| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,610,296 | C→G | 7.3% | I261M (ATC→ATG) | PP_4081 → | type IV/VI secretion system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,610,296 | 0 | C | G | 7.3% | 170.0 / 3.9 | 55 | I261M (ATC→ATG) | PP_4081 | type IV/VI secretion system protein |
| Reads supporting (aligned to +/- strand): ref base C (24/27); new base G (2/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
CACTGATTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCG > NC_002947/4610148‑4610443 | cACTGATTCACCAACAGCACGCGATCATCCGCCCACTCCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCCTGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCcgccatc < 7:129795/149‑1 (MQ=18) cTGATTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCcgccatcgc < 2:82310/149‑1 (MQ=255) tCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATgg > 2:271931/1‑149 (MQ=255) cACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATgg < 7:111406/148‑1 (MQ=255) aCCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGCCCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCg > 1:107775/1‑149 (MQ=255) aCCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCg > 2:75457/1‑149 (MQ=255) aCCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCg < 2:225532/149‑1 (MQ=255) aaCTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTg > 4:107211/1‑149 (MQ=255) aaCTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTg < 1:8686/149‑1 (MQ=255) aCTGCACGGGAGCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCACCGCCATGGCGATGGCggaga < 5:47678/149‑4 (MQ=18) gggATCATCCGCGAACTGCGCGGCCCCACCCCCGCAGAAGGCTGCGCCCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCTTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACgg < 7:250108/149‑1 (MQ=255) atcCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTa > 5:87600/1‑149 (MQ=255) tcCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGTGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTa > 1:118665/1‑148 (MQ=255) tcCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTAc < 6:87600/149‑1 (MQ=255) tcCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTAc < 1:39062/149‑1 (MQ=255) cgcgAACTGCGCGGCCCCACCCCCGAAGCAGTCTGCGACCCCTACACCAACGTCCCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCAGCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAg > 8:8021/1‑149 (MQ=255) cgGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGc < 2:3949/149‑1 (MQ=255) ccACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCt > 3:154265/1‑148 (MQ=255) cACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGa < 6:382097/149‑1 (MQ=255) cACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCATGCACCTGa > 3:248199/1‑149 (MQ=255) cccGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATcac < 3:107211/149‑1 (MQ=255) ccGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATcac > 2:366891/1‑148 (MQ=255) gaagTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGa < 5:73952/149‑1 (MQ=255) aagTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAg < 1:75457/149‑1 (MQ=255) gCGACCCCTACACCAACGTCGCCACCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTAATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTg > 6:83175/1‑149 (MQ=255) cGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTg < 1:366891/148‑1 (MQ=255) cccTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTc > 6:350782/1‑149 (MQ=255) ccTACACCAACGTCGCCCCAAGAAACTTCCGCATGAGC‑CGGATATCTACGTGGTGGAGCCCGTTGGTCATCTCCGACATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCATGCACATGATCACAACCGAGGTGCTGGAGTCg > 3:214963/1‑149 (MQ=17) cacCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCt < 5:240289/147‑1 (MQ=255) ccAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAAc < 1:271931/149‑1 (MQ=255) cAACGTCGCCCCCAGAAACTTCCGCATGAGCGCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAAc > 1:229209/1‑149 (MQ=255) cAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACg > 6:143720/1‑149 (MQ=255) cccAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca < 2:322762/149‑1 (MQ=255) cccAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca < 4:259427/149‑1 (MQ=255) aGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgcagca < 3:297555/149‑1 (MQ=255) aCTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAg < 4:179469/85‑1 (MQ=255) aCTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAg > 3:179469/1‑85 (MQ=255) gC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTca < 2:118665/149‑1 (MQ=255) c‑cGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTcac < 5:143720/149‑1 (MQ=255) gCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACt < 4:154265/149‑1 (MQ=255) ggggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTc < 1:43235/147‑1 (MQ=39) tgtaGCCCGTTGGTCATCTCCGCGATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTTGAGTCGCTGAACGGCATTTTGAAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCa < 4:214963/146‑1 (MQ=255) tggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCa < 2:229209/149‑1 (MQ=39) gCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACg > 8:199527/1‑148 (MQ=39) ccGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAAt > 5:48066/1‑149 (MQ=39) cGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAAtt > 5:284483/1‑149 (MQ=38) gTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTc > 8:202576/1‑149 (MQ=38) gTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGa < 1:373426/149‑1 (MQ=37) gTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGa < 2:107775/149‑1 (MQ=37) tCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAg > 3:153563/1‑149 (MQ=35) gccaccGCCATGGCGATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAAc > 6:375181/5‑136 (MQ=12) gccaccGCCATGGCGATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAAc < 5:375181/132‑1 (MQ=12) aTCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTTTCACAGCACCACTGAGTTCAACGAATTCGCGa > 1:160696/1‑146 (MQ=21) aTCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGa < 2:160696/146‑1 (MQ=34) tctcCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCAGTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGtt > 7:78566/1‑149 (MQ=18) caccGCCATGGCGATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGttt > 2:173133/3‑149 (MQ=12) tcCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGtttt > 2:38717/1‑149 (MQ=32) ggccaccgccatGGCGATGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTg > 5:281077/2‑149 (MQ=17) catcgccatGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCgt > 2:151031/1‑105 (MQ=32) catcgccatGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCgt < 1:151031/105‑1 (MQ=32) atcgccatGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTc > 4:370025/1‑149 (MQ=32) tcgccatGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCg > 8:238420/1‑149 (MQ=32) | CACTGATTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGC‑CGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCG > NC_002947/4610148‑4610443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |