MQ=255)

                                              aGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTgc                                                                                                    >  3:19752/1‑149 (MQ=255)

                                                ccccTTGCGCGCG‑TCTTTCAGTTCCGTGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTgcgc                                                                                                  <  7:270279/149‑1 (MQ=255)

                                                 cccTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGcc                                                                                                 <  4:166703/149‑1 (MQ=255)

                                                 cccTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGcc                                                                                                 >  3:166703/1‑149 (MQ=255)

                                                  ccTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGcc                                                                                                 >  1:165595/1‑148 (MQ=255)

                                                         cgcg‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCa                                                                                         >  4:5544/1‑149 (MQ=255)

                                                          gcg‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAg                                                                                        <  1:376226/149‑1 (MQ=255)

                                                          gcg‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTCGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAg                                                                                        <  6:59302/149‑1 (MQ=255)

                                                            g‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTgc                                                                                                    >  5:28940/1‑135 (MQ=255)

                                                            g‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTgc                                                                                                    <  6:28940/135‑1 (MQ=255)

                                                            g‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCg                                                                                      >  7:141431/1‑149 (MQ=255)

                                                                 ttCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGAc                                                                                   >  7:309056/1‑148 (MQ=255)

                                                                  tCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGAc                                                                                   <  3:126973/147‑1 (MQ=255)

                                                                   cAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCa                                                                                 <  3:5544/148‑1 (MQ=255)

                                                                    aGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACg                                                                               <  6:306463/149‑1 (MQ=255)

                                                                    aGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACg                                                                               <  2:33712/149‑1 (MQ=255)

                                                                     gTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTGCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACgg                                                                              <  3:132151/149‑1 (MQ=255)

                                                                      ttCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACgg                                                                              >  5:271688/1‑148 (MQ=255)

                                                                        ccGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCa                                                                            <  2:372974/148‑1 (MQ=255)

                                                                          gggCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAgcg                                                                         <  1:368735/149‑1 (MQ=255)

                                                                                aCTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAgggg                                                                   <  6:271688/149‑1 (MQ=255)

                                                                                aCTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAgggg                                                                   <  5:59569/149‑1 (MQ=255)

                                                                                aCTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAgggg                                                                   <  1:63137/149‑1 (MQ=255)

                                                                                      tctcCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTc                                                             <  6:133969/149‑1 (MQ=255)

                                                                                        tcCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCaa                                                           >  3:353458/1‑149 (MQ=255)

                                                                                         ccAAGTTTTTTGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCTAGCGGACCACGGCAGCGCAGGGGGACTTCaa                                                           >  2:162813/1‑148 (MQ=255)

                                                                                         ccAAGTTTTTTGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCTAGCGGACCACGGCAGCGCAGGGGGACTTCAac                                                          >  2:157223/1‑149 (MQ=255)

                                                                                          cAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCaa                                                           <  4:120065/147‑1 (MQ=255)

                                                                                          cAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAaca                                                         >  5:364668/1‑149 (MQ=255)

                                                                                          cAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGACTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCaa                                                           <  1:366167/147‑1 (MQ=255)

                                                                                            aGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACAcc                                                       <  7:12346/149‑1 (MQ=255)

                                                                                             gTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACAccc                                                      <  4:127278/149‑1 (MQ=255)

                                                                                                   cGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATa                                                 <  4:19752/148‑1 (MQ=255)

                                                                                                    gTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAc                                               <  8:141431/149‑1 (MQ=255)

                                                                                                    gTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAc                                               >  3:58157/1‑149 (MQ=255)

                                                                                                     tCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGAGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcg                                              >  6:222862/1‑149 (MQ=255)

                                                                                                     tCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCAAGATTGGCGGCAACCTTGCCCAGGCCCTTCTGTTCGAGGCGGCAGGCCTTTGAGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATaaac                                              >  1:322428/1‑147 (MQ=255)

                                                                                                       aaGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgtt                                            >  3:301880/1‑149 (MQ=255)

                                                                                                       aaGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgt                                             <  6:46340/148‑1 (MQ=255)

                                                                                                        aGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttc                                           >  1:371186/1‑149 (MQ=255)

                                                                                                         gTTTTTCATCGATCTTGTCTTTCAGGCGCTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcg                                          >  1:211591/1‑149 (MQ=255)

                                                                                                          tttttCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcgt                                         <  2:371186/149‑1 (MQ=255)

                                                                                                                aTCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGTAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCt                                   >  3:5060/1‑149 (MQ=255)

                                                                                                                aTCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGAcc                                    <  4:353458/148‑1 (MQ=255)

                                                                                                                aTCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCt                                   >  7:271862/1‑149 (MQ=255)

                                                                                                                 tCGATATTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTGCAGCGGACCACGGCAGCGCAGGGGGACTTCAACCCCCACATAACGTTCGTTGAcccg                                  >  6:351037/1‑147 (MQ=255)

                                                                                                                     tCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTCGCGGCAACGTTGCCCAGGCCGTTCTGCTCTAGGCGGCAGGCCTTAGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGc                                <  7:167216/147‑1 (MQ=255)

                                                                                                                       ttGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGc                            >  7:297078/1‑149 (MQ=255)

                                                                                                                       ttGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGc                            <  8:271862/149‑1 (MQ=255)

                                                                                                                          tCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGc                                                                             >  7:86137/1‑97 (MQ=255)

                                                                                                                          tCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCgg                         <  4:301880/149‑1 (MQ=255)

                                                                                                                          tCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCgg                         >  2:294123/1‑149 (MQ=255)

                                                                                                                              tCAGGCGGTTGCTGGCCAGCTTGGCGGCAACCTTGCCCTGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTcc                     >  6:94687/1‑149 (MQ=255)

                                                                                                                              tCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTc                      <  6:364668/148‑1 (MQ=255)

                                                                                                                               cAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTcc                     <  8:297078/148‑1 (MQ=255)

                                                                                                                                aGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACTCCCACATAACGTTCGTTGACCCGGCCTGCCAGGTcaac                   <  5:351037/149‑4 (MQ=255)

                                                                                                                                aGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCCGGCCTGCCAGGTcaa                    <  3:55679/148‑3 (MQ=255)

                                                                                                                                 ggCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCgg                   >  2:323978/1‑148 (MQ=255)

                                                                                                                                  aaggTTGCCGCCAAGCTGGCCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAaca                                                         >  7:335703/3‑109 (MQ=39)

                                                                                                                                  aaggTTGCCGCCAAGCTGGCCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAaca                                                         <  8:335703/107‑1 (MQ=39)

                                                                                                                                   cGGTTGCTGGCCAGCTTGGCGGCAACCTTGCCCTGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCAt                <  5:94687/149‑1 (MQ=255)

                                                                                                                                   cGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGTAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCAt                <  4:5060/149‑1 (MQ=255)

                                                                                                                                   cGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCAt                <  4:356179/149‑1 (MQ=255)

                                                                                                                                    ggTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCAGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATg               <  5:323447/149‑1 (MQ=255)

                                                                                                                                    ggTTGCCGGCCAGCTTGGCGGCAACCGTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGACTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATg               <  6:93412/149‑1 (MQ=255)

                                                                                                                                        gCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCAc           >  8:290125/1‑149 (MQ=255)

                                                                                                                                         ccGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCAc           <  7:290125/148‑1 (MQ=255)

                                                                                                                                          cGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGc         >  2:284255/1‑149 (MQ=255)

                                                                                                                                            gCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTg       <  2:165595/149‑1 (MQ=255)

                                                                                                                                            gCCAGCTTGGCGGCAACCTTGCCCAGGCCGGTCTGGGCGAGGCGGCAGGCCGTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTg       <  4:297603/149‑1 (MQ=255)

                                                                                                                                            ccaaGCTGGCCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTg       >  1:232677/4‑149 (MQ=255)

                                                                                                                                             ccAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTgg      <  8:309056/149‑1 (MQ=255)

                                                                                                                                                 attGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCACCGGACCACGGCAGCGCAGGGGGCGTTCAACACCCACATAACGTTCGTTGCCCTGGCCGGCCGGGTCCGGCATGGCACGCTGGTCGc  >  5:265617/2‑149 (MQ=255)

AGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCG‑TCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTGGTCGC  >  NC_002947/345566‑345858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`