Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,694,441 C→G 6.8% R772R (CGC→CGG)  PP_4154 → class 3 aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,694,4410CG 6.8% 223.6 / 5.7 74R772R (CGC→CGG) PP_4154class 3 aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (29/39);  new base G (2/3);  total (32/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01

GGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATGGCCAAGGGCATGG  >  NC_002947/4694297‑4694586
                                                                                                                                                |                                                                                                                                                 
ggCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAg                                                                                                                                               <  1:9495/149‑1 (MQ=255)
ggCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAg                                                                                                                                               >  6:185766/1‑149 (MQ=255)
 gCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGc                                                                                                                                              >  6:67664/1‑149 (MQ=255)
  ccGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAGcg                                                                                                                                             <  7:108239/149‑2 (MQ=255)
   cGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAGcga                                                                                                                                            <  5:208867/149‑3 (MQ=255)
   cGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGcc                                                                                                                                             >  7:150578/1‑148 (MQ=255)
            cgccgACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAgg                                                                                                                                   >  8:62322/1‑149 (MQ=255)
                 aCTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                                              <  6:317034/149‑1 (MQ=255)
                 aCTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                                              <  5:289845/149‑1 (MQ=255)
                 aCTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                                              <  3:68869/149‑1 (MQ=255)
                     ccTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgcg                                                                                                                             >  1:79057/1‑146 (MQ=255)
                     ccTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgcg                                                                                                                             <  2:79057/146‑1 (MQ=255)
                        gCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCcgcg                                                                                                                       >  3:70791/1‑149 (MQ=255)
                           aGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCgcggcg                                                                                                                    >  6:146970/1‑149 (MQ=255)
                           aGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCgcggcg                                                                                                                    <  1:228033/149‑1 (MQ=255)
                             aCGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCggg                                                                                                                  <  3:269212/149‑1 (MQ=255)
                             aCGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCggg                                                                                                                  <  5:13879/149‑1 (MQ=255)
                              cGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCgggg                                                                                                                 <  7:304582/149‑1 (MQ=255)
                               gTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                                >  6:106717/1‑149 (MQ=255)
                               gTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                                >  4:195292/1‑149 (MQ=255)
                                  gACCCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                                             <  6:357677/149‑1 (MQ=255)
                                  gACACCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                                             >  5:357677/1‑149 (MQ=255)
                                   aCGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                                             >  6:124598/1‑148 (MQ=255)
                                       cAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCg                                                                                                        >  2:102633/1‑149 (MQ=255)
                                          gctggctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcg                                                                                                     <  7:62322/149‑1 (MQ=255)
                                           ctggctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcga                                                                                                    <  4:277761/149‑1 (MQ=255)
                                              gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgacg                                                                                                  >  2:257426/1‑148 (MQ=255)
                                              gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgacg                                                                                                  >  2:220026/1‑148 (MQ=255)
                                              gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgac                                                                                                   <  1:346283/147‑1 (MQ=255)
                                              gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCAGCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgac                                                                                                   <  6:285915/147‑1 (MQ=255)
                                                tgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAg                                                                                               >  7:166397/1‑149 (MQ=255)
                                                tgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAg                                                                                               >  6:293343/1‑149 (MQ=255)
                                                    cTCGAGGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGATCCGGTGTACGGCATTGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCa                                                                                           <  4:51968/149‑1 (MQ=255)
                                                      cGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                                <  8:228249/126‑1 (MQ=255)
                                                      cGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                                                >  7:228249/1‑126 (MQ=255)
                                                       gACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAgg                                                                                         <  4:79726/148‑1 (MQ=255)
                                                       gACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGt                                                                                        <  6:76239/149‑1 (MQ=255)
                                                        aCGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAgg                                                                                         >  2:175127/1‑147 (MQ=255)
                                                                 gCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGgc                                                                              <  4:4247/149‑1 (MQ=255)
                                                                    gTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGgccgc                                                                           <  5:106717/149‑1 (MQ=255)
                                                                        gCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTgg                                                                       <  5:67664/149‑1 (MQ=255)
                                                                         cTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTggg                                                                      <  5:124598/149‑1 (MQ=255)
                                                                         cTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTggg                                                                      <  8:150578/149‑1 (MQ=255)
                                                                          tGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGc                                                                     >  2:66494/1‑149 (MQ=255)
                                                                            gCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGc                                                                     >  1:227576/1‑147 (MQ=255)
                                                                            gCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGa                                                                   <  8:305134/149‑1 (MQ=255)
                                                                             cGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAg                                                                  <  1:257426/149‑1 (MQ=255)
                                                                                      tcTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTgg                                                         >  5:289878/1‑149 (MQ=255)
                                                                                         gCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTCCTTCTGGGGc                                                      >  8:152219/1‑149 (MQ=255)
                                                                                          cGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTggg                                                        <  2:227576/146‑1 (MQ=255)
                                                                                          cGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCt                                                     >  1:60256/1‑149 (MQ=255)
                                                                                            aaCCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTc                                                   <  1:175127/149‑1 (MQ=255)
                                                                                               cGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAg                                                <  1:66494/149‑1 (MQ=255)
                                                                                                ggTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAgg                                               <  4:70791/149‑1 (MQ=255)
                                                                                                        gCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCATATGCCAACGTCCGCACCCGCGGCGGGGTGTGCATCGACGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGc                                       >  1:94863/1‑149 (MQ=255)
                                                                                                           aTGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgtg                                    <  1:220026/149‑1 (MQ=255)
                                                                                                                ggctgTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCTCCGACGAAGTGCAgg                                                                                         <  3:291146/91‑1 (MQ=255)
                                                                                                                ggcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGcc                               <  8:125860/149‑1 (MQ=255)
                                                                                                                ggcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGcc                               >  1:178355/1‑149 (MQ=255)
                                                                                                                ggcggTATATCGCTGCCCGCACGCTACCTGCGAGCAGCCTATGCCAAGGTCCGCGCCCGACGCGGGCTGTGCATCGCCGACGAAGTGCAgg                                                                                         >  4:291146/1‑91 (MQ=255)
                                                                                                                 gcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCg                              <  5:185766/149‑1 (MQ=255)
                                                                                                                 gcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCg                              <  2:60256/149‑1 (MQ=255)
                                                                                                                    gTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGa                            >  1:20418/1‑148 (MQ=255)
                                                                                                                     tATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGAca                          <  7:344318/149‑1 (MQ=255)
                                                                                                                        gtggCTGGCCGGATGCTACCTTCGCGGAGCCTTATCCCAGGTACGCGCCCGCGGCGGGGGTTGCTTCTCCGAAGAATTGGCGGTAGGCTATTGCCGGCTTGGGGTGTACTTTTGGGGCTTCGTCTAAAAGGGCGTGGTGCCGGAcatc                        <  2:94863/145‑1 (MQ=255)
                                                                                                                           gCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTa                    <  5:293343/149‑1 (MQ=255)
                                                                                                                            cTGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc                   >  5:342965/1‑149 (MQ=255)
                                                                                                                            cTGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc                   >  1:225967/1‑149 (MQ=255)
                                                                                                                             tGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc                   >  8:86315/1‑148 (MQ=255)
                                                                                                                               cccGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCAt                <  2:20418/149‑1 (MQ=255)
                                                                                                                                ccGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATg               >  8:263995/1‑149 (MQ=255)
                                                                                                                                 cGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATgg              <  3:195292/149‑1 (MQ=255)
                                                                                                                                       cTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATGcccttgg        <  7:263995/149‑5 (MQ=255)
                                                                                                                                           cTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATGGCCAAGGGCAt    >  3:219421/1‑149 (MQ=255)
                                                                                                                                              cgcgcAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATGGCCAAGGGCATgg  <  6:289878/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTACCATGGCCAAGGGCATGG  >  NC_002947/4694297‑4694586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑