Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,694,425 C→G 6.3% P767R (CCC→CGC)  PP_4154 → class 3 aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,694,4250CG 6.3% 256.5 / 5.5 79P767R (CCC→CGC) PP_4154class 3 aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (33/41);  new base G (2/3);  total (35/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

CCGCGGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATG  >  NC_002947/4694279‑4694573
                                                                                                                                                  |                                                                                                                                                    
ccGCGGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGccc                                                                                                                                                     <  4:149540/148‑1 (MQ=255)
 cgcgGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCg                                                                                                                                                    >  5:317034/1‑148 (MQ=255)
  gcgGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGCCGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCg                                                                                                                                                    >  2:370784/1‑147 (MQ=255)
   cgGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAg                                                                                                                                                 <  2:267528/149‑1 (MQ=255)
   cgGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAg                                                                                                                                                 >  2:228033/1‑149 (MQ=255)
   cgGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAg                                                                                                                                                 <  6:128707/149‑1 (MQ=255)
     gCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGc                                                                                                                                               <  5:353148/149‑1 (MQ=255)
       cGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTa                                                                                                                                             >  5:285915/1‑149 (MQ=255)
        gCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTAc                                                                                                                                            <  5:52926/149‑1 (MQ=255)
                  ggCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAg                                                                                                                                  <  1:9495/149‑1 (MQ=255)
                  ggCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAg                                                                                                                                  >  6:185766/1‑149 (MQ=255)
                   gCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGc                                                                                                                                 >  6:67664/1‑149 (MQ=255)
                    ccGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAGcg                                                                                                                                <  7:108239/149‑2 (MQ=255)
                     cGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAGcga                                                                                                                               <  5:208867/149‑3 (MQ=255)
                     cGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGcc                                                                                                                                >  7:150578/1‑148 (MQ=255)
                              cgccgACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAgg                                                                                                                      >  8:62322/1‑149 (MQ=255)
                                   aCTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                                 <  5:289845/149‑1 (MQ=255)
                                   aCTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                                 <  3:68869/149‑1 (MQ=255)
                                   aCTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                                 <  6:317034/149‑1 (MQ=255)
                                     tACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGTAGCCTgcggg                                                                                                                                     >  1:282693/1‑125 (MQ=255)
                                     tACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGTAGCCTgcggg                                                                                                                                     <  2:282693/127‑3 (MQ=255)
                                       ccTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgcg                                                                                                                >  1:79057/1‑146 (MQ=255)
                                       ccTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgcg                                                                                                                <  2:79057/146‑1 (MQ=255)
                                          gCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCcgcg                                                                                                          >  3:70791/1‑149 (MQ=255)
                                             aGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCgcggcg                                                                                                       >  6:146970/1‑149 (MQ=255)
                                             aGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCgcggcg                                                                                                       <  1:228033/149‑1 (MQ=255)
                                               aCGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCggg                                                                                                     <  3:269212/149‑1 (MQ=255)
                                               aCGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCggg                                                                                                     <  5:13879/149‑1 (MQ=255)
                                                cGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCgggg                                                                                                    <  7:304582/149‑1 (MQ=255)
                                                 gTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                   >  6:106717/1‑149 (MQ=255)
                                                 gTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                   >  4:195292/1‑149 (MQ=255)
                                                    gACCCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                                <  6:357677/149‑1 (MQ=255)
                                                    gACACCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                                >  5:357677/1‑149 (MQ=255)
                                                     aCGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                                >  6:124598/1‑148 (MQ=255)
                                                         cAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCg                                                                                           >  2:102633/1‑149 (MQ=255)
                                                            gctggctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcg                                                                                        <  7:62322/149‑1 (MQ=255)
                                                             ctggctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcga                                                                                       <  4:277761/149‑1 (MQ=255)
                                                                gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgacg                                                                                     >  2:257426/1‑148 (MQ=255)
                                                                gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgacg                                                                                     >  2:220026/1‑148 (MQ=255)
                                                                gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgac                                                                                      <  1:346283/147‑1 (MQ=255)
                                                                gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCAGCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgac                                                                                      <  6:285915/147‑1 (MQ=255)
                                                                  tgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAg                                                                                  >  6:293343/1‑149 (MQ=255)
                                                                  tgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAg                                                                                  >  7:166397/1‑149 (MQ=255)
                                                                      cTCGAGGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGATCCGGTGTACGGCATTGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCa                                                                              <  4:51968/149‑1 (MQ=255)
                                                                        cGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                   <  8:228249/126‑1 (MQ=255)
                                                                        cGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                   >  7:228249/1‑126 (MQ=255)
                                                                         gACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAgg                                                                            <  4:79726/148‑1 (MQ=255)
                                                                         gACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGt                                                                           <  6:76239/149‑1 (MQ=255)
                                                                          aCGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAgg                                                                            >  2:175127/1‑147 (MQ=255)
                                                                                   gCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGgc                                                                 <  4:4247/149‑1 (MQ=255)
                                                                                      gTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGgccgc                                                              <  5:106717/149‑1 (MQ=255)
                                                                                          gCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTgg                                                          <  5:67664/149‑1 (MQ=255)
                                                                                           cTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTggg                                                         <  8:150578/149‑1 (MQ=255)
                                                                                           cTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTggg                                                         <  5:124598/149‑1 (MQ=255)
                                                                                            tGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGc                                                        >  2:66494/1‑149 (MQ=255)
                                                                                              gCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGc                                                        >  1:227576/1‑147 (MQ=255)
                                                                                              gCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGa                                                      <  8:305134/149‑1 (MQ=255)
                                                                                               cGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAg                                                     <  1:257426/149‑1 (MQ=255)
                                                                                                        tcTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTgg                                            >  5:289878/1‑149 (MQ=255)
                                                                                                           gCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTCCTTCTGGGGc                                         >  8:152219/1‑149 (MQ=255)
                                                                                                            cGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTggg                                           <  2:227576/146‑1 (MQ=255)
                                                                                                            cGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCt                                        >  1:60256/1‑149 (MQ=255)
                                                                                                              aaCCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTc                                      <  1:175127/149‑1 (MQ=255)
                                                                                                                 cGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAg                                   <  1:66494/149‑1 (MQ=255)
                                                                                                                  ggTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAgg                                  <  4:70791/149‑1 (MQ=255)
                                                                                                                          gCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCATATGCCAACGTCCGCACCCGCGGCGGGGTGTGCATCGACGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGc                          >  1:94863/1‑149 (MQ=255)
                                                                                                                             aTGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgtg                       <  1:220026/149‑1 (MQ=255)
                                                                                                                                  ggctgTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCTCCGACGAAGTGCAgg                                                                            <  3:291146/91‑1 (MQ=255)
                                                                                                                                  ggcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGcc                  <  8:125860/149‑1 (MQ=255)
                                                                                                                                  ggcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGcc                  >  1:178355/1‑149 (MQ=255)
                                                                                                                                  ggcggTATATCGCTGCCCGCACGCTACCTGCGAGCAGCCTATGCCAAGGTCCGCGCCCGACGCGGGCTGTGCATCGCCGACGAAGTGCAgg                                                                            >  4:291146/1‑91 (MQ=255)
                                                                                                                                   gcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCg                 <  5:185766/149‑1 (MQ=255)
                                                                                                                                   gcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCg                 <  2:60256/149‑1 (MQ=255)
                                                                                                                                      gTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGa               >  1:20418/1‑148 (MQ=255)
                                                                                                                                       tATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGAca             <  7:344318/149‑1 (MQ=255)
                                                                                                                                          gtggCTGGCCGGATGCTACCTTCGCGGAGCCTTATCCCAGGTACGCGCCCGCGGCGGGGGTTGCTTCTCCGAAGAATTGGCGGTAGGCTATTGCCGGCTTGGGGTGTACTTTTGGGGCTTCGTCTAAAAGGGCGTGGTGCCGGAcatc           <  2:94863/145‑1 (MQ=255)
                                                                                                                                             gCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTa       <  5:293343/149‑1 (MQ=255)
                                                                                                                                              cTGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc      >  5:342965/1‑149 (MQ=255)
                                                                                                                                              cTGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc      >  1:225967/1‑149 (MQ=255)
                                                                                                                                               tGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc      >  8:86315/1‑148 (MQ=255)
                                                                                                                                                 cccGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCAt   <  2:20418/149‑1 (MQ=255)
                                                                                                                                                  ccGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATg  >  8:263995/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGCGGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATG  >  NC_002947/4694279‑4694573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑