Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,694,432 C→T 9.2% G769G (GGC→GGT)  PP_4154 → class 3 aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,694,4320CT 9.2% 214.6 / 8.3 76G769G (GGC→GGT) PP_4154class 3 aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (30/39);  new base T (3/4);  total (33/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATGGCCAAGG  >  NC_002947/4694284‑4694580
                                                                                                                                                    |                                                                                                                                                    
gCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGc                                                                                                                                                      <  5:353148/149‑1 (MQ=255)
  cGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTa                                                                                                                                                    >  5:285915/1‑149 (MQ=255)
   gCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTAc                                                                                                                                                   <  5:52926/149‑1 (MQ=255)
             ggCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAg                                                                                                                                         <  1:9495/149‑1 (MQ=255)
             ggCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAg                                                                                                                                         >  6:185766/1‑149 (MQ=255)
              gCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGc                                                                                                                                        >  6:67664/1‑149 (MQ=255)
               ccGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAGcg                                                                                                                                       <  7:108239/149‑2 (MQ=255)
                cGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAGcga                                                                                                                                      <  5:208867/149‑3 (MQ=255)
                cGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGcc                                                                                                                                       >  7:150578/1‑148 (MQ=255)
                         cgccgACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAgg                                                                                                                             >  8:62322/1‑149 (MQ=255)
                              aCTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                                        <  3:68869/149‑1 (MQ=255)
                              aCTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                                        <  6:317034/149‑1 (MQ=255)
                              aCTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                                        <  5:289845/149‑1 (MQ=255)
                                tACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGTAGCCTgcggg                                                                                                                                            >  1:282693/1‑125 (MQ=255)
                                tACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGTAGCCTgcggg                                                                                                                                            <  2:282693/127‑3 (MQ=255)
                                  ccTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgcg                                                                                                                       <  2:79057/146‑1 (MQ=255)
                                  ccTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgcg                                                                                                                       >  1:79057/1‑146 (MQ=255)
                                     gCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCcgcg                                                                                                                 >  3:70791/1‑149 (MQ=255)
                                        aGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCgcggcg                                                                                                              >  6:146970/1‑149 (MQ=255)
                                        aGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCgcggcg                                                                                                              <  1:228033/149‑1 (MQ=255)
                                          aCGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCggg                                                                                                            <  5:13879/149‑1 (MQ=255)
                                          aCGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCggg                                                                                                            <  3:269212/149‑1 (MQ=255)
                                           cGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCgggg                                                                                                           <  7:304582/149‑1 (MQ=255)
                                            gTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                          >  6:106717/1‑149 (MQ=255)
                                            gTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                          >  4:195292/1‑149 (MQ=255)
                                               gACCCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                                       <  6:357677/149‑1 (MQ=255)
                                               gACACCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                                       >  5:357677/1‑149 (MQ=255)
                                                aCGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                                       >  6:124598/1‑148 (MQ=255)
                                                    cAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCg                                                                                                  >  2:102633/1‑149 (MQ=255)
                                                       gctggctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcg                                                                                               <  7:62322/149‑1 (MQ=255)
                                                        ctggctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcga                                                                                              <  4:277761/149‑1 (MQ=255)
                                                           gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgacg                                                                                            >  2:220026/1‑148 (MQ=255)
                                                           gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgacg                                                                                            >  2:257426/1‑148 (MQ=255)
                                                           gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgac                                                                                             <  1:346283/147‑1 (MQ=255)
                                                           gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCAGCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgac                                                                                             <  6:285915/147‑1 (MQ=255)
                                                             tgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAg                                                                                         >  6:293343/1‑149 (MQ=255)
                                                             tgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAg                                                                                         >  7:166397/1‑149 (MQ=255)
                                                                 cTCGAGGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGATCCGGTGTACGGCATTGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCa                                                                                     <  4:51968/149‑1 (MQ=255)
                                                                   cGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                          <  8:228249/126‑1 (MQ=255)
                                                                   cGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                          >  7:228249/1‑126 (MQ=255)
                                                                    gACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAgg                                                                                   <  4:79726/148‑1 (MQ=255)
                                                                    gACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGt                                                                                  <  6:76239/149‑1 (MQ=255)
                                                                     aCGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAgg                                                                                   >  2:175127/1‑147 (MQ=255)
                                                                              gCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGgc                                                                        <  4:4247/149‑1 (MQ=255)
                                                                                 gTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGgccgc                                                                     <  5:106717/149‑1 (MQ=255)
                                                                                     gCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTgg                                                                 <  5:67664/149‑1 (MQ=255)
                                                                                      cTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTggg                                                                <  8:150578/149‑1 (MQ=255)
                                                                                      cTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTggg                                                                <  5:124598/149‑1 (MQ=255)
                                                                                       tGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGc                                                               >  2:66494/1‑149 (MQ=255)
                                                                                         gCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGc                                                               >  1:227576/1‑147 (MQ=255)
                                                                                         gCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGa                                                             <  8:305134/149‑1 (MQ=255)
                                                                                          cGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAg                                                            <  1:257426/149‑1 (MQ=255)
                                                                                                   tcTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTgg                                                   >  5:289878/1‑149 (MQ=255)
                                                                                                      gCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTCCTTCTGGGGc                                                >  8:152219/1‑149 (MQ=255)
                                                                                                       cGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTggg                                                  <  2:227576/146‑1 (MQ=255)
                                                                                                       cGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCt                                               >  1:60256/1‑149 (MQ=255)
                                                                                                         aaCCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTc                                             <  1:175127/149‑1 (MQ=255)
                                                                                                            cGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAg                                          <  1:66494/149‑1 (MQ=255)
                                                                                                             ggTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAgg                                         <  4:70791/149‑1 (MQ=255)
                                                                                                                     gCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCATATGCCAACGTCCGCACCCGCGGCGGGGTGTGCATCGACGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGc                                 >  1:94863/1‑149 (MQ=255)
                                                                                                                        aTGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgtg                              <  1:220026/149‑1 (MQ=255)
                                                                                                                             ggctgTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCTCCGACGAAGTGCAgg                                                                                   <  3:291146/91‑1 (MQ=255)
                                                                                                                             ggcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGcc                         <  8:125860/149‑1 (MQ=255)
                                                                                                                             ggcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGcc                         >  1:178355/1‑149 (MQ=255)
                                                                                                                             ggcggTATATCGCTGCCCGCACGCTACCTGCGAGCAGCCTATGCCAAGGTCCGCGCCCGACGCGGGCTGTGCATCGCCGACGAAGTGCAgg                                                                                   >  4:291146/1‑91 (MQ=255)
                                                                                                                              gcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCg                        <  5:185766/149‑1 (MQ=255)
                                                                                                                              gcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCg                        <  2:60256/149‑1 (MQ=255)
                                                                                                                                 gTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGa                      >  1:20418/1‑148 (MQ=255)
                                                                                                                                  tATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGAca                    <  7:344318/149‑1 (MQ=255)
                                                                                                                                     gtggCTGGCCGGATGCTACCTTCGCGGAGCCTTATCCCAGGTACGCGCCCGCGGCGGGGGTTGCTTCTCCGAAGAATTGGCGGTAGGCTATTGCCGGCTTGGGGTGTACTTTTGGGGCTTCGTCTAAAAGGGCGTGGTGCCGGAcatc                  <  2:94863/145‑1 (MQ=255)
                                                                                                                                        gCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTa              <  5:293343/149‑1 (MQ=255)
                                                                                                                                         cTGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc             >  5:342965/1‑149 (MQ=255)
                                                                                                                                         cTGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc             >  1:225967/1‑149 (MQ=255)
                                                                                                                                          tGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc             >  8:86315/1‑148 (MQ=255)
                                                                                                                                            cccGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCAt          <  2:20418/149‑1 (MQ=255)
                                                                                                                                             ccGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATg         >  8:263995/1‑149 (MQ=255)
                                                                                                                                              cGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATgg        <  3:195292/149‑1 (MQ=255)
                                                                                                                                                    cTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATGcccttgg  <  7:263995/149‑5 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATGGCCAAGG  >  NC_002947/4694284‑4694580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑