Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,691,599 A→G 5.3% D88G (GAC→GGC)  PP_4152 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,691,5990AG 5.3% 226.9 / 2.0 76D88G (GAC→GGC) PP_4152hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/35);  new base G (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

AGCATTCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGGACGACTTCATCGCT  >  NC_002947/4691454‑4691747
                                                                                                                                                 |                                                                                                                                                    
aGCATTCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACtt                                                                                                                                                   <  6:332007/149‑1 (MQ=255)
 gCATTCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTg                                                                                                                                                  >  1:151642/1‑149 (MQ=255)
    ttCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCa                                                                                                                                               <  4:101886/149‑1 (MQ=255)
    ttCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCa                                                                                                                                               <  2:95255/149‑1 (MQ=255)
       aTGACGAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGa                                                                                                                                             >  2:337464/1‑148 (MQ=255)
           cGAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAggg                                                                                                                                        <  4:139972/149‑1 (MQ=255)
            gAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGga                                                                                                                                       >  4:268622/1‑149 (MQ=255)
            gAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGga                                                                                                                                       >  6:159865/1‑149 (MQ=255)
                    gcgcAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGa                                                                                                                                >  1:217321/1‑148 (MQ=255)
                      gcAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACAt                                                                                                                             >  5:190302/1‑149 (MQ=255)
                       cAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATg                                                                                                                            <  2:151642/149‑1 (MQ=255)
                              cTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTtct                                                                                                                        <  7:50528/146‑1 (MQ=255)
                              cTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCg                                                                                                                      <  5:43524/148‑1 (MQ=255)
                                 cTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGAc                                                                                                                               >  5:22856/1‑136 (MQ=255)
                                 cTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGAc                                                                                                                               <  6:22856/136‑1 (MQ=255)
                                  tCAGCCGACCAGACCTCACGCCTGAACATTTGGCACGCACGTCCTCCCTGATGGCTGCTGCGGTGCCTGAGGAACTCCTCACTGACTAGGATTCAGGGCTCGAAGGCCTGGACGTGCCAGAGAGGGATGCTCGACAAGTTCTCGCAGCt                                                                                                                 >  8:106588/1‑149 (MQ=255)
                                  tCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCt                                                                                                                 >  3:244220/1‑149 (MQ=255)
                                  tCAACCGACCTGACCTCACGCCTGAACATATGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCt                                                                                                                 >  1:27832/1‑149 (MQ=255)
                                    aaCCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTg                                                                                                                >  7:260408/1‑148 (MQ=255)
                                     aCCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGc                                                                                                               >  1:362501/1‑148 (MQ=255)
                                          cctgacctCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCaa                                                                                                         <  8:203251/149‑1 (MQ=255)
                                                ctCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCaa                                                                                                   >  3:71982/1‑149 (MQ=255)
                                                   aCGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAAc                                                                                                  <  1:185454/147‑1 (MQ=255)
                                                     gCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGGCTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCtt                                                                                              >  5:341209/1‑149 (MQ=255)
                                                     gCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATc                                                                                                                                  <  7:115786/113‑1 (MQ=255)
                                                     gCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATc                                                                                                                                  >  8:115786/1‑113 (MQ=255)
                                                     gCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCtt                                                                                              >  4:308851/1‑149 (MQ=255)
                                                      ccTGAACATTTGGATCGCACGGCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTc                                                                                             <  2:46674/149‑1 (MQ=255)
                                                       cTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCg                                                                                            >  4:242745/1‑149 (MQ=255)
                                                         gAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCAACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCgg                                                                                           >  1:313035/1‑148 (MQ=255)
                                                             aTTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGGCTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATc                                                                                      >  7:25796/1‑149 (MQ=255)
                                                                  gATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCACCGCTACGGTCATCGTCAc                                                                                 >  8:354190/1‑149 (MQ=255)
                                                                       cACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTc                                                                             >  6:103248/1‑148 (MQ=255)
                                                                       cACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTc                                                                             >  1:135026/1‑148 (MQ=255)
                                                                        aCGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCg                                                                                            <  7:18910/132‑1 (MQ=255)
                                                                        aCGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCg                                                                                            >  8:18910/1‑132 (MQ=255)
                                                                        aCGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCaa                                                                           <  6:190302/149‑1 (MQ=255)
                                                                          gTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCTAGTGCAACGCTTCGGTCATCGTCACCTTCAAtt                                                                         >  6:376907/1‑149 (MQ=255)
                                                                            cATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGCCACCTTCAAttt                                                                        >  1:166496/1‑148 (MQ=255)
                                                                             aTCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGa                                                                      <  3:268622/149‑1 (MQ=255)
                                                                             aTCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTAACCTTCAATTTg                                                                       >  6:315164/1‑148 (MQ=255)
                                                                              tCGCTGCTGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGaa                                                                     <  6:198699/149‑1 (MQ=255)
                                                                              tCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGaa                                                                     <  2:217321/149‑1 (MQ=255)
                                                                                 cTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGGCTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGa                                                                  <  8:25796/149‑1 (MQ=255)
                                                                                           cagctgTGCCTGATGCACTCGTCACTGTCTACGTTTCATTGGTCGTAGGGGTGGTCTTGCCAGTTAGGGATGACCGACATGTTCTGGCAGGTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGTTGGATTTTTCCAAg                                                        <  7:106588/144‑1 (MQ=255)
                                                                                              cGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCg                                                     <  4:71982/149‑1 (MQ=255)
                                                                                                 tGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGtt                                                  <  5:103248/149‑1 (MQ=255)
                                                                                                 tGCCTGAAGCACTCGGCACTGACTACGATTCACGGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCACTCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCggat                                                  <  6:279972/149‑3 (MQ=255)
                                                                                                   ccTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGGCTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTc                                                 <  6:341209/148‑1 (MQ=255)
                                                                                                      gATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTgg                                             >  2:327511/1‑149 (MQ=255)
                                                                                                      gATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTgg                                             >  2:77052/1‑149 (MQ=255)
                                                                                                       aTGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTgg                                             <  2:135026/148‑1 (MQ=255)
                                                                                                       aTGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGt                                            <  2:313035/149‑1 (MQ=255)
                                                                                                         gCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGc                                          >  6:383824/1‑149 (MQ=255)
                                                                                                          cACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTc                                                            >  7:173525/1‑130 (MQ=255)
                                                                                                          cACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTc                                                            <  6:20855/130‑1 (MQ=255)
                                                                                                          cACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTc                                                            <  8:173525/130‑1 (MQ=255)
                                                                                                          cACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTc                                                            >  5:20855/1‑130 (MQ=255)
                                                                                                             tCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTc                                                 <  3:148682/138‑1 (MQ=255)
                                                                                                             tCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTc                                                 >  4:148682/1‑138 (MQ=255)
                                                                                                               gTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCATTTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGa                                    >  4:248351/1‑149 (MQ=255)
                                                                                                               gTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCg                                     <  2:27832/148‑1 (MQ=255)
                                                                                                               gTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGCCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTc                                      <  2:166496/147‑1 (MQ=255)
                                                                                                                   cTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATAtt                                <  5:383824/149‑1 (MQ=255)
                                                                                                                    tGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATAtt                                >  8:202867/1‑148 (MQ=255)
                                                                                                                        tACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGt                                                                                                       >  1:89942/1‑73 (MQ=255)
                                                                                                                        tACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGt                                                                                                       <  2:89942/73‑1 (MQ=255)
                                                                                                                        tACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGt                                            <  3:136362/132‑1 (MQ=255)
                                                                                                                        tACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGt                                            >  4:136362/1‑132 (MQ=255)
                                                                                                                          cGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCTGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAAcc                           <  7:202867/147‑1 (MQ=255)
                                                                                                                             ttCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGc                      <  1:337464/149‑1 (MQ=255)
                                                                                                                                    gTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGga               >  2:95/1‑149 (MQ=255)
                                                                                                                                     tCGAACGGCTGGACTTGCCAGATAGGGATGAGCGACATGCTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGgac              <  4:244220/149‑1 (MQ=255)
                                                                                                                                        atgcgCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCACTCAAGTGCAACGCTTCGGTCCTCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGgacgac           <  2:255913/145‑1 (MQ=255)
                                                                                                                                        aaGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGgacgac           <  8:260408/149‑1 (MQ=255)
                                                                                                                                             cTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGGACGACTTCAt      >  2:22409/1‑149 (MQ=255)
                                                                                                                                                 aCTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGGACGACTTCATCGCt  <  3:308851/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGCATTCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGACCTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTACGATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTCGGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGGACGACTTCATCGCT  >  NC_002947/4691454‑4691747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑